Application of Cordyceps sinensis exopolysaccharide in probiotic health food and/or probiotic traditional Chinese medicine

A technology of Cordyceps sinensis cells and health food, applied in the field of polysaccharides, can solve the problems of limited application of Cordyceps sinensis exopolysaccharides, and achieve the effects of protecting viability, increasing use value, and promoting growth

Active Publication Date: 2022-04-29
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, and provide a kind of Cordyceps sinensis exopolysaccharide in probiotic health food and / or probiotic traditional Chinese medicine as the application of promoting the growth of probiotics and / or protecting the viability of probiotics, Aiming at solving the technical problem of limited application of exopolysaccharide of Cordyceps sinensis

Method used

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  • Application of Cordyceps sinensis exopolysaccharide in probiotic health food and/or probiotic traditional Chinese medicine
  • Application of Cordyceps sinensis exopolysaccharide in probiotic health food and/or probiotic traditional Chinese medicine
  • Application of Cordyceps sinensis exopolysaccharide in probiotic health food and/or probiotic traditional Chinese medicine

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Experimental program
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Embodiment 1

[0028] Fresh wild Cordyceps sinensis was collected in the Cordyceps producing area (Sichuan-Tibet Plateau, China) in early June, and the fungus was isolated and purified from the fruiting bodies after full washing, surface disinfection and sterilization in the laboratory. The fungi were identified as Cordyceps-related fungi by morphology and gene identification. Strain isolation and mycelium preservation adopt PDA solid medium at a temperature of 20°C (long-term storage temperature of 4°C).

[0029] The Cordyceps fungus preserved in solid PDA medium obtained from wild Cordyceps sinensis is inoculated into liquid medium for mycelium fermentation culture. The liquid medium composition: glucose 40g / L, peptone 5g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.5g / L, yeast extract 10g / L; 50mL per bottle, autoclave (121°C, 20min), after cooling to room temperature, insert the Cordyceps sinensis mycelia for solid culture, place the culture solution inserted i...

Embodiment 2

[0031] Add EPS-US instead of glucose (Glc) or galactooligosaccharide (GOS) as a carbon source to the enhanced Clostridium-RCM liquid medium at a concentration of 5g / L, autoclave (121°C, 20min), and press volume Fraction 1% was inserted into the fermentation liquid of Bifidobacterium B.adolescentis and Bifidobacterium B.bifidum cultured in RCM for 48 hours, cultured at 37°C, anaerobic environment, and 200rpm for 48 hours, and then the fermentation liquid was tested and analyzed. Compared with the control group (control) without carbon source, the bacterial concentration OD value (600nm) of the EPS-US group increased as follows: figure 1 and figure 2 shown. figure 1 and figure 2 Among them, Control group: control without any carbon source; Glc group: glucose Glc as carbon source; GOS group: galactooligosaccharide GOS as carbon source; EPS-US group: EPS-US as carbon source; Compared with the control group, * indicates significant difference, Pfigure 1 and figure 2 It can b...

Embodiment 3

[0037] Add EPS-US instead of glucose as a carbon source to the enhanced Clostridium-RCM liquid medium at a concentration of 5g / L, autoclave (121°C, 20min), and then insert 1% by volume into RCM cultured for 48 hours Bifidobacteria B.adolescentis, B.bifidum, B.adolescentis, B.bifidum, B.breve, B.infantis and B.longum fermentation liquid, cultivated at 37°C, anaerobic environment, 200rpm for 20 hours, 48 ​​hours or 6 days, then the fermentation broth was taken out and diluted into different concentrations (10 -1 ,10 -2 ,10 -3 …10 -10 ), inoculated on the RCM solid medium, cultivated for 2 days, and the results are shown in Table 2. Table 2 is the impact of Cordyceps sinensis exopolysaccharide and other carbon sources on the colony forming unit (CFU) of bifidobacteria solid culture (×10 8 / mL, n=3), wherein, Control group: control group without any carbon source; Glc group: glucose as carbon source; GOS group: galactooligosaccharide as carbon source; EPS-US group: Ultrasonic...

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Abstract

The invention belongs to the technical field of polysaccharides, and in particular relates to the application of Cordyceps sinensis exopolysaccharide in probiotic health food and / or probiotic traditional Chinese medicine as an auxiliary material for promoting the growth of probiotics and / or protecting the viability of probiotics. The present invention is verified by experiments that the exopolysaccharide of Cordyceps sinensis can promote the growth of probiotics, and can protect the viability of probiotics. Therefore, it has a wide range of applications in the production fields of probiotic traditional Chinese medicine, probiotic health food, etc., and can significantly improve the production of probiotics. use value.

Description

technical field [0001] The invention belongs to the technical field of polysaccharides, and in particular relates to the application of Cordyceps sinensis exopolysaccharides in probiotic health food and / or traditional Chinese medicine. Background technique [0002] Cordyceps sinensis (Berk.) Sace is a medicinal fungus of the genus Cordyceps in the family Clavicipiraceae. Cordyceps is rich in physiologically active substances, has unique medicinal value and a wide range of pharmacological effects, and polysaccharide is one of the main active ingredients. [0003] Due to the strict parasitic and special ecological environment requirements of natural Cordyceps sinensis, its resources are extremely limited and expensive. In recent years, with the success of the research on the anamorph of Cordyceps sinensis and the continuous development of artificial fermentation culture, the research on polysaccharides from Cordyceps sinensis has gradually increased, but there is no Literatur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/125A23L33/135A61K31/715A61P1/00
CPCA23L33/125A23L33/135A61K31/715A23V2002/00A23V2250/51
Inventor 吴建勇宋昂芯
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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