Screening method of non-invasive gastric cancer saliva biomarker

A technology of biomarkers and screening methods, applied in the field of screening of non-invasive gastric cancer salivary biomarkers, can solve different problems

Inactive Publication Date: 2018-06-12
SHENZHEN ELDERLY MEDICAL RES INST +1
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  • Claims
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Problems solved by technology

At present, Japan has used PG as a tumor marker for early diagnosis and screening of gastric cancer, but there is no large-scale evidence to support it in my country, and the level of PG in serum is affected by various factors (regional differences, dietary structure, rac

Method used

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  • Screening method of non-invasive gastric cancer saliva biomarker
  • Screening method of non-invasive gastric cancer saliva biomarker

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Embodiment Construction

[0166] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0167] see figure 1 , a method for screening non-invasive gastric cancer salivary biomarkers, the specific steps are as follows:

[0168] (1) Preparation of protein samples: Add 600 μL of 1X lysate to the frozen saliva protein samples, sonicate for 2 seconds and stop for 3 seconds, and sonicate for 2 minutes at Amp 20% until the samples are completely dissolved and become clear; After continuing to lyse on ice for 30 minutes, centrifuge at 20,000g for 40 minutes with a centrifuge pre-cooled to 4°C in advance; discard the precipitate and take the supernatant, add it to a 3kDa ultrafiltration tube, and centrifuge at 14,000g with a 4°C centrifuge Concentrate the protein for 30 minutes; after completion, add 200 μL of 1X lysate to the ultrafiltration tube, and perform ultrafiltration at 14,000 g for 20 minutes at 4°C, and repeat 3 times; 20°C refrige...

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Abstract

The invention discloses a screening method of a non-invasive gastric cancer saliva biomarker. The screening method specifically comprises the following steps: protein sample preparation, protein quantification, proteolysis and freeze-drying, labeling a sample by iTRAQ, C18 desalting, liquid-phase separation and mass spectrum identification, and data analysis; the GO function enrichment including biology progress, the molecule function and the cell component is performed on the screened differential protein by using the Panther Classification System in the data analysis; and then the protein mutual effect analysis is performed on the differential protein by using the STRING analysis software; and then the KEGG signal path analysis is performed on the differential protein. Through the screening method disclosed by the invention, the specificity protein marker in the gastric cancer can be discovered through a fast and reliable proteomics technical program, thereby establishing a non-invasive, simple and convenient, fast and practical detection evaluation measure for the early diagnosis, the postoperative recurrence, metastasis, and prognosis observation of the gastric cancer.

Description

technical field [0001] The invention relates to a screening method for biomarkers, in particular to a screening method for non-invasive gastric cancer salivary biomarkers. Background technique [0002] Gastric cancer is one of the most common malignant gastrointestinal tumors worldwide. The onset of gastric cancer is hidden, and the early symptoms and signs are not obvious, so it needs to rely on auxiliary examinations for diagnosis. At present, routine gastroscopy and biopsy of pathological tissue obtained under the microscope are recognized as the gold standard for the diagnosis of gastric cancer, but it is still difficult to carry out large-scale census in my country. Extensive screening programs of asymptomatic individuals by gastric fluoroscopy have increased the number of early gastric cancer cases detected, increasing the overall 5-year survival rate to over 50%. It has good sensitivity and specificity, however, cost-effectiveness studies indicate that gastric fluor...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57446G01N33/57484G01N33/6893
Inventor 吴正治黄飞娟范大华姚永超曹美群孙珂焕丁峰
Owner SHENZHEN ELDERLY MEDICAL RES INST
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