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A method for detecting circulating tumor cells based on surface-enhanced Raman spectroscopy probe

A surface-enhanced Raman and tumor cell technology, applied in the field of spectroscopy, achieves low cost, good sensitivity, and low cytotoxicity

Active Publication Date: 2021-01-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestically, there are few patents or other documents on the use of SERS probe technology for CTCs detection, and there is no report on the SERS probe targeting LHRH. This field still needs continuous research and progress to provide more clinical evidence. testing method

Method used

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  • A method for detecting circulating tumor cells based on surface-enhanced Raman spectroscopy probe
  • A method for detecting circulating tumor cells based on surface-enhanced Raman spectroscopy probe
  • A method for detecting circulating tumor cells based on surface-enhanced Raman spectroscopy probe

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Experimental program
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Embodiment 1

[0039] Step 1: Preparation of SERS probes

[0040] 0.01% (W / W) of HAuCl 4 Heat the solution to a slight boil, slowly add 1ml of 1% (W / W) C 6 h 5 Na 3 o 7 2H 2 O solution, after heating for 10 minutes, 45nm gold particles can be obtained; 1ml 45nm gold particle solution and 50ul 1mM pMBA are mixed and shaken for 5 minutes, and then centrifuged at 5000rpm to resuspend, so that pMBA can be combined with gold nanoparticles to obtain Au-pMBA; 20ml 20mg / ml Bovine serum albumin (BSA) and 260ul 1M sodium borohydride (NaBH 4 ) mixed reaction for 3 hours, 60 ℃ constant temperature water bath to exhaust the excess hydrogen to obtain rBSA, after cooling, take 5ml rBSA and add 30ul 10mM 1-(3-dimethylaminopropyl) 3-ethylcarbodiimide hydrochloride (EDC HCl) and N-hydroxysuccinimide (NHS) to activate the carboxyl group, after 30 minutes of reaction, use a 3KDa ultrafiltration centrifuge tube at 5000rpm to centrifuge for 10 minutes to remove excess EDC / NHS, then add 50ul 2.5mg / ml LHRH so...

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Abstract

The invention relates to the technical field of spectra, in particular to a circulating tumor cell detection method based on a surface-enhanced Raman spectroscopic probe. The method includes the steps: building the surface-enhanced Raman spectroscopic probe taking luteinizing hormone-releasing hormone as a targeting molecule; using the surface-enhanced Raman spectroscopic probe for circulating tumor cell detection. The surface-enhanced Raman spectroscopic probe is rapider, more convenient and lower in cost when being used for circulating tumor cell detection, has enough specificity and flexibility for circulating tumor cell detection and can provide a feasible strategy for clinical practice.

Description

technical field [0001] The invention relates to the field of spectroscopic technology, in particular to a method for detecting circulating tumor cells based on a surface-enhanced Raman spectroscopic probe. Background technique [0002] Circulating tumor cells (CTCs) refer to the micro tumor cells that are released from the tumor in situ into the blood, transfer through the blood and colonize other parts of the body to cause tumor metastasis. These cells play a role in the process of malignant tumor metastasis. Therefore, it is an important cause of death caused by malignant tumors. Studies have shown that the level of CTCs in patients with breast cancer, lung cancer, pancreatic cancer, prostate cancer, and ovarian cancer is closely related to their progression-free survival and overall survival. Therefore, early, simple, and rapid detection of CTCs is important for improving the prognosis of cancer patients. very important. However, it is reported that the lowest content o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陆峰唐瑞胡然崔晓林柳艳柴逸峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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