Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition and its use in preparation of anti-inflammatory drug

A composition and drug technology, applied in the directions of anti-inflammatory agents, drug combinations, antipyretics, etc., can solve the problems of large toxic and side effects, high price, large dosage, etc., and achieve excellent drug efficacy, low price, and dosage. low effect

Active Publication Date: 2018-12-07
GUANGDONG UNIV OF TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the defects of large dosage, large toxic and side effects and high price described in the above-mentioned prior art, the present invention provides a composition, which uses silibinin and dibenzoylmethane in combination, anti-inflammatory The effect is good, the drug effect is obviously better than that of single drug, and the dosage is low, effectively reducing the side effects of single drug, and the price is relatively cheap

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition and its use in preparation of anti-inflammatory drug
  • Composition and its use in preparation of anti-inflammatory drug
  • Composition and its use in preparation of anti-inflammatory drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) MTT experiment

[0037] MTT assay was used to measure the effects of different concentrations of silibinin, dibenzoylmethane and the combination of the two on the growth of RAW264.7 cells. The specific embodiments are as follows:

[0038] RAW264.7 cells were digested from the culture flask with trypsin, centrifuged for 5 minutes, added to DMEM medium containing 10% FBS to make a cell suspension, and seeded at a concentration of 5×10 4 Inoculated in 96-well plates (100 μL / well) at the concentration of cells / mL and placed in 5% CO 2 , and cultured at 37°C for 24 hours. Set the concentrations of silibinin and dibenzoylmethane to 80µM, 40µM, 20µM, 10µM, 5µM and 2.5µM, and set 6 replicate wells for each concentration. 5% CO 2 After culturing at 37°C for 24 hours, carefully aspirate the culture medium, and add 150 µL of MTT solution (0.5 mg / mL) prepared in serum-free medium to each well; after continuing to cultivate for 4 hours, carefully aspirate the culture medium i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composition and its use in the preparation of an anti-inflammatory drug. The composition comprises silybin and dibenzoyl methane. Through combination of silybin and dibenzoylmethane, good inhibition effects on a lipopolysaccharide-induced macrophage inflammatory model are obtained and drug effects are obviously superior to that of the single medical ingredient. The composition has a low dosage in inflammatory treatment, significantly reduces the concentration of the single drug, reduces the toxic or side effects of the individual drug, reduces the damage to the humanbody and has a low price.

Description

technical field [0001] The present invention relates to the technical field of anti-inflammatory drugs, more specifically, to a composition and its application in the preparation of anti-inflammatory drugs. Background technique [0002] Inflammation is a complex biological response of body tissues to harmful stimuli such as pathogens, damaged cells or irritants, and is a protective response involving immune cells, blood vessels and molecular mediators. The function of inflammation is to remove dead cells and tissues damaged from the original injury and the inflammatory process, and to initiate tissue repair. [0003] In the inflammatory process, on the one hand, the damage factors directly and indirectly cause tissue and cell destruction, and on the other hand, the damage factors are diluted, killed and surrounded by the inflammatory hyperemia and exudative reaction. At the same time, the damaged tissue can be repaired and healed through the regeneration of parenchyma and i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/357A61K31/12A61P29/00
CPCA61K31/12A61K31/357A61P29/00A61K2300/00
Inventor 黄泽彬郑希张蓝月刘俊磊王华威吴晓凤谢玲娜谢荣韬陈颖欣黄华容张焜陈柏润张佳敏陈星宇
Owner GUANGDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com