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Application of a mutant zebrafish in the preparation of an animal model of variable immunodeficiency disease

A technology for immunodeficiency diseases and animal models, which is applied in the application field of animal models, can solve unclear problems, and achieve the effects of convenient administration, low cost, and convenient and easy breeding

Inactive Publication Date: 2020-06-19
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Therefore, the runx1 gene plays an irreplaceable important role in maintaining the normal development of hematopoietic cells, but many mechanisms of the runx1 gene on the development and maturation of B lymphocytes are still unclear, and further research is needed

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  • Application of a mutant zebrafish in the preparation of an animal model of variable immunodeficiency disease
  • Application of a mutant zebrafish in the preparation of an animal model of variable immunodeficiency disease
  • Application of a mutant zebrafish in the preparation of an animal model of variable immunodeficiency disease

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Embodiment 1

[0042] 1. Materials and methods:

[0043] (1) Zebrafish farming

[0044] Zebrafish were cultured as described in the literature (Westerfield M: The zebrafish: guide for the laboratory use of zebrafish (Brachdanio rerio). Edition by Eugene, OR, M. Westerfield, 1993).

[0045] The following strains are used in the present invention: AB wild-type zebrafish, runx1 W84X / W84X Mutant zebrafish, Tg(Cau.Ighv-ighm:EGFP) transgenic zebrafish, Tg(rag2:DsRED) transgenic zebrafish, Tg(lck:loxp-DsRED-loxp-GFP) transgenic zebrafish. Among them, each transgenic zebrafish line was combined with runx1 W84X / W84X Crossbreeding of mutant zebrafish followed by selfing to obtain runx1 with different transgenic backgrounds W84X / W84X Mutant zebrafish and wild-type zebrafish (runx1 + / + control fish), such as Tg(Cau.Ighv-ighm:EGFP); runx1 + / + and Tg(Cau.Ighv-ighm:EGFP); runx1 W84X / W84X , Tg(rag2:DsRED); runx + / + and Tg(rag2:DsRED); runx1 W84X / W84X , Tg(lck:loxp-DsRED-loxp-GFP); runx + / + and Tg...

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Abstract

The invention discloses application of mutant zebra fish in preparation of an animal model of a common variable immunodeficiency disease (CVID). The mutant zebra fish is runx1W84X / W84X mutant zebrafish. It is found for the first time in the invention that runx1 mutation of the zebrafish is associated with B lymphocyte development, the zebrafish runx1W84X / W84X mutant has the symptoms of the CVID similar to humans, and it is proved that the runx1W84X / W84X mutant zebrafish B has a lymphocyte immune response function defect. In addition, the human CVID disease model with B lymphocyte development disorder, IgM and IgZ rearrangement defects and T cell development unaffected or slightly affected is established for the first time. The zebrafish model can become a new carrier for studying the CVIDand drug screening.

Description

technical field [0001] The invention belongs to the field of biological technology, and particularly relates to the application of a mutant zebrafish in preparing an animal model of a variable immunodeficiency disease. Background technique [0002] The RUNX protein family is a family of transcription factors with highly conserved protein sequences, including a DNA binding domain and a protein interaction domain. Members of the RUNX family arose very early in animal evolution and maintain extensive structural similarities. Runx-related transcription factor 1 (Runt-related transcription factor l, Runxl, also known as AML1 / CBFA2), is one of the important members of the runx family. During ontogeny, runx1 is expressed in tissues and organs related to the initiation of HSCs, is one of the main regulators of gene expression in hematopoietic cells, and is also a common target site for various chromosomal translocations in human leukemia. The proliferation and differentiation of h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735C12N15/06
CPCA01K67/0275A01K2227/40A01K2267/0393
Inventor 张文清黄志斌迟雅丽陈琪徐进
Owner SOUTH CHINA UNIV OF TECH
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