Autoimmunity detection kit with enzyme-linked immuno spot blotting and operation process thereof

Abstract

The invention discloses an autoimmunity detection kit with enzyme-linked immuno spot blotting and an operation process thereof. The autoimmunity detection kit with the enzyme-linked immuno spot blotting comprises a kit body, target molecules and a reagent. The kit is a technology based on combination of enzyme-linked immuno spot blotting and enrichment and is used for detecting pathoglycemia caused by deficiency of the autoimmunity function. The operation process of the autoimmunity detection kit with the enzyme-linked immuno spot blotting comprises the following steps of: preparing the targetmolecules, preparing a covering device of the target molecules, preparing cell lysis solution, preparing anti-human immune cell antibodies, enriching the immune cells, preparing amplification culturemedium, detecting the target molecules and analyzing clinical-sample detection data. The autoimmunity detection kit and the operation process disclosed by the invention have the beneficial effects that by combination of the immune cell enrichment technology and the enzyme-linked immuno spot blotting technology with high sensitivity and high specificity and accurate analysis for the correspondingtarget molecules, the detection specificity is improved and the false positive probability is reduced.

Description

technical field [0001] The invention relates to the technical field of specific auxiliary diagnosis of type 1 diabetes, in particular to an enzyme-linked immunospot blot autoimmune detection kit and its operation process. Background technique [0002] Diabetes is one of the most serious diseases threatening human health today. According to the report of the World Health Organization, the global prevalence of diabetes among adults over 18 years old increased from 4.7% in 1980 to 8.5% in 2014. In recent years, with the improvement of living conditions, the prevalence of diabetes in China has also increased year by year, and the total number of patients has reached nearly 100 million. Especially in some relatively developed areas, the number of cases is increasing year by year, and there is also a trend of younger people. [0003] Diabetes mellitus is a metabolic disease characterized by hyperglycemia (fasting blood glucose above 7.0 mmol / L). Hyperglycemia is caused by defect...

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Problems solved by technology

[0006] Purpose of the invention: Aiming at the defects and insufficiencies of the prior art, to solve the problems of poor detection specificity, high probability of false positives, and poor accuracy, the applicant has designed an autoimmune detection method of ELISA dot blot after many practice improvements. Kit and its operating procedure

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