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Evaluation method and application of herbicide 2,4-d phytotoxicity

An evaluation method and herbicide technology, applied in the field of crop cultivation, can solve the problems of less research on evaluation methods and achieve the effect of ensuring accuracy

Active Publication Date: 2021-07-27
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] And in the research of prior art, only show that herbicide 2,4-D has certain harm to the growth of broad-leaved crops especially Schisandra chinensis, but for the evaluation method research of plant to herbicide 2,4-D resistance relatively few

Method used

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  • Evaluation method and application of herbicide 2,4-d phytotoxicity
  • Evaluation method and application of herbicide 2,4-d phytotoxicity
  • Evaluation method and application of herbicide 2,4-d phytotoxicity

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Embodiment approach

[0065] As a preferred embodiment of the present invention, in step (c), the damage of the herbicide 2,4-D to the photosynthetic mechanism of the plant can be judged according to the following conditions:

[0066] Compared with the control group, when the relative variable fluorescence induction kinetic curve of the treatment group appeared K point, and F v / F m Both PI and PI values ​​decreased, which means that the herbicide 2,4-D caused phytotoxicity to the photosynthetic mechanism of the plant.

[0067] Will F v / F m , PI value and K point are jointly used as the basis for judging the damage of the herbicide 2,4-D to the photosynthetic mechanism of the plant, ensuring the accuracy of the judgment result.

[0068] As a preferred embodiment of the present invention, the herbicide 2,4-D phytotoxicity evaluation method includes the following steps:

[0069] (a) select plants with consistent growth, and divide the plants into a treatment group that has sprayed herbicides and...

Embodiment 1

[0079] A kind of herbicide damage evaluation method provided by the present embodiment comprises the following steps:

[0080] (a) The annual grafted seedlings of Schisandra chinensis variety 'Yanhong' with consistent growth were selected, and Schisandra were divided into treatment group sprayed with herbicide 2,4-D and control group (CK) without herbicide 2,4-D sprayed Wherein, the treatment group that has sprayed herbicide 2,4-D is to dilute 2,4-D into 4000* (4000 times) dilution, adopt watering can to spray the dilution evenly on the lower surface of the blade, select The sixth leaf from the top of the botany is the test object;

[0081] At 24h, 48h and 72h (i.e. 1d, 2d and 3d) after the spraying treatment, the treatment group and the control group were dark-adapted for 20 minutes, and the Pocket-PEA instrument was used to light the saturated pulse light at 3000 μmol m -2 the s -1 Under the condition of PFD, the fast chlorophyll fluorescence induction kinetic curve was me...

Embodiment 2

[0085] A kind of herbicide phytotoxicity evaluation method that present embodiment provides, except that the treatment group that has sprayed herbicide 2,4-D in step (a) is that herbicide 2,4-D is diluted to 2000* (2000 times) Diluent, all the other steps are the same as in Example 1.

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Abstract

The invention provides a herbicide 2,4‑D phytotoxicity evaluation method and its application, and relates to the technical field of crop cultivation. The herbicide 2,4‑D phytotoxicity evaluation method is mainly to divide the plants into a treatment group and a control group, and after dark adaptation, measure their fast chlorophyll fluorescence induction kinetic curves respectively to obtain F v / F m and PI value, and conduct JIP-test analysis, observe whether the K point appears on the relative variable fluorescence induction kinetic curve, and set F v / F m , PI value and K point are jointly used as the basis for judging the damage of the herbicide 2,4‑D to the photosynthetic mechanism of the plant, ensuring the accuracy of the judgment result; at the same time, the phytotoxicity evaluation method can quickly, non-destructively and efficiently indicate The degree of damage to plants by spraying or drifting of herbicide 2,4‑D provides a reference method for studying the evaluation of plant resistance to herbicides. The invention also provides the application of the herbicide 2,4-D phytotoxicity evaluation method.

Description

technical field [0001] The invention relates to the technical field of crop cultivation, in particular to a herbicide 2,4-D phytotoxicity evaluation method and its application. Background technique [0002] In recent years, a variety of herbicides have been widely used in agriculture. While effectively and quickly controlling weeds in farmland, they have also improved production efficiency and liberated a large amount of labor. It is a huge task for agricultural production. progress. However, during the use of herbicides, the phytotoxicity of the surrounding adjacent crops is also a problem that cannot be ignored. For example, phenoxycarboxylic acids in herbicides can cause phytotoxicity to broad-leaved crops within 50m around during application, and herbicide 2,4-D can cause phytotoxicity to broad-leaved crops 500m downwind. [0003] Specifically, taking the medicinal economic plant Schisandra chinensis as an example, the herbicide 2,4-D will cause harm to Schisandra chin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 许培磊艾军王振兴张宝香孙丹范书田李昌禹
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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