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Young shoot grafting method for virus removal of apples

A technology for apples and young shoots, which is applied in the field of apple virus-free young shoot grafting, can solve the problems of difficult operation, low survival rate of shoot tip grafting in tissue culture, and inability to remove viruses, etc., to break through seasonal restrictions, improve grafting survival rate, The effect of reducing the probability of carriage and transmission

Inactive Publication Date: 2018-05-29
北京市林业果树科学研究院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of grafting propagation method with strong operability, high survival rate and not easy to carry virus for the existing apple grafting technology which is easy to carry plant virus and the survival rate of tissue culture stem tip grafting is low. Virus-free treatment of the female parent plant, detection of virus-free status of new shoots, removal of the tips of the new shoots that are not infected by the virus, and then grafting and reproduction, which solves the problem that conventional grafting cannot remove the virus and the survival rate of shoot tip grafting is low It can significantly improve the grafting survival rate of virus-free materials, so as to quickly obtain a large number of virus-free plants, and then provide provenance and reproductive technology guarantee for virus-free breeding and virus-free cultivation of apple seedlings

Method used

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  • Young shoot grafting method for virus removal of apples

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Experimental program
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Effect test

Embodiment 1

[0035] Practical Application of Example 1 Apple Virus-free Tip Grafting Method

[0036] On July 16, 2017, the virus-free apple plants were grafted.

[0037] The apple scion varieties used were Gala, Kudo Fuji, 2001 Fuji, Yanfu 3, Yanfu 0, Yanfu 8, Shinano Gold, New Jona Gold, Shizuka, SH6, M9-T337, and the non-toxic rootstock used was G935. The grafting method used is skin grafting, and the tip grafting process can be found in figure 1 ,details as follows:

[0038] (1) Branch scissors, grafting knives and other utensils need to be disinfected first, and the ground diameter of spare non-toxic rootstock G935 seedlings is ≥1.5cm;

[0039] (2) Gala apple female parent potted plants are treated at 37°C for 30 days, take leaves at 4-5cm from the top of the new shoot to detect the virus, mark the detection site, and detect the non-toxic new shoot at a distance of 3-4cm from the tip. Cut off the original poisonous plants with non-toxic pruning shears;

[0040] (3) Grafting by inse...

Embodiment 2

[0044] Embodiment 2 tip grafting method of the present invention is compared with common tip grafting method

[0045] On August 10, 2017, the virus-free apples were grafted.

[0046] The apple scion variety used is Gala, the rootstock used is virus-free G935, and the grafting method used is grafting by cuttings. The implementation process of the patented method is the same as the previous example, and the traditional method is implemented with reference to "Introduction to Fruit Tree Cultivation" (Higher Education Press, 1999) 50 strains were grafted each, and the survival rate and the virus-free rate were counted after 50 days.

[0047] Through the 3.0-4.0cm virus-free apple tip grafting technology provided by this patent, a total of 50 plants were grafted, 42 survived, and the grafting survival rate reached 84%; 35 virus-free seedlings were obtained, with a virus-free rate of 70%. A total of 50 plants were grafted by the common tip grafting method, and 12 plants survived, w...

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Abstract

The invention provides a young shoot grafting method for virus removal of apples. The method comprises the following steps: disinfecting grafting tools in advance; detecting virus carrying conditionsof leaves located at 4-5cm the top of a new shoot which is grown to 15-20cm and treated by virus removal, retaining 1-2 young leaves on the top end of the new shoot of which target virus is removed, and cutting a shoot tip with the length of 3-4cm to be used as a scion; selecting a potted virus-free annual growth rootstock seedling with the main stem diameter of 1.5cm or more to be used as a virus-free rootstock, performing rootstock cutting at the height of 25-35cm far away from the ground; and after grafting is performed, performing moisturizing by performing bagging on the shoot tip and a connection opening part, performing moisturizing culture in a phytotron for 10-15 days, and performing seedling exercising to obtain a virus-free seedling of apples. According to the method provided bythe invention, shoot tips obtained by special ways such as an insufficient maturity degree, seasonal limitation, tissue culture, and anti-seasonal seed introduction can perform apple seedling grafting, preservation and reproduction at any time, the problem of a low survival rate of young shoot grafting in a conventional grafting method is solved, and a novel method is opened up for reproduction of virus-free seedlings.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for grafting virus-free young shoots of apples. Background technique [0002] Apple virus disease is one of the main factors seriously affecting fruit yield and quality. After the apple tree is infected by the virus, it will carry the virus for life. The virus will multiply in the fruit tree, interfere with and destroy the normal physiological functions of the tree, resulting in reduced growth, reduced yield, poor quality, and intolerance to storage, causing serious harm to apple production. At present, there are more than 40 kinds of apple viruses that have been discovered and identified, among which the ones that cause major harm worldwide mainly include apple chloroticleaf spot virus (ACLSV), apple stem grooving virus (ASGV), and apple stem grooving virus (ASGV). ), apple stem pitting virus (ASPV), Prunus necrotic ringspotilarvirus (PNRSV), apple scar ski...

Claims

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Application Information

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IPC IPC(8): A01G2/30A01G2/38
Inventor 张军科李兴亮周佳周贝贝张钰岷张强李民吉杨雨璋魏钦平
Owner 北京市林业果树科学研究院
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