A kind of Pediococcus pentosacea and its application, screening and testing method

A technology of Pediococcus pentosaceus and microbial strains, applied in the field of microorganisms, can solve the problems of single variety of lactic acid bacteria and cannot meet market demand, etc., and achieve the effects of improving quality, reducing the production of ammonia nitrogen content, and reducing pH value.

Active Publication Date: 2019-01-18
四川农科源检测技术有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microorganisms and convert limited sugar, reduce the pH value of silage, and improve the quality of silage. Different silage materials have different fermentation characteristics, and the current lactic acid bacteria suitable for silage are single, which cannot meet market demand

Method used

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  • A kind of Pediococcus pentosacea and its application, screening and testing method
  • A kind of Pediococcus pentosacea and its application, screening and testing method
  • A kind of Pediococcus pentosacea and its application, screening and testing method

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Experimental program
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Effect test

Embodiment 2

[0048] Physiological and biochemical assays: Analyze the physiological and biochemical characteristics of Pediococcus pentosaceae in Example 1, the results are shown in Table 2.

[0049] Different temperature test: Pediococcus pentosaceae in Example 1 was inoculated into the MRS liquid medium with an inoculum size of 3%, cultured under the temperature conditions in Table 2, and its growth status was observed. The results are shown in Table 2.

[0050] Salt tolerance test: with the Pediococcus pentosaceae in embodiment 1 in the MRS liquid culture medium that contains NaCl concentration 3.0% (w / v) and 6.5% (w / v), after cultivating 2 days at 30 ℃, measure its tolerance Salinity, the results are shown in Table 2.

[0051] Acid-resistant test: After the Pediococcus pentosaceae in Example 1 was cultured at 30° C. for 7 days in the MRS liquid medium with the pH value in Table 2, its acid resistance was measured, and the results are shown in Table 2.

[0052] Sugar fermentation test:...

Embodiment 3

[0065] Using Pediococcus pentosaceae of the present invention as pasture silage additive

[0066] Forage grass with a height of 1.5-2m was collected from the Chongzhou base of Sichuan Agricultural University, cut into a length of about 20mm, and fresh samples were obtained. For each treatment, 300g of fresh samples were taken and put into plastic bags (25×35cm; Aodeju, China). Pediococcus pentosaceae of the present invention in example 1 1.0 * 10 8 Add 1ml of CFU / g fresh material to each bag of silage, then vacuum seal it, place it at room temperature to ferment, each treatment group has 3 repetitions, and open the bag after 60 days of silage to obtain silage samples.

[0067] 1. Sensory evaluation:

[0068] 1) Silage samples According to the sensory silage scoring standard and grade evaluation compiled by the German Farming Association (DLG) in 1899, sensory scores were performed on the smell, structure and color of the silage.

[0069] Table 4 Sensory Evaluation Criteria f...

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Abstract

The invention discloses a Pediococcus pentosaceus and its application and screening method. The Pediococcus pentosaceus is preserved in the General Microbiology Center of China Committee for the Preservation of Microorganisms, and the preservation number is CGMCC No.15074; The Pediococcus pentosaceae has a fast growth rate and high acid production efficiency; the Pediococcus pentosaceae is used as a forage silage additive to improve the quality of the forage silage, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Pediococcus pentosaceae and its application and screening assay method. Background technique [0002] Silage is a storage method that converts the fermentation substrate in raw materials into acidic substances such as lactic acid through the proliferation of lactic acid bacteria, and maintains an acidic anaerobic environment to facilitate the long-term preservation of silage crops. Silage is yellow-green in color, sour in smell, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding, and has become an important source of high-quality roughage. [0003] Lactic acid bacteria is a silage microbial additive that is being promoted in recent years. Its main function is to adjust the composition of microorganisms in silage materials, quickly become the dominant flora, compete to inhibit the growth of harmful microo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/04C12Q1/689A23K10/30A23K10/12C12R1/01
CPCA23K10/12A23K10/30C12N1/02C12Q1/689C12N1/205C12R2001/01
Inventor 张新全关皓闫艳红李小铃帅杨李小梅黄琳凯聂刚汪霞黄婷
Owner 四川农科源检测技术有限公司
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