Application of Paeoniflorin Metabolin I in the Preparation of Drugs for Colitis
A technology for paeoniflorin metabolite and colitis, which is applied in directions such as drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as unreported activity of paeoniflorin metabolite I and the like, and achieves cost-effectiveness and convenience in use. Effect
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[0044] 1.MTT experiment
[0045] The thymus tissue of C57BL / 6 adult mice was taken, and the mature lymphocytes of the mice were obtained by grinding and extracting for culture. The normal lymphocytes in logarithmic growth phase were inoculated into 96-well plates for MTT test, and 1 × 10 cells were inoculated in each well. 5 cell. Paeoniflorin was used as the control group, and the added concentration was 0.25mg / ml, 0.5mg / ml g, 1mg / ml, 2mg / ml, 4mg / ml; the experimental group was paeoniflorin metabolite I, and the added concentration was 1.25μg / ml, 2.5 μg / ml, 5μg / ml, 10μg / ml and 20μg / ml, three wells in parallel for each group. After 72 hours of treatment, MTT was added and measured with a microplate reader. The results showed that the IC50 value of paeoniflorin on normal cells was 1 mg / ml, and the IC50 value of paeoniflorin metabolite I on normal lymphocytes was 5 μg / ml.
[0046] 2. Animal modeling experiments
[0047] 2.1 Establishment of colitis model in C57BL / 6 mice
[00...
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