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A Coplanar Parallel Detection Method for Multilayer Signals Based on Gradient Reflection

A layer signal and signal technology, which is applied in the field of coplanar parallel detection of multi-layer signals based on gradient reflection, can solve the problems of prolonged acquisition time and inability to obtain axial signals of microscopic imaging systems, shorten the detection time interval, and improve three-dimensional Imaging speed, the effect of strong applicability

Active Publication Date: 2021-03-02
HUST SUZHOU INST FOR BRAINMATICS
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Problems solved by technology

However, when the volume of the imaging sample needs to be further increased, the acquisition time of the microscopic imaging system will be greatly extended
In addition, when detecting the instantaneous change process of living biological signals, the sequential imaging method cannot obtain a large volume of axial signals before the signal disappears

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  • A Coplanar Parallel Detection Method for Multilayer Signals Based on Gradient Reflection
  • A Coplanar Parallel Detection Method for Multilayer Signals Based on Gradient Reflection
  • A Coplanar Parallel Detection Method for Multilayer Signals Based on Gradient Reflection

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[0016] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0017] For ease of description, an example of a multi-layer signal satisfying the condition is given, and the applicable signal of the present invention is not limited thereto. Figure 1 ~ Figure 3 As shown, the multi-layer signal is a three-strip signal with axial and lateral intervals at the same time, where the Z-axis direction is the axial direction, the XY plane is the imaging plane parallel to the objective lens, and the distances of the signals on the imaging plane are respectively for Δx 1 and Δx 2 , the signal axial intervals are Δz 1 and Δz 2 .

[0018] The present invention provides...

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Abstract

The invention belongs to the technical field of optical signal detection, and discloses a multi-layer signal coplanar parallel detection method based on gradient reflection, which includes the following steps: Step 1, generating a group of multi-layer signals, and the adjacent layer signals in the multi-layer signals have axes In step 2, the multi-layer signals are compensated by axial reflection to eliminate the axial displacement difference; in step 3, the compensated multi-layer signals are coplanar and clearly imaged on the same detection surface. The invention shortens the detection time interval when the detector acquires different axial positions of the sample.

Description

technical field [0001] The invention relates to the technical field of optical signal detection, in particular to a multi-layer signal coplanar parallel detection method based on gradient reflection. Background technique [0002] In the field of bioimaging, shallow imaging of surfaces is often used when imaging larger samples. The piezoelectric scanner drives the objective lens to move axially or the three-dimensional translation stage drives the sample to produce mechanical cutting to remove the imaged sample part. In this way, the detector sequentially detects the signals of different axial positions of the sample. However, when the volume of the imaging sample needs to be further increased, the acquisition time of the microscopic imaging system will be greatly extended. In addition, when detecting the instantaneous change process of living biological signals, the sequential imaging method cannot obtain a large volume of axial signals before the signal disappears. There...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 龚辉袁菁余亚兰陈键伟骆清铭
Owner HUST SUZHOU INST FOR BRAINMATICS
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