Kiwifruit micro-grafting method and kiwifruit seedling growing method

A kiwifruit micro-grafting technology, which is applied in grafting, horticultural methods, botanical equipment and methods, etc., can solve problems such as unstable traits, low survival rate, and long time for kiwifruit cultivation and reproduction, so as to improve the survival rate, reduce diseases, Achieve the effect of virus removal

Active Publication Date: 2018-12-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kiwifruit micro-grafting method and a kiwifruit seedling raising method to solve the problems of long breeding time, unstable character and low survival rate of the existing kiwifruit

Method used

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  • Kiwifruit micro-grafting method and kiwifruit seedling growing method
  • Kiwifruit micro-grafting method and kiwifruit seedling growing method
  • Kiwifruit micro-grafting method and kiwifruit seedling growing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The kiwi fruit micro-grafting method of the present embodiment comprises:

[0030] (1) Obtain tissue culture seedlings:

[0031] (11) In April, take 1.5 cm of the young stem section of 'Miliang No. 1' kiwifruit, and in March, take 1 cm of the new stem section of 'Hongyang' and 'Hongyang' kiwifruit, and then carry out sterilization treatment respectively; Sterilization method: use 70v% alcohol solution for 25 seconds, then use 0.05v% mercury solution for 6 minutes;

[0032] (12) The kiwifruit stems of 'Miliang 1' were cultured in the primary medium of MS+0.5mg / L 6-BA+0.5mg / L NAA+0.5mg / L 2,4-D, and the 'Hongyang 'Kiwifruit stems were cultured in the primary medium of MS+1.0mg / L 6-BA+0.1mg / L NAA to induce callus and then differentiate into young shoots;

[0033] (13) Put the kiwifruit stem section of 'Miliang No. 1' after primary culture in MS+2.0mg / L 6-BA+0.1mg / L NAA+0.5mg / L GA 3 Cultured and multiplied in the subculture medium of the first generation, the stem segment...

Embodiment 2

[0044] The kiwi fruit micro-grafting method of the present embodiment comprises:

[0045] (1) Obtain tissue culture seedlings:

[0046] (11) In April, take 2.5 cm of the young stem section of 'Miliang No. 1' kiwifruit in the same year, and in March, take the 1.5 cm section of the new shoot of 'Hongyang' kiwifruit in March, and then carry out sterilization treatment respectively; sterilization method It is: after 15s treatment with 80v% alcohol solution, 4min treatment with 0.15v% mercuric solution;

[0047] (12) The kiwifruit stems of 'Miliang 1' were cultured in the primary medium of MS+0.5mg / L 6-BA+0.5mg / L NAA+0.5mg / L 2,4-D, and the 'Hongyang 'Kiwifruit stems were cultured in the primary medium of MS+1.0mg / L 6-BA+0.1mg / L NAA to induce callus and then differentiate into young shoots;

[0048] (13) Put the kiwifruit stem section of 'Miliang No. 1' after primary culture in MS+2.0mg / L 6-BA+0.1mg / L NAA+0.5mg / L GA 3 Cultured and multiplied in the subculture medium of the first ...

Embodiment 3

[0059] The kiwi fruit micro-grafting method of the present embodiment comprises:

[0060] (1) Obtain tissue culture seedlings:

[0061] (11) In April, take 2 cm of the young stem section of 'Miliang No. 1' kiwifruit in the same year, and in March, take the 1.2 cm section of the new shoot of 'Hongyang' kiwifruit in March, and then carry out sterilization treatment respectively; the sterilization method is as follows: : Treat with 75v% alcohol solution for 20s, then treat with 0.1v% mercuric solution for 5min;

[0062] (12) The kiwifruit stems of 'Miliang 1' were cultured in the primary medium of MS+0.5mg / L 6-BA+0.5mg / L NAA+0.5mg / L 2,4-D, and the 'Hongyang 'Kiwifruit stems were cultured in the primary medium of MS+1.0mg / L 6-BA+0.1mg / L NAA to induce callus and then differentiate into young shoots;

[0063] (13) Put the kiwifruit stem section of 'Miliang No. 1' after primary culture in MS+2.0mg / L 6-BA+0.1mg / L NAA+0.5mg / L GA 3 Cultured and multiplied in the subculture medium of ...

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Abstract

The invention discloses a kiwifruit micro-grafting method and a kiwifruit seedling growing method, and belongs to the technical field of plant grafting. Two kiwifruits which are 'Hongyang' and 'Miliang 1' kiwifruits are adopted as grafted objects, an in-vitro rapid propagation system for grafting the two kiwifruits is established, explants used for grafting, an initial culture medium, a subculturemedium, a rooting medium, and the grafting method and a grafting medium are systematically studied to determine the in-vitro rapid propagation system adopting 'Miliang 1' kiwifruit tissue culture seedlings as rootstocks and the 'Hongyang' kiwifruit as cion, so other parameters facilitating the improvement of the induction rate, the tissue proliferating and rooting number and the grafted seedlingsurvival rate are determined, the propagation time is shortened, and the excellent traits of two kiwifruit varieties are reserved; and the control of environmental parameters of grafting greatly improves the survival rate of the grafted seedlings and improves the efficiency of existing kiwifruit production systems.

Description

technical field [0001] The invention relates to the technical field of plant grafting, in particular to a kiwifruit micro-grafting method and a kiwifruit seedling raising method. Background technique [0002] Kiwifruit is a perennial deciduous vine fruit tree of the Actinidiaceae Actinidia family, also known as Maoli, Kiwi, Carambola, etc. It has very prominent nutritional functions, V C content is very rich. It is native to my country and mainly produced in Asia. In my country, it is mainly distributed in warm temperate and subtropical mountainous areas. The Yangtze River Basin and its south are the main production areas. Among them, Shaanxi, Sichuan, Henan, Hubei, Guizhou and other places are distributed. [0003] There are 66 kinds of kiwifruit varieties in the world, and 62 kinds in China. After the first kiwifruit scientific research cooperation meeting in 1978, my country's kiwifruit industry developed rapidly. At present, the total production area of ​​kiwifruit in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/30
CPCA01G2/30A01H4/001A01H4/008
Inventor 张勇孔令灵汤浩茹陈清罗娅孙勃王小蓉张皓月程丽娟
Owner SICHUAN AGRI UNIV
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