Application of a product detecting the expression level of a biomarker in carcinogenesis state indicating

A technology of biomarkers and expression levels, applied in the application field of articles for indicating cancerous state, to achieve the effect of complete form and high purity

Inactive Publication Date: 2018-09-18
谢丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] At present, there is no research on the mechanism and changes of exosomal LBP protein in malignant tumors in the prior art, and there is no report on using LBP as a marker for diagnosis and prediction of lung cancer metastasis

Method used

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  • Application of a product detecting the expression level of a biomarker in carcinogenesis state indicating
  • Application of a product detecting the expression level of a biomarker in carcinogenesis state indicating
  • Application of a product detecting the expression level of a biomarker in carcinogenesis state indicating

Examples

Experimental program
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Embodiment example

[0050] Implementation case: the application of LBP in the diagnosis of early non-small cell lung cancer and the prediction of metastasis

[0051] 1. Experimental design

[0052] The experiment was designed to collect serum samples from newly diagnosed patients with non-small cell lung cancer and healthy volunteers in Shandong Cancer Hospital, isolate and enrich exosomes and obtain exosome proteins. The expression of LBP was detected by ELISA and western blot, and the expression was statistically analyzed between healthy volunteers and early non-small cell lung cancer, between metastatic and non-metastatic non-small cell lung cancer, between lung adenocarcinoma and lung squamous cell carcinoma differences and analyze their diagnostic efficacy.

[0053] 2. Patients and samples involved in the experiment

[0054] 2 ml of serum samples were collected from 119 patients with early stage NSCLC in the Laboratory Department of Shandong Cancer Hospital, including 74 male patients and ...

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Abstract

The invention belongs to the field of medical and health, and particularly relates to application of a product detecting the expression level of a biomarker in carcinogenesis state indicating. The biomarker is LBP, a gene encoding LBP, or a polypeptide or polypeptide fragment synthesizing the LBP. It is proposed by the invention for the first time that the level of exosome protein LBP in serum ofpatients with non-small cell lung cancer is significantly higher than that of a healthy contrast group, and the level of exosome protein LBP of patients with metastatic non-small cell lung cancer is significantly higher than that of patients with non-metastatic non-small cell lung cancer, and therefore, the level of the exosome protein LBP in serum can be utilized for distinguishing patients withnon-small cell lung cancer from a contrast case, distinguishing metastatic non-small cell lung cancer, non-metastatic non-small cell lung cancer, lung adenocarcinoma and squamous-cell lung cancer.

Description

technical field [0001] The invention belongs to the field of medical and health care, and in particular relates to the application of a product for detecting the expression level of biomarkers in indicating the state of canceration. Background technique [0002] Non-small cell lung cancer (NSCLC) accounts for 80% of all lung cancers, about 75% of patients have distant metastasis at the time of diagnosis, and the 5-year survival rate is only 2%. Therefore, there is an urgent need for a more sensitive diagnostic marker to provide an objective basis for the early diagnosis, early treatment and prognosis of NSCLC with distant metastasis. [0003] Tumor cell-derived exosomes (Tomor-derived exosomes, TDEs) have been shown to promote cancer progression, and the intercellular interactions mediated by exosomes may play an important role in tumorigenesis and distant metastasis. Isolation of exosomes and their use in the clinic may be an alternative to invasive surgery for diagnosis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574C12Q1/6886
CPCG01N33/68C12Q1/6886C12Q2600/118G01N33/57423
Inventor 谢丽
Owner 谢丽
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