Method for extracting and purifying hyaluronic acid in cockscomb

A technology of hyaluronic acid and purification method, which is applied in the field of extraction and purification of hyaluronic acid in cockscombs, can solve the problems of low yield, excessive absorption, incomplete absorption, etc., and achieve the effect of shortening the extraction process

Inactive Publication Date: 2019-01-18
安徽万士生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hyaluronic acid is an acidic mucopolysaccharide widely used in ophthalmic surgery and cosmetics. Hyaluronic acid can be obtained from cockscombs by distillation extraction, chloroform deproteinization, ethanol precipitation and purification. , ethanol precipitation and purification can be obtained from cockscomb hyaluronic acid. When the water phase is absorbed, it is carried out by siphon. When the upper water phase is absorbed, it is easy to over-absorb or incompletely absorb. In turn, the purity of hyaluronic acid is low or the yield is low

Method used

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  • Method for extracting and purifying hyaluronic acid in cockscomb
  • Method for extracting and purifying hyaluronic acid in cockscomb
  • Method for extracting and purifying hyaluronic acid in cockscomb

Examples

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Effect test

Embodiment 1

[0036] Wash the fresh cockscombs in a 70°C water bath to remove blood clots, take 10kg of pretreated cockscombs, divide them into ten parts, immerse them in acetone for 3-5 hours until the cockscombs harden, then take out the hardened cockscombs and dry them, crush the hardened cockscombs, and add 6-7 times Deionized water, let stand and take out the filtrate, then collect the residue and add deionized water again, stand still to get the extract, and repeat three to four times, add the above extract to ethanol and chloroform and n-butyl Mixed liquid of alcohol solution, stirring and standing still, wherein chloroform and n-butanol solution are proportioned according to 1:3.8, the upper water phase is taken out by siphon method, phase-liquid separation is carried out, the upper water phase is obtained, and the upper water phase is Add 2 to 3 times the volume of ethanol, after standing still, add ethanol and the mixed liquid of chloroform and n-butanol solution equal to the volum...

Embodiment 2

[0038] Wash the fresh chicken combs in a 70°C water bath to remove blood clots. Take 10kg of pretreated chicken combs, divide them into ten parts, soak them in acetone for 4.5 hours until the combs harden, then take out the hardened combs and dry them, crush the hardened combs, and add 6-7 times deionized water, let it stand still and take out the filtrate, then collect the residue and add deionized water again, stand still to get the extract, and repeat three to four times, add the above extract liquid to ethanol and chloroform and n-butanol solution equal to the volume of the extract and ethanol The mixed liquid is stirred and left still, wherein the chloroform and n-butanol solution are proportioned according to 1:4.3, the upper water phase is taken out by siphon method, phase-liquid separation is carried out, the upper water phase is obtained, and the upper water phase is added 2 to 3 times the volume of ethanol, after standing still, add ethanol and the mixed liquid of chl...

Embodiment 3

[0040] Wash the fresh chicken combs in a 70°C water bath to remove blood clots. Take 10kg of pretreated chicken combs, divide them into ten parts, soak them in acetone for 4.5 hours until the combs harden, then take out the hardened combs and dry them, crush the hardened combs, and add 6-7 times deionized water, let it stand still and take out the filtrate, then collect the residue and add deionized water again, stand still to get the extract, and repeat three to four times, add the above extract liquid to ethanol and chloroform and n-butanol solution equal to the volume of the extract and ethanol The mixed liquid is stirred and left still, wherein the chloroform and n-butanol solution are proportioned according to 1:4.3, and the water phase is separated through the liquid overflow barrel 1, wherein the liquid overflow barrel 1 includes an inner barrel 5 and an outer barrel 6, and the inner barrel 5 is installed There is a ball screw 7, a number of "eight"-shaped connectors 3 a...

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Abstract

The invention discloses a method for extracting and purifying hyaluronic acid in cockscomb and relates to the technical field of hyaluronic acid extraction. The method comprises the following steps: adopting acetone solution for hardening cockscomb and smashing; adopting a mixed solution of ethyl alcohol, chloroform and n-butyl alcohol solution for extracting hyaluronic acid; repeatedly filteringand cleaning coarse substances; adopting acetic acid for acidizing, alkalizing and adding pronase for decomposing nucleic acid in cells, thereby further promoting the purity of hyaluronic acid. According to the invention, a fluid diffusing barrel is adopted for performing water phase separation on the solution, and the operation is simple, convenient and quick, so that the process flow for extracting hyaluronic acid in cockscomb is shortened.

Description

technical field [0001] The invention belongs to the technical field of hyaluronic acid extraction, in particular to a method for extracting and purifying hyaluronic acid from cockscombs. Background technique [0002] Hyaluronic acid is an acidic mucopolysaccharide widely used in ophthalmic surgery and cosmetics. Hyaluronic acid can be obtained from cockscombs by distillation extraction, chloroform deproteinization, ethanol precipitation and purification. , ethanol precipitation and purification can be obtained from cockscomb hyaluronic acid. When the water phase is absorbed, it is carried out by siphon. When the upper water phase is absorbed, it is easy to over-absorb or incompletely absorb. And then make hyaluronic acid purity low or low output. Contents of the invention [0003] The purpose of the present invention is to provide a method for extracting and purifying hyaluronic acid in cockscombs, which is simple, convenient and quick to separate the liquid through a liq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0072
Inventor 徐双贵胡培
Owner 安徽万士生物制药有限公司
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