Kit for detecting cervical cancer and precancerous lesions thereof and application thereof

A technology for precancerous lesions and cervical cancer, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as low sensitivity and specificity, high trauma, and limited ability to diagnose early patients

Active Publication Date: 2019-01-11
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have certain value in the diagnosis of early and middle-advanced cervical cancer patients, their sensitivity and specificity are not high, and they are more invasive, and their diagnostic ability for early-stage patients is limited.

Method used

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  • Kit for detecting cervical cancer and precancerous lesions thereof and application thereof
  • Kit for detecting cervical cancer and precancerous lesions thereof and application thereof
  • Kit for detecting cervical cancer and precancerous lesions thereof and application thereof

Examples

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Embodiment 1

[0080] Example 1. Development of plasma miRNA detection kit for cervical cancer and its precancerous lesions

[0081] 1. Collection of samples and collation of sample data

[0082] The inventor of the present application collected plasma samples that meet the standards with standard operating procedures, systematically collected complete demographic data, clinical data, etc., and through sorting out the sample data, the inventor selected plasma samples from 707 people as miRNA chip detection and Subsequent series of experimental samples verified by qRT-PCR, these 707 people include 112 patients with cervical cancer, 101 patients with cervical precancerous lesions, 185 patients with benign cervical diseases, 242 healthy controls and 67 patients with benign and malignant diseases of other tissues and organs .

[0083] The inclusion criteria for the cervical cancer and its precancerous lesions group were: first-diagnosed, untreated patients with cervical cancer and its precancer...

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Abstract

The invention discloses a kit for detecting cervical cancer and precancerous lesions thereof and an application thereof. The present invention provides an application of a substance for detecting thefollowing RNAs substances in preparation of a product for the diagnosis or auxiliary diagnosis of cervical cancer and / or precancerous lesions thereof: hsa-MiR-20a, hsa-MiR-625-3p, hsa-MiR-1274a, hsa-MiR-26b, hsa-MiR-484, hsa-MiR-574-3p, hsa-MiR-191, hsa-MiR-1274b and hsa-MiR-146b. As the miRNA combination of the invention is used for screening cervical cancer and precancerous lesions thereof, therisk of cervical cancer and / or precancerous lesions thereof can be early war, and the early diagnosis ratio can be improved. Plasma miRNA detection kit based on this method needs only patient plasma and no other tissues. The sensitivity of detection is improved by quantitative PCR detection of plasma miRNA level, and the detection methods of cervical cancer and its precancerous lesions are enriched to increase the proportion of early detection of cervical cancer and its precancerous lesions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for cervical cancer and its precancerous lesion and its application. Background technique [0002] Cervical cancer is one of the most common malignant tumors in women, accounting for the second place among female malignant tumors nationwide, second only to breast cancer. According to statistics, there are about 500,000 new cases of cervical cancer every year in the world, accounting for 5% of all new cancer cases, and more than 80% of the cases occur in developing countries. There are approximately 130,000 new cases of cervical cancer in my country every year, accounting for 28% of the total number of new cases of cervical cancer in the world. According to the "2017 China Cancer Registration Annual Report", the incidence rate of cervical cancer in my country is 15.17 / 100,000, and the mortality rate is 3.98 / 100,000. Cervical cancer has become an important disease tha...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 杨春花郭弘妍张智刘莹莹邢婉丽程京
Owner CAPITALBIO CORP
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