Method for effectively improving cut-out rate of pathological parathyroid tissue sections of experimental animals

A parathyroid and experimental animal technology, applied in the preparation of test samples, sampling devices, etc., can solve the problems of parathyroid tissue loss, affecting the quality of the test, and inability to cut out the parathyroid tissue, so as to ensure accuracy. , Improve the test quality, improve the effect of cutting out rate

CN109115543AActive Publication Date: 2019-01-01苏州华测生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-01-01

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Abstract

The invention discloses a method for effectively improving the cut-out rate of pathological parathyroid tissue sections of experimental animals. The method comprises the following steps: euthanasia ofan experimental animal; dissection; removal of a parathyroid together with a thyroid; fixation via neutral formalin with a concentration of 10%; marking with a hematorylin solution; dehydration; paraffin embedding; slicing; and dyeing. Through comparative study of the method provided by the invention and traditional preparation methods, the novel section preparation methods increases the parathyroid cut-out rate of the animal to 100% from conventional 50 to 70%; and the method is simple, easy to master, extensively applicable to preparation of parathyroid tissue sections of experimental animals, and capable of effectively preventing the situation that the parathyroid is lost or cannot be cut out during slicing.
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Description

technical field

[0001] The invention relates to a method for effectively improving the cut-out rate of pathological slices of parathyroid tissue of experimental animals, belonging to the field of new methods of pathological technology. Background technique

[0002] In the single-dose and repeated-dose toxicity tests of preclinical safety evaluation of new drugs, the parathyroid gland is the tissue organ that is required to be inspected according to the guidelines issued by the State Drug Administration. Because the parathyroid gland is too small and its location is irregular, it is difficult to cut it out by traditional methods, resulting in the loss of observation records of parathyroid gland slices in some animals. Especially when the target organ of some drugs is the parathyroid gland, the correct judgment of the test results may be affected due to the low rate of parathyroidectomy, which seriously affects the quality of the test and the integrity of the data.

[0003] T...

Examples

Embodiment 1

[0028] Embodiment 1 hematoxylin staining method

[0029] The fixed thyroid gland was wiped dry with dust-free paper to check the location of the parathyroid glands. The tip of the toothpick was blunted and dipped in hematoxylin solution for staining to stain the parathyroid glands. After the hematoxylin solution was dry, it was dehydrated together with other tissues. When embedding, embed the parts with hematoxylin color on both sides at the same horizontal position. When slicing, the tissue should be roughly trimmed, and carefully observed when it is close to the site marked by hematoxylin, and sliced ​​after the parathyroid glands are trimmed out.

Embodiment 2

[0030] Example 2 Comparative study of hematoxylin-labeled parathyroid glands and traditional film-making methods

[0031] Research method: Thyroid and parathyroid gland tissues of 250 rats were randomly prepared by 5 methods. The production process of the conventional method is as follows: the parathyroid glands together with the thyroid are taken out → 10% neutral formalin fixation → dehydration → paraffin embedding → sectioning → staining. Hematoxylin solution 1-4 labeling method The production process is as follows: take out the parathyroid gland together with the thyroid gland → 10% neutral formalin fixation → label the parathyroid gland with hematoxylin solution 1 → dehydration → paraffin embedding → sectioning → staining; Parathyroid glands were taken out together with the thyroid → 10% neutral formalin fixation → hematoxylin solution 2 labeled parathyroid glands → dehydration → paraffin embedding → sectioning → staining; parathyroid glands were taken out together with t...