Method and system for analyzing interaction relationship between populus tomentosa LncRNA Pto-CRTG and target gene Pto-CAD5 thereof by utilizing epistasis

A technology of lncrnapto-crtg and pto-cad5, applied in the field of genetics research

Active Publication Date: 2018-09-11
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the current research on the function of lncRNA is still in the preliminary stag...

Method used

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  • Method and system for analyzing interaction relationship between populus tomentosa LncRNA Pto-CRTG and target gene Pto-CAD5 thereof by utilizing epistasis
  • Method and system for analyzing interaction relationship between populus tomentosa LncRNA Pto-CRTG and target gene Pto-CAD5 thereof by utilizing epistasis
  • Method and system for analyzing interaction relationship between populus tomentosa LncRNA Pto-CRTG and target gene Pto-CAD5 thereof by utilizing epistasis

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Embodiment

[0080] Raw material acquisition: the individuals with the highest growth and the lowest growth of Populus tomentosa came from a hybrid population of Populus tomentosa (Populus tomentosa “TB14” × Populus tomentosa “ Pt-3"); the natural population of Populus tomentosa used for association mapping analysis comes from the national P. tomentosa germplasm resource bank, which represents the natural distribution of P. tomentosa across the country;

[0081] Reagents used in the polymerase chain reaction (PCR) system were purchased from Promega;

[0082] Combine Tophat and Cufflink package system for lncRNA prediction and analysis;

[0083] Epistasis association analysis was performed using EPISNP software.

[0084] The specific operation steps are as follows:

[0085] Step S1, in the Populus tomentosa hybrid population composed of 736 individuals of Populus tomentosa, according to the comprehensive evaluation results of tree height, diameter at breast height and volume, respectively...

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Abstract

The invention provides a method and a system for analyzing an interaction relationship between populus tomentosa LncRNA Pto-CRTG and a target gene Pto-CAD5 thereof by utilizing epistasis. According tothe method and the system of embodiments of the invention, the interaction relationship between the populus tomentosa LncRNA Pto-CRTG and the target gene Pto-CAD5 thereof can be analyzed by utilizingthe epistasis. Functional SNP sites found in the LncRNA Pto-CRTG and the target gene Pto-CAD5 thereof provide important reference value for the application of the LncRNA Pto-CRTG and the target genePto-CAD5 thereof in forest tree molecular marker breeding.

Description

technical field [0001] The present invention relates to the field of genetics research, specifically, the present invention relates to a method and a system for analyzing the interaction relationship between Populus tomentosa LncRNA Pto-CRTG and its target gene Pto-CAD5 by using epistasis. Background technique [0002] Long non-coding RNA (Long non-coding RNA, LncRNA) is a kind of long non-coding RNA molecules with a length of more than 200nt, which is a by-product of RNA polymerase II transcription. According to its position on the genome, it can be divided into four main types: sense lncRNA (sense lncRNA), antisense lncRNA (antisense lncRNA), intragenic lncRNAs (intronic lncRNA), and intergenic lncRNAs (intergenic lncRNA). According to statistics, mammalian protein-coding genes account for 1% of total RNA, while long-chain non-coding RNA accounts for 4% to 9%. These long-chain non-coding RNAs have become research hotspots after microRNA. A large number of studies have sho...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12M1/34
CPCC12Q1/6895C12Q2600/156C12Q2600/158C12Q2600/178
Inventor 张德强周大凌杜庆章
Owner BEIJING FORESTRY UNIVERSITY
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