MSP1 gene based cultivation method for transgenic rice sterile line

Abstract

The application discloses an MSP1 gene based cultivation method for a transgenic rice sterile line, which comprises the steps as follows: A, acquisition of an mPlum gene cassette; B, acquisition of arice MSP1 gene cassette; C, amplification of a wheat lethal gene K1; D, amplification of a wheat pollen specific promoter Pg47; and E, connection of each gene expression element. The step E of connection of each gene expression element further comprises: a first step of connecting the mPlum gene expression element to a pCAMBIA1301 vector; and a second step of introducing a complete rice MSP1 geneinto the pCAMBIA1301 vector in which the mPlum gene expression element is introduced in the first step. According to the invention, a F1-generation heterozygote follows the Mendel segregation law in the self fruitfulness process, and generated progenies not only contain heterozygotes which can keep a triple linked gene, but also contain the sterile line without fertility.

Description

technical field [0001] The invention relates to the technical field of molecular biology nucleic acid chemistry, in particular to a method for preparing a genetically engineered sterile line of rice. Background technique [0002] Rice is the staple food of nearly half of the world's population. In addition to being edible, it can also be used for wine making, sugar refining and as an industrial raw material. Humans have a great demand for rice. Years of production practice have shown that hybrid rice can generally increase yield by more than 20% compared with conventional rice, so hybrid rice shows great potential for increasing yield. [0003] The development of hybrid rice depends on the cultivation of male sterile lines of hybrid rice. Research on hybrid rice in my country began in the 1960s, and it began to be planted on a large scale in the 1970s. The first-generation hybrid rice is a three-line method using the nuclear-plasma interaction male sterile line as a geneti...

AI Technical Summary

Problems solved by technology

In the three-line method, the available parents with good shape are limited, the combination is not free, and the frequency of wild-lost maintenance lines is low

[0004] The genetic difference between the existing sterile lines in production is small, the fertility stability of hybrids is not enough, and the ability to resist adversity is poor

However, in the "two-line method", the fertility of the photothermosensitive male sterile line is controlled by the external temperature, which may easily lead to the self-fertilization of the photothermosensitive male sterile line and the failure of seed production.

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