Tissue preserving fluid

A technology for preserving liquid and tissue, which is applied in the field of biomedicine, can solve the problems of reduced success rate of PDX tumor model building, contamination of tumor cells by fibroblasts, and application limitations of primary tumor cells, so as to increase the possibility of curing chordoma, Tumor formation time is shortened, which is beneficial to clinical application

Active Publication Date: 2018-11-02
HUNAN SAIAOWEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Serum can easily differentiate chordoma tumor cells and reduce the ability of malignant proliferation
Therefore, it will lead to the differentiation of tumor samples and the decrease of activity, resulting in a decrease in the success rate of PDX tumor model building, increasing the cost

Method used

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Experimental program
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Embodiment Construction

[0033] 1. Configuration of the tissue preservation solution of the present invention

[0034] The method for preparing tissue preservation solution of the present invention:

[0035] Take a 50ml sterile centrifuge tube

[0036]

[0037] Take out 25ml of tissue preservation solution and put it into another 50ml sterile centrifuge tube. Under sterile conditions, fresh typical chordoma tumor tissues were taken out, put into the above-mentioned centrifuge tubes, and sent to a sterile cell culture room for primary culture.

[0038] 2. Primary culture

[0039] (1) Select a fresh chordoma sample and place it in a 10cm 2 in a sterile petri dish

[0040] (2) Rinse with sterile PBS buffer

[0041] (3) After rinsing, use a blade to cut out a small piece of the sample with a size of 0.5cm*0.5cm, move it to the lid of the petri dish, add 1ml of tissue preservation solution to prevent the tissue cells from drying out

[0042] (4) Cut the selected small sample into small pieces with...

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Abstract

The invention discloses tissue preserving fluid. Each 50mL of the tissue preserving fluid is prepared from the following components: 50ml of DMEM (Dulbecco's Modified Eagle Medium)/F12, 90 to 110mu Lof bFGF (Basic Fibroblast Growth Factor), 240 to 260mu L of N2, 480 to 520mu L of B27, 40 to 60mu L of Y-27632, 400 to 500ng of BDNF (Brain Derived Neurotrophic Factor) and 400 to 500ng of NT3. By applying the tissue preserving fluid disclosed by the invention, the activity of fresh tumor cells can be kept for relatively long time, so that the efficiency of obtaining a PDX (Patient-Derived tumor Xenograft) model is improved. Compared with convectional preserving fluid, the PDX model utilizing the preserving fluid has the advantage that the tumor formation rate is greatly increased and the tumor utilization rate is greatly improved; the tumor formation and primary cell culture time is remarkably shortened. Moreover, the method disclosed by the invention can be used for enhancing the activity of a chordoma sample, so that the cost of a chordoma PDX model experiment is reduced; precision medical treatment can be carried out on tumors of more patients through the PDX model, so that the possibility of healing chordoma is improved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a fast and efficient optimization method for preparing a tumor transplantation model derived from a patient's chordoma tissue, and specifically relates to a tumor transplantation (Patient- derived xenograft (PDX) model and the method of obtaining high-purity primary tumor cells. Background technique [0002] Chordomas are locally invasive or malignant neoplasms arising from embryonic residual or lost notochord tissue. It can occur anywhere along the midline of the spine, but is most common on the rostral side of the clivus and the sacrum. Chordoma is very destructive in local area, it will endanger the human body because the tumor continues to grow, and it is very easy to relapse after surgery, so it is still a malignant tumor. At present, there is no good drug that can completely cure the tumor. [0003] The PDX tumor model is a new generation of tumor model established b...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 刘思文蔡娅段荣燕蔡娜
Owner HUNAN SAIAOWEI BIOTECH CO LTD
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