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ADAMTS6 gene and shRNA sequences thereof and application in antihuman lung cancer

A technology of DNA sequence, lung cancer, applied in the field of biomedicine

Inactive Publication Date: 2018-12-07
JIANGSU PROVINCE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on ADANTS6 in tumors. Studies have shown that ADAMTS6 can inhibit the progression of breast cancer through the ERK signaling pathway and serve as a prognostic marker for human breast cancer [8]

Method used

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  • ADAMTS6 gene and shRNA sequences thereof and application in antihuman lung cancer
  • ADAMTS6 gene and shRNA sequences thereof and application in antihuman lung cancer
  • ADAMTS6 gene and shRNA sequences thereof and application in antihuman lung cancer

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Experimental program
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Effect test

Embodiment 1

[0042] 1. Interference plasmid preparation and sequencing:

[0043] Search the ADAMTS6 gene protein coding sequence through the NCBI website, as shown in SEQ ID NO: 1, design the expression DNA sequence of the shRNA interference fragment of the ADAMTS6 gene:

[0044] shADMATS sense strand: 5′-GATCCCCTGACTTATCTTGAACACTATTCAAGAGATAGTGTTCAAGATAAGTCAGGTTTTTTG-3′;

[0045] shADMATS antisense strand: 5'-AATTCAAAAAACCTGACTTATCTTGAACACTATCTCTTGAATAGTGTTCAAGATAAGTCAGGG-3'.

[0046] And perform interference plasmid cloning (LV3-GFP vector was purchased from Shanghai Gemma Co., Ltd.), and the successfully cloned LV3-GFP-shADAMTS6 plasmid was sent to a sequencing company for sequencing. 2. Knockdown the plasmid for sequencing, such as figure 1 As shown, the sequencing results showed that the knockdown fragment had been successfully inserted into the LV3-GFP vector.

[0047] 2. Observation of the fluorescent density of the stable knockdown of the interference plasmid:

[0048] The const...

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Abstract

The invention discloses a gene ADAMTS6 for inhibiting anti-human lung cancer and shRNA thereof. The shRNA comprises a sequence shown as SEQ ID NO: 2 or SEQ ID NO: 3. The specific shRNA sequence designed in the invention is capable of effectively realizing targeted inhibition of expression of the gene ADAMTS6, and high-efficiency and convenient means can be provided for gene therapy of the lung cancer in future.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to ADAMTS6 gene and its shRNA sequence and its application in anti-human lung cancer. Background technique [0002] Lung cancer has become one of the most common malignant tumors in our country, threatening the health of urban residents in our country with its high mortality rate. With the rapid development of population, economy and society, the incidence of lung cancer is also increasing rapidly. Many studies have pointed out that lung cancer in my country has become a serious disease that endangers public health, and there is an urgent need for practical and effective strategies to control its damage. [1] . [0003] Matrix metalloproteinases (a disintegrin and metalloproteinase with thrombospondin motifs, ADAMTS) belong to the family of multi-domain extracellular proteases, which mainly include 19 members according to different functions, and are mainly involved in the secretion of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/7105A61K31/711A61K48/00A61P35/00
CPCA61K31/7105A61K31/711A61P35/00C12N15/1135C12N2310/122
Inventor 束永前刘捷帅优
Owner JIANGSU PROVINCE HOSPITAL
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