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Reagent for gene expression analysis of eggplant and internal reference gene thereof

A technology of gene expression, eggplant, applied in the field of biology

Active Publication Date: 2019-01-25
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on reference genes applicable to different fruit development stages of eggplant under low temperature conditions

Method used

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  • Reagent for gene expression analysis of eggplant and internal reference gene thereof
  • Reagent for gene expression analysis of eggplant and internal reference gene thereof
  • Reagent for gene expression analysis of eggplant and internal reference gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Establishment of internal reference genes in gene expression analysis of different fruit development stages of eggplant under low temperature conditions and normal temperature conditions

[0035] 1 test material

[0036] Eggplant parthenocarpic line D-10 and eggplant non-parthenocarpic line 03-2 (Li Yanwei, Liu Fuzhong, Chen Yuhui, Zhang Ying, Zhang Weiwei, Lian Yong. Effect of temperature on endogenous hormones during eggplant parthenocarpic ovary (fruit) development The impact of content. Chinese Vegetables 2013 (22): 32-38) is a strain bred by the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. The public can obtain the biological material from the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. It is used for related experiments of the invention and cannot be used for other purposes.

[0037] D-10 is a low temperature tolerant inbred line of eggplant, the fruit can expand n...

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Abstract

The invention discloses a reagent for eggplant gene expression analysis and an internal reference gene used for the reagent. The invention provides any one of M1-M3 as an internal reference gene in eggplant gene expression analysis: M1, a set of genes consisting of D1 and D4 genes; M2, D1; M3, D4; in the M1-M3, the D4 is a gene encoding a protein whose amino acid sequence is SEQ ID No.4; D1 is a gene encoding a protein having an amino acid sequence of SEQ ID NO:6. The invention not only provides reliable internal reference genes for standardized quantification of gene expression levels at different fruit development stages of eggplant under low temperature and / or normal temperature conditions. The invention will further promote the research of low-temperature resistance genes in differentfruit development stages of eggplant.

Description

technical field [0001] The invention relates to a reagent for gene expression analysis of eggplant and an internal reference gene used in the field of biology. Background technique [0002] Eggplant (Solanum melongena L.) is one of the most popular vegetable crops in the world. Like most temperature-loving vegetables, eggplant is not easy to set fruit under low temperature conditions, which seriously affects the yield and quality of eggplant (NothmannJ, Koller D.Effects of growth regulators on fruit and seed development ineggplant(Solanum melongena L.).J Hort Sci. 1975;50:23-7). In order to avoid the heavy loss caused by low temperature, the method of cultivating low temperature resistant eggplant varieties is often adopted in production. However, the main genes related to low temperature resistance in eggplant have not yet been excavated, and the molecular mechanism of low temperature resistance in eggplant needs further study. [0003] When conducting functional gene sc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/158C12Q2600/166
Inventor 刘富中张映杨锦坤陈钰辉连勇
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI