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Low amylose content gene wx-C39 functional marker and application thereof

A low-amylose, wx-c39 technology, applied in the field of agricultural bioengineering, can solve the problems of poor eating quality, high gelatinization temperature, low viscosity, etc., and achieve high accuracy, low cost and high detection efficiency. Effect

Active Publication Date: 2018-09-14
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Abstract
  • Description
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Problems solved by technology

wxya a Mainly distributed in indica rice varieties, controlling the synthesis of high amylose content, the amylose content of rice is generally 20-25%, the gelatinization temperature of rice is high, the viscosity is low, and the eating quality is poor

Method used

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  • Low amylose content gene wx-C39 functional marker and application thereof
  • Low amylose content gene wx-C39 functional marker and application thereof
  • Low amylose content gene wx-C39 functional marker and application thereof

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Embodiment Construction

[0025] Below in conjunction with accompanying drawing description, technical method of the present invention is described with specific embodiment:

[0026] (The embodiments of the present invention are described in detail below, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions. The embodiments described below by referring to the drawings are only used to explain the present invention, and cannot understood as a limitation of the invention.)

[0027]Chujing 39, Nipponbare, Huaidao 5, Nanjing 49, Nanjing 9108, 9311, IR36 and Huanghuazhan are all well-known and public materials, which can be provided free of charge by the Agricultural Germplasm Bank of Jiangsu Province.

[0028] The inventor's team screened a new material with low amylose content from the resource materials of local rice varieties in Yunnan. Through genetic analysis, it was found that the trait was a recessive trait with low sin...

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Abstract

The invention discloses a new soft rice waxy gene wx-C39 functional marker and application thereof, and belongs to the field of agricultural biological engineering. A molecular marker primer is WxC39-F:CGTCCCAGCTCGCCACCT and WxC39-R:CCACGCTGCACCGACCG, and if an amplification product is 173bp, it is indicated that wx-C39 homozygous genotype is carried, wherein the amylose content is about 5%; if 184bp is obtained through amplification, it is indicated that a homozygous genotype of Waxy is carried, wherein the amylose content is about 15%; if two bands are amplified simultaneously, it is indicated that a heterozygous genotype exists, wherein the amylose content is about 15%. The marker and a method can be used for filial generation genotype detection, and the soft rice breeding process can be accelerated conveniently. The method is high in accuracy, easy to operate, high in detection efficiency and low in cost, and has very high practical value.

Description

technical field [0001] The invention belongs to the field of agricultural bioengineering, and in particular relates to the functional marker of the gene wx-C39 with low amylose content and its application. Background technique [0002] Amylose content is an important indicator of quality traits of high-quality rice. Rice quality traits are complex comprehensive quantitative traits, mainly including four aspects: milling quality, appearance quality, cooking and eating quality and nutritional quality. The type and content of starch directly determine the cooking and eating quality of rice, among which the content of amylose is the most critical. Generally speaking, rice varieties with an amylose content of more than 20% have poor taste, while those with an amylose content of less than 15% to 20% have better taste. According to my country's high-quality rice quality index (GB / 717891-1999), the amylose content of indica rice is 15% to 24%, the amylose content of japonica rice ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王芳权杨杰范方军黄文兴王军周莉李文奇许扬王学辉张永华
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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