Application of Paeoniflorin Metabolin I in the Preparation of Drugs for Colitis

A technology for paeoniflorin metabolite and colitis, which is applied in directions such as drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as unreported activity of paeoniflorin metabolite I and the like, and achieves cost-effectiveness and convenience in use. Effect

Active Publication Date: 2021-03-19
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of paeoniflorin metabolin I has not been reported

Method used

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  • Application of Paeoniflorin Metabolin I in the Preparation of Drugs for Colitis
  • Application of Paeoniflorin Metabolin I in the Preparation of Drugs for Colitis
  • Application of Paeoniflorin Metabolin I in the Preparation of Drugs for Colitis

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Experimental program
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Embodiment 1

[0044] 1.MTT experiment

[0045] The thymus tissue of C57BL / 6 adult mice was taken, and the mature lymphocytes of the mice were obtained by grinding and extracting for culture. The normal lymphocytes in logarithmic growth phase were inoculated into 96-well plates for MTT test, and 1 × 10 cells were inoculated in each well. 5 cell. Paeoniflorin was used as the control group, and the added concentration was 0.25mg / ml, 0.5mg / ml g, 1mg / ml, 2mg / ml, 4mg / ml; the experimental group was paeoniflorin metabolite I, and the added concentration was 1.25μg / ml, 2.5 μg / ml, 5μg / ml, 10μg / ml and 20μg / ml, three wells in parallel for each group. After 72 hours of treatment, MTT was added and measured with a microplate reader. The results showed that the IC50 value of paeoniflorin on normal cells was 1 mg / ml, and the IC50 value of paeoniflorin metabolite I on normal lymphocytes was 5 μg / ml.

[0046] 2. Animal modeling experiments

[0047] 2.1 Establishment of colitis model in C57BL / 6 mice

[00...

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Abstract

The invention researches the anti-inflammatory activity of DSS (Dextran Sulfate Sodium) inducted colitis model for paeoniflorin and paeoniflorin metabolism pigment I. An experiment result indicates that the paeoniflorin metabolism pigment I has a good treatment effect on ulcerative colitis. Compared with a control group and a paeoniflorin group, a C57BL / 6 mouse which orally takes the paeoniflorinmetabolism pigment I, the paeoniflorin metabolism pigment I is characterized in that a colon tissue damage degree is lightened, the mouse colon tissue damage degree of the low and high dosage medication administration team of the paeoniflorin metabolism pigment I is lightened, a tissue structure is clear in level, the infiltration of inherent layer inflammatory cells is reduced, the amount of cup-shaped cells is relatively increased, muscularis mucosa does not contain tissue edema and hyperaemia, and tissue forms are obviously recovered. The paeoniflorin metabolism pigment I is proved to havea good treatment effect on the ulcerative colitis.

Description

technical field [0001] The invention relates to the field of drugs for treating colitis, in particular to the application of paeoniflorin metabolite I in the preparation of drugs for treating colitis. Background technique [0002] Colitis refers to inflammatory lesions of the colon caused by various reasons. It can be caused by organisms such as bacteria, fungi, viruses, parasites, and protozoa, and can also be caused by allergic reactions and physical and chemical factors. According to different causes, it can be divided into specific inflammatory lesions and non-specific inflammatory lesions. The former refers to infectious colitis, Ischemic colitis and pseudomembranous colitis, the latter including ulcerative colitis and colonic Crohn's disease. [0003] Ulcerative colitis is one of the more common clinical diseases in gastroenterology. In recent years, with the development of national economy and the improvement of quality of life, the incidence of ulcerative colitis ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/357A61P1/00A61P1/04A61P7/10
CPCA61K31/357A61P1/00A61P1/04A61P7/10
Inventor 段文娟冯金红李月杨国红王晓许亦真耿岩玲王岱杰
Owner SHANDONG ANALYSIS & TEST CENT
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