Method for promoting growth of orchidaceae through Ceratobasidium sp.

Abstract

The invention discloses a method for promoting growth of orchidaceae through Ceratobasidium sp.. A symbiotic relationship is established between a strain of ceratobasidium and protocorm / rhizomes and seedlings of the orchidaceae to promote growth of the orchidaceae, wherein the strain of ceratobasidium is Ceratobasidium sp. of ceratobasidiaceae, has the Latin name of Ceratobasidium sp., the GenBankregistration number of MG669582 and the biological preservation number of CGMCC No.15373. Through the promoting function of the Ceratobasidium sp. on growth of the orchidaceae, a foundation is laid for application of the Ceratobasidium sp. in efficient breeding of orchidaceae and resource protection of wild orchidaceae.

Description

technical field

[0001] The present invention relates to a kind of cultivation method of Orchidaceae, in particular to a method for promoting the growth of Orchidaceae by a fungus of the genus Carobasidium. Background technique

[0002] Orchidaceae (Orchidaceae) belongs to the class Monocots of the angiosperm phylum, and there are about 700 genera and more than 35,000 species distributed in the world (Dressler, 1993; Cribb, 2003), and its number of species ranks second only to Compositae. Branch (Lang Kaiyong, 1994). It is beautiful in shape, bright in color, and has great ornamental and medicinal value.

[0003] Orchidaceae has two notable characteristics: one, the embryo of orchidaceae seed is only made up of dozens of cells, and differentiation is incomplete, and it is difficult to germinate naturally. 2. Orchidaceae are almost all mycorrhizal plants. In the natural growth state, no matter green leaf orchid or non-green leaf orchid, in its life history—the stage of seed ...

Examples

specific Embodiment

[0019] 1. Test material

[0020] (1) Plant materials: protocorms and seedlings of Cymbidium mannii, rhizomes and seedlings of Cymbidium ensifolium, and protocorms and seedlings of Dendrobium officinale.

[0021] (2) Fungal material: A strain of Ceratobasidium sp. was isolated and obtained in our laboratory.

[0022] 2. Test method (symbiosis test between protocorms / seedlings of Orchidaceae and fungi of the genus Carobasidium)

[0023] (1) Configuration of culture medium

[0024] The configuration of symbiotic medium (oat medium OMA): take oatmeal and grind it into powder, take three gradients of oat flour respectively, and prepare 0.5g / L, 1.0g / L, 2.0g / L, 3.0g / L OMA solution. Boil in deionized water for 1 hour, filter, add 8.0 g of agar, add deionized water to the filtrate to 1000 mL, and sterilize at high temperature.

[0025] The configuration of the fungal culture medium: 200g peeled potatoes, cut into small pieces of 0.5-1cm square, add 800mL of deionized water and cook...

technical field

[0001] The present invention relates to a kind of cultivation method of Orchidaceae, in particular to a method for promoting the growth of Orchidaceae by a fungus of the genus Carobasidium. Background technique

[0002] Orchidaceae (Orchidaceae) belongs to the class Monocots of the angiosperm phylum, and there are about 700 genera and more than 35,000 species distributed in the world (Dressler, 1993; Cribb, 2003), and its number of species ranks second only to Compositae. Branch (Lang Kaiyong, 1994). It is beautiful in shape, bright in color, and has great ornamental and medicinal value.

[0003] Orchidaceae has two notable characteristics: one, the embryo of orchidaceae seed is only made up of dozens of cells, and differentiation is incomplete, and it is difficult to germinate naturally. 2. Orchidaceae are almost all mycorrhizal plants. In the natural growth state, no matter green leaf orchid or non-green leaf orchid, in its life history—the stage of seed ...

Examples

specific Embodiment

[0019] 1. Test material

[0020] (1) Plant materials: protocorms and seedlings of Cymbidium mannii, rhizomes and seedlings of Cymbidium ensifolium, and protocorms and seedlings of Dendrobium officinale.

[0021] (2) Fungal material: A strain of Ceratobasidium sp. was isolated and obtained in our laboratory.

[0022] 2. Test method (symbiosis test between protocorms / seedlings of Orchidaceae and fungi of the genus Carobasidium)

[0023] (1) Configuration of culture medium

[0024] The configuration of symbiotic medium (oat medium OMA): take oatmeal and grind it into powder, take three gradients of oat flour respectively, and prepare 0.5g / L, 1.0g / L, 2.0g / L, 3.0g / L OMA solution. Boil in deionized water for 1 hour, filter, add 8.0 g of agar, add deionized water to the filtrate to 1000 mL, and sterilize at high temperature.

[0025] The configuration of the fungal culture medium: 200g peeled potatoes, cut into small pieces of 0.5-1cm square, add 800mL of deionized water and cook...