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Kit for detecting expression level of breast cancer related genes

A technology for gene expression levels and detection reagents, applied in the field of kits for detection of breast cancer-related gene expression levels, can solve the problems of complex operations, long time-consuming, high detection costs, etc., and achieve strong specificity, high sensitivity, and short time-consuming effects

Inactive Publication Date: 2019-03-08
GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are either time-consuming, complicated to operate, or have high requirements for operators and experimental sites, or have high detection costs, or are difficult to detect different mutation sites of multiple genes at the same time

Method used

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  • Kit for detecting expression level of breast cancer related genes
  • Kit for detecting expression level of breast cancer related genes
  • Kit for detecting expression level of breast cancer related genes

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Embodiment Construction

[0038] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0039] 1. Detection of mutation sites

[0040] 1.1 Detection technology and instruments

[0041] In order to quickly and high-throughput detect these mutations, we use microfluidic technology, including two parts: microfluidic chip and microfluidic platform.

[0042] The microfluidic chip integrates microfluidic channels, valves and reaction chambers on one chip, and automatically completes the reaction system mixing through the air pressure, temperature control system and fluorescence analysis system to achieve up to 9216 PCR reactions. The steps and time of manual sample addition are greatly reduced, and the whole process takes about 2 hours to complete.

[0043] Many micro-channels (sample, reagent, control liq...

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PUM

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Abstract

The invention relates to a kit for detecting the expression level of breast cancer related genes. The kit contains a detection reagent for detecting the expression level of one or more of BAG1, BCL2 CCNB1, CD68, SCUBE2, CTSL2, ESR1, GRB7, GSTM1, ERBB2, MKI67, MYBL2, PRG, AURKA, MMP11 and BIRC5 genes. With the adoption of the kit, the expression level of the breast cancer related genes can be subjected to high-throughput detection simultaneously under the same reaction condition. The detection is high in specificity and high in sensitivity and consumes short time, the lowest detection limit is1 ng / mu L, and the process from specimen inspection to result obtaining can be completed within 2 h.

Description

technical field [0001] The invention relates to the field of detection of breast cancer genes, and more particularly relates to a kit for detecting the expression level of breast cancer-related genes. Background technique [0002] Breast cancer is currently the cancer with the highest incidence rate in women. In 2012, there were about 1.7 million patients worldwide, of which 521,900 patients died of breast cancer, ranking first in the incidence of female cancer in my country. [0003] For patients with early breast cancer with estrogen receptor (ER) positive, it is an urgent problem to be solved whether to use endocrine therapy alone or add chemotherapy so that patients can avoid over-treatment and avoid metastasis and recurrence. However, there are many genes related to breast cancer, and the cost of each detection is huge, and doctors will be submerged in massive data and ignore effective data. Therefore, it is necessary to select genes that can be used as indicators for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 何益龙萧锦华王明璐甘海燕杨呈勇
Owner GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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