Stably preserved RNA (ribonucleic acid) quality control serum of lung cancer fusion gene detection kit

Abstract

The invention discloses a stably preserved RNA (ribonucleic acid) quality control serum of a lung cancer fusion gene detection kit. The quality control serum contains the following ingredients: a positive reference, a negative reference and a detection limit reference. The positive reference, the negative reference and the detection limit reference respectively contain a corresponding fusion geneRNA, aurin ammonium triformate salt, a preservative and bestatin hydrochloride; the preservative is selected from glycine, epsilon-polylysine or cysteine. According to the RNA quality control serum provided by the invention, free RNAs can be effectively prevented from being degraded, a stable environment is provided for the RNAs, and the preservation time of the RNAs is prolonged; meanwhile, the RNA quality control serum has the advantages of being low in preparation cost, stable in performance, easy in preservation and the like; the factors such as personnel operation, instrument states, theeffectiveness of the kit and the like can be effectively monitored, so that the accuracy and reliability of the detection kit can be greatly improved.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to an RNA quality control product of a lung cancer fusion gene detection kit that can be stored stably. Background technique [0002] Lung cancer is a malignant tumor with the highest morbidity and mortality worldwide, which seriously endangers human health. According to the global cancer epidemiological data in 2012, there are approximately 14.1 million new cancer cases and 8.2 million deaths each year in the world. Among them, 1.825 million cases of lung cancer and 1.59 million deaths rank first. Lung cancer is divided into non-small cell lung cancer (NSCLC) and small cell lung cancer, and NSCLC accounts for about 80% of all lung cancers. Chromosomal rearrangement and gene rearrangement are common genetic changes in malignant tumors. In 2007, Soda et al. first discovered the fusion of echinoderm microtubule-associated protein-like 4 (EML4) gene and anaplastic lymphoma ki...

AI Technical Summary

Problems solved by technology

A variety of lung cancer fusion gene detection kits have been developed based on the above methods, but there are currently no economical, effective, stable and universal quality control products for lung cancer fusion gene detection kits on the market, and the existing RNA quality control categories Most of the products have shortcomings such as instability, easy degradation, and difficult storage, and quality control products are important methods and quality indicators to measure and evaluate the performance of a kit such as detection accuracy and stability.

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