Stably preserved RNA (ribonucleic acid) quality control serum of lung cancer fusion gene detection kit

A detection kit and gene fusion technology, which is applied in the field of RNA quality control products, can solve the problems of easy degradation, difficulty and instability, and achieve the effects of ensuring accuracy, low use cost, monitoring accuracy and stability

Active Publication Date: 2018-05-29
GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A variety of lung cancer fusion gene detection kits have been developed based on the above methods, but there are currently no economical, effective, stable and universal quality control products for lung cancer fusion gene detection kits on the market, and the existing RNA quality c

Method used

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  • Stably preserved RNA (ribonucleic acid) quality control serum of lung cancer fusion gene detection kit
  • Stably preserved RNA (ribonucleic acid) quality control serum of lung cancer fusion gene detection kit
  • Stably preserved RNA (ribonucleic acid) quality control serum of lung cancer fusion gene detection kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] An embodiment of the RNA quality control product of the lung cancer fusion gene detection kit of the present invention includes a positive reference product, a negative reference product and a detection limit reference product.

[0043] The positive reference product comprises an ALK fusion gene reference product, a ROS1 fusion gene reference product and a RET fusion gene reference product. The ALK fusion gene reference product comprises ALK-EML4, ALK-KIF5B, ALK-KLC1, ALK-TFG, ALK-TPR, ALK-HIP1, ALK-STRN, ALK-DCTN1, ALK-SQSTM1, ALK-BIRC6 and ALK- CLTC fusion gene reference product; the ROS1 fusion gene reference product includes ROS1-CD74, ROS1-SLC34A2, ROS1-GOPC, ROS1-SDC4, ROS1-TPM3, ROS1-EZR, ROS1-LRIG3, ROS1-KDELR2, ROS1-LIMA1 , ROS1-MSN, and ROS1-TMEM106B fusion gene reference products; the RET fusion gene reference products include KIF5B-RET and RET-CCDC6 fusion gene reference products. The fusion gene reference products respectively contain the corresponding fus...

Embodiment 2

[0047] An embodiment of the RNA quality control product of the lung cancer fusion gene detection kit of the present invention includes a positive reference product, a negative reference product and a detection limit reference product.

[0048] The positive reference product comprises an ALK fusion gene reference product, a ROS1 fusion gene reference product and a RET fusion gene reference product. The ALK fusion gene reference products include ALK-EML4, ALK-KIF5B, ALK-KLC1 and ALK-BIRC6 fusion gene reference products; the ROS1 fusion gene reference products include ROS1-MSN, ROS1-EZR and ROS1-TMEM106B fusion gene reference products product; the RET fusion gene reference product comprises a KIF5B-RET fusion gene reference product. The fusion gene reference products respectively contain the corresponding fusion gene RNA, aurin tricarboxylate ammonium salt 500 μM, glycine 180 mM and bestatin hydrochloride 60 nM.

[0049] The negative reference product comprises an AML1-ETO fusio...

Embodiment 3

[0052] An embodiment of the RNA quality control product of the lung cancer fusion gene detection kit of the present invention includes a positive reference product, a negative reference product and a detection limit reference product.

[0053] The positive reference product comprises an ALK fusion gene reference product and a ROS1 fusion gene reference product. The ALK fusion gene reference product comprises ALK-EML4, ALK-KIF5B, ALK-KLC1, ALK-TFG, ALK-TPR, ALK-HIP1, ALK-STRN, ALK-DCTN1, ALK-SQSTM1, ALK-BIRC6 and ALK- CLTC fusion gene reference product; the ROS1 fusion gene reference product includes ROS1-CD74, ROS1-SLC34A2, ROS1-GOPC, ROS1-SDC4, ROS1-TPM3, ROS1-EZR, ROS1-LRIG3, ROS1-KDELR2, ROS1-LIMA1 , ROS1-MSN, and ROS1-TMEM106B fusion gene reference product; said fusion gene reference product contains corresponding fusion gene RNA, aurin tricarboxylate ammonium salt 400 μ M, glycine 110 mM and bestatin hydrochloride 35 nM respectively.

[0054] The negative reference produ...

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Abstract

The invention discloses a stably preserved RNA (ribonucleic acid) quality control serum of a lung cancer fusion gene detection kit. The quality control serum contains the following ingredients: a positive reference, a negative reference and a detection limit reference. The positive reference, the negative reference and the detection limit reference respectively contain a corresponding fusion geneRNA, aurin ammonium triformate salt, a preservative and bestatin hydrochloride; the preservative is selected from glycine, epsilon-polylysine or cysteine. According to the RNA quality control serum provided by the invention, free RNAs can be effectively prevented from being degraded, a stable environment is provided for the RNAs, and the preservation time of the RNAs is prolonged; meanwhile, the RNA quality control serum has the advantages of being low in preparation cost, stable in performance, easy in preservation and the like; the factors such as personnel operation, instrument states, theeffectiveness of the kit and the like can be effectively monitored, so that the accuracy and reliability of the detection kit can be greatly improved.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to an RNA quality control product of a lung cancer fusion gene detection kit that can be stored stably. Background technique [0002] Lung cancer is a malignant tumor with the highest morbidity and mortality worldwide, which seriously endangers human health. According to the global cancer epidemiological data in 2012, there are approximately 14.1 million new cancer cases and 8.2 million deaths each year in the world. Among them, 1.825 million cases of lung cancer and 1.59 million deaths rank first. Lung cancer is divided into non-small cell lung cancer (NSCLC) and small cell lung cancer, and NSCLC accounts for about 80% of all lung cancers. Chromosomal rearrangement and gene rearrangement are common genetic changes in malignant tumors. In 2007, Soda et al. first discovered the fusion of echinoderm microtubule-associated protein-like 4 (EML4) gene and anaplastic lymphoma ki...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/166
Inventor 陈嘉昌陈肖燕柳俊蒋圆玲张瑶
Owner GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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