A kind of biphasic medium for urogenital mycoplasma

A biphasic culture medium and genitourinary tract technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of time-consuming and labor-intensive detection process, false positive of liquid culture method, low accuracy, etc., to achieve Time-saving, accurate solid culture, and high specificity

Active Publication Date: 2019-10-08
山东恒辰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the shortcomings of the current urogenital tract detection, the liquid culture method has false positives, and the solid medium detection is too time-consuming, and requires professional equipment carbon dioxide incubator and microscope observation. The entire detection process is time-consuming and laborious and the accuracy is not good. high problem

Method used

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  • A kind of biphasic medium for urogenital mycoplasma
  • A kind of biphasic medium for urogenital mycoplasma
  • A kind of biphasic medium for urogenital mycoplasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of urogenital mycoplasma biphasic culture medium, comprises two parts of liquid culture medium and solid culture medium, and solid culture medium is placed in container, forms a slope, and 1 / 4 of described slope is submerged in liquid culture medium simultaneously; Said container also includes a sealing part that can seal the internal space of said container; wherein the composition of liquid culture medium comprises: 2g / L yeast extract powder, 2g / L beef heart extract powder, 2g / L soybean peptone, 1g / L chlorine Sodium chloride, 1g / L dipotassium hydrogen phosphate, 0.05g / L manganese sulfate, 0.01g / L cysteine ​​hydrochloride, 0.5g / L urea, 0.5g / L arginine, 0.1g / L glutamine Amide, 300mL / L horse serum, 5mL / L DMEM liquid medium, 0.05g / L sodium pyruvate, 1mL / L mass volume percent 0.1% phenol red indicator, 0.001g / L nystatin and 300IU / L penicillin ; The composition of the solid medium is the same as that of the liquid medium except for the 10g / L agar.

[0033] There is ...

Embodiment 2

[0047] A two-phase culture medium for mycoplasma urogenital tract, comprising two parts of a liquid culture medium and a solid culture medium, the solid culture medium is placed in a container to form a slope, while 1 / 3 of the slope is submerged in the liquid culture medium; Said container also includes a sealing part that can seal the internal space of said container; wherein the composition of liquid culture medium comprises: 4.5g / L yeast extract powder, 4.5g / L beef heart extract powder, 4.5g / L soybean peptone, 4g / L Sodium Chloride, 4g / L Dipotassium Hydrogen Phosphate, 0.4g / L Manganese Sulfate, 2g / L Cysteine ​​Hydrochloride, 3g / L Urea, 3g / L Arginine, 1.5g / L Glutamine Amide, 400mL / L horse serum, 22mL / L LDMEM liquid medium, 5g / L sodium pyruvate, 4mL / L mass volume percent 0.1-0.5% phenol red indicator, 0.01g / L nystatin and 3000IU / L penicillin ; The composition of the solid medium is the same as that of the liquid medium except for 10-30 g / L agar.

[0048] There is also a filt...

Embodiment 3

[0062] A two-phase culture medium for mycoplasma urogenital tract, including liquid culture medium and solid culture medium, the solid culture medium is placed in a container to form a slope, while 1 / 2 of the slope is submerged in the liquid culture medium; Said container also includes a sealing part that can seal the internal space of said container; wherein the composition of liquid culture medium comprises: 10g / L yeast extract powder, 10g / L beef heart extract powder, 10g / L soybean peptone, 10g / L chlorine Sodium chloride, 10g / L dipotassium hydrogen phosphate, 1.5g / L manganese sulfate, 8g / L cysteine ​​hydrochloride, 8g / L urea, 8g / L arginine, 4.0g / L glutamine, 500mL / L horse serum, 50mL / LDMEM liquid medium, 15g / L sodium pyruvate, 10mL / L mass volume percentage is 0.5% phenol red indicator, 0.050g / L nystatin and 8000IU / L penicillin; Except for the 30g / L agar, the other components are the same as those of the liquid medium.

[0063] There is also a filter screen between the seal...

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Abstract

The invention discloses a biphasic media for genitourinary tract mycoplasma, and belongs to the field of microbiological detection. The biphasic media comprises a liquid medium and a solid medium, wherein the solid medium is arranged in a container and forms an inclined plane; meanwhile 1 / 4 to 1 / 2 of the inclined plane is immersed into the liquid medium; the biphasic media mainly comprises the following components: yeast extract powder, beef heart infusion, soy peptone, sodium chloride, dipotassium phosphate, manganese sulfate, cysteine hydrochloride, urea, arginine, glutamine, equinum serum,a DMEM liquid medium, sodium pyruvate, a phenol red indicator, nysfungin, penicillin and agar; the container further comprises a sealing component capable of sealing the inner space of the container and a filter screen; a carbon dioxide producing piece is arranged in the filter screen, which can absorb moisture during culturing and release carbon dioxide, so that carbon dioxide incubators are notrequired any more. The whole process is simple to operate, the specificity is high, and laboratory operation is easy.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and in particular relates to a urogenital mycoplasma culture medium. Background technique [0002] Mycoplasma, also known as mycoplasma, is one of the simplest prokaryotes found so far, which is between viruses and bacteria, has no cell wall, is highly pleomorphic, and can pass through bacteria filters. At least 16 species of mycoplasma have been found to inhabit the human body, 7 of which are opportunistic pathogenic bacteria, and among these 7 species, mycoplasma hominis (Mh) and ureaplasma urealyticum (Uu) are the most common, and can be found in about 70% detected in the genitourinary system of sexually active individuals. Urogenital Mycoplasma is the second most common pathogen causing nongonococcal urethritis and cervicitis. About 20-30% of nongonococcal urethritis is caused by Mh and Uu infection. The bacterial infection can even cause serious consequences such as cervicitis, postp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/35
Inventor 廖爱美范立飞张金蓬
Owner 山东恒辰生物科技有限公司
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