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Preparation method for composite freeze-dried powder with multiple stem cells and solvent

A technology of freeze-dried powder and stem cells, applied in the biological field, can solve the problems of high operator, psychological burden and safety problems for beauty lovers

Active Publication Date: 2018-07-13
伯仕利生物科技发展(盐城)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional stem cell cosmetology needs to inject stem cells into the dermis of the skin, which has high requirements for the operator, and this operation is likely to cause psychological burden and safety concerns for beauty lovers

Method used

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  • Preparation method for composite freeze-dried powder with multiple stem cells and solvent
  • Preparation method for composite freeze-dried powder with multiple stem cells and solvent

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Experimental program
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Effect test

Embodiment

[0036] A preparation method for composite freeze-dried powder of multiple stem cells and a solvent, comprising the following steps:

[0037] (1) Isolation and culture of umbilical cord and placental mesenchymal stem cells

[0038] Under sterile conditions, collect healthy umbilical cord and placenta; cut the placenta to 1.5-2.5mm in GMP laboratory biosafety cabinet 3 Tissue pieces of different sizes were placed in DMEM-LG cell culture medium contained in T175 culture flasks at 37°C and 5% CO 2 Cultivated in an incubator;

[0039] Change the medium every 2-3 days until a large number of stem cells crawl out, discard the tissue pieces; when the cells are full, record it as the first generation, digest and passage with 0.28% trypsin; after the second generation, use no antibiotics DMEM-LG medium for culture; sequential subculture;

[0040] (2) Isolation and culture of adipose-derived mesenchymal stem cells

[0041] Under sterile conditions, obtain 100 mg of adipose tissue fro...

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Abstract

The invention discloses a preparation method for composite freeze-dried powder with multiple stem cells and a solvent. The preparation method comprises the following steps of: utilizing a health tissue block method to culture mesenchymal stem cells of umbilical cords and placentas, then using a digestion method to culture adipose-derived stem cells, blending cell factors secreted in the culture process, finding an optimal growth concentration by a fibroblast proliferation curve test, carrying out low-temperature freezing and drying on blended culture supernatant, and preparing a suitable solvent; as a raw material of a beauty product, the solvent can be added into other beauty products in the later stage to prepare stem-cell beauty products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method for multiple stem cell composite freeze-dried powder and a solvent. Background technique [0002] With the gradual improvement of people's material living standards, it is no longer a problem for people to have enough food and clothing. The affluence of life makes people start to pursue a higher level of life. Everyone has a desire for beauty, and everyone hopes to be young and beautiful forever. Many people turn their attention to various methods that can improve their appearance. As the largest tissue and organ of the human body, the skin covers the appearance of the human body and is the main tissue and organ for the study of aesthetics and cosmetology. All of a sudden, various cosmetics and skin care products have sprung up like mushrooms after rain. However, these cosmetics on the market only achieve the purpose of beauty by covering up defects or acting on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/02A61K8/99A61Q19/00C12N5/0775
CPCA61K8/022A61K8/99A61Q19/00C12N5/0665C12N5/0667C12N2509/00
Inventor 吴金芸张炬
Owner 伯仕利生物科技发展(盐城)有限公司
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