Medium for co-cultivation of yeast and lactic acid bacteria and preparation method thereof

A culture medium and lactic acid bacteria technology, applied in the field of microorganisms, can solve problems such as complex production operations, and achieve long storage time, simple ingredients, and good stability

Active Publication Date: 2019-10-25
广西满意宝农牧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some vegetables such as carrots, cucumbers, tomatoes, etc. can also be used as a medium for the mixed culture of yeast and lactic acid bacteria, but a large amount of carbon and nitrogen sources need to be added, which makes the production operation too complicated

Method used

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  • Medium for co-cultivation of yeast and lactic acid bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A culture medium for co-cultivation of yeast and lactic acid bacteria, comprising the following components in parts by weight:

[0033] 5 parts of brown rice, 6 parts of brown sugar and 122 parts of purified water.

[0034] The medium provided by this program contains brown rice and brown sugar, and brown rice is rich in starch, protein, fat, vitamins and 11 kinds of minerals. Brown sugar contains glucose, fructose and other monosaccharide and polysaccharide energy substances, folic acid, trace substances, amino acids, cellulose and other substances, and organic acids such as cellulose and polysaccharides, which provide rich carbon sources for yeast and lactic acid bacteria. Nitrogen source and other growth factors meet the growth and reproduction needs of yeast and lactic acid bacteria.

[0035] A preparation method for a medium for co-cultivation of yeast and lactic acid bacteria, comprising the following steps:

[0036] Step 1, wash 5 parts by weight of brown rice,...

Embodiment 2

[0040] On the basis of embodiment 1, after said step three, also include:

[0041] Step 4, add wood vinegar to the medium prepared in step 3 to contain 0.15mL wood vinegar per kilogram of medium, mix well, then add buffer solution to adjust the pH value of the medium to 5 in the medium . Provide a suitable acidity environment for the growth of yeast and lactic acid bacteria, and add wood vinegar to the medium to promote the growth of mycelium and fruiting bodies.

[0042] In the preparation method of the medium for the co-cultivation of yeast and lactic acid bacteria, the buffer solution is a HAc-NaAc buffer solution with a pH value of 7.

Embodiment 3

[0044]On the basis of Example 1, after the brown rice is washed in the step 1, before cooking, it also includes:

[0045] After the grapes are crushed, mixed with brown rice and soybean powder in a mass ratio of 0.5:1:0.5, immersed in 15 parts by weight of water, then 0.4 parts by weight of cellulase, 0.5 parts by weight of pectinase, 0.12 parts by weight of pectinase are added to the water. Amylase in parts by weight and protease in 0.5 parts by weight, and the pH value is adjusted to 6, enzymolysis is carried out at a temperature of 50° C. for 2.5 hours, and then cooked for 25 minutes to obtain brown rice. High-temperature cooking can deactivate the enzyme, so that the enzyme does not Hydrolyzes the cell wall of yeast. Enzymolysis of grapes, brown rice and soybean flour, which can destroy the cell walls of brown rice and grapes, is conducive to the hydrolysis of cellulose, pectin, starch and protein into glucose and amino acids, which can be directly used by yeast and lactic...

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PUM

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Abstract

The invention discloses a culture medium for saccharomycetes and lactic acid bacteria co-culture and a preparation method of the culture medium. The culture medium comprises the following constituents of, by weight, 4 to 6 parts of brown rice, 5 to 7 parts of brown sugar and 112 to 132 parts of purified water. The preparation method includes the following steps that firstly, the 4 to 6 parts by weight of brown rice is cooked into steamed brown rice; secondly, after 2 parts by weight of purified water is added to the 5 to 7 parts by weight of brown sugar, the brown sugar is dissolved, sterilization is conducted, and brown sugar water is prepared; and thirdly, the steamed brown rice, the brown sugar water and the purified water are mixed, and the culture medium is prepared. The prepared culture medium has the simple constituents, can meet the requirements for saccharomycetes and lactic acid bacteria breeding and is good in stability, low in cost and long in shelf time.

Description

technical field [0001] The present invention relates to the field of microorganisms. More specifically, the present invention relates to a medium for co-cultivation of yeast and lactic acid bacteria and a preparation method thereof. Background technique [0002] Lactic acid bacteria is a general term for a group of bacteria that can ferment carbohydrates and mainly produce large amounts of lactic acid. They are usually Gram-positive bacteria and non-pathogenic bacteria. In the process of growth and metabolism, lactic acid bacteria not only produce enzymes for their own growth, but also produce enzymes with special functions, such as bifidofactors, which can not only promote the growth of bifidobacteria, but also increase the value of beneficial bacteria in the intestinal tract special physiological functions. The metabolism of lactic acid bacteria can also produce organic acids, which can reduce the pH value in the gastrointestinal tract. The acidic environment can prevent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/20C12R1/25
CPCC12N1/16C12N1/20
Inventor 韦爱芬苏善高陈文艳
Owner 广西满意宝农牧科技有限公司
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