Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of small molecule astragalus chelating peptide

A technology for chelating peptides and small molecules, applied in the field of biological peptide separation, can solve the problems of incomplete extraction of heat-sensitive components, easy damage, and insufficient effect of astragalus, so as to improve chelation efficiency, improve stability, and make up for peptide content. low effect

Active Publication Date: 2021-08-03
河北肽都生物科技集团有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that in the existing astragalus extraction process, the active ingredients are not fully extracted and the heat-sensitive components are easily destroyed, resulting in the inability to fully exert the efficacy of astragalus, the present invention provides a method for preparing a small-molecule astragalus chelating peptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of small molecule astragalus chelating peptide:

[0040] Step 1. Select 100 g of dried Astragalus membranaceus, crush it, add 700 ml of purified water, stir evenly, and decoct at 90°C for 5 hours to obtain a decoction mixture;

[0041] Step 2: Cool the decoction mixture to 55°C, add 1g of pectinase and 0.5g of α-L-rhamnosidase, and stir at a speed of 75r / min for 20 minutes to obtain the first enzymolysis solution ;

[0042] Step 3: Add 1 g of Luo Han Guo protease, 0.5 g of phytase and 0.4 g of laccase to the first enzymolysis solution, stir evenly, heat up to 55° C., and stir the enzymolysis at a speed of 75 r / min for 4 hours to obtain the second enzymatic solution;

[0043] Step 4: Warm up the second enzymolysis solution to 90°C, and inactivate the enzyme for 15 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifu...

Embodiment 2

[0048] A preparation method of small molecule astragalus chelating peptide:

[0049] Step 1. Select 10Kg of dried Astragalus membranaceus, crush it, add 75L of purified water, stir evenly, and decoct at 92°C for 4.5 hours to obtain a decoction mixture;

[0050] Step 2, cooling the decoction mixture to 56°C, adding 120g of pectinase and 60g of α-L-rhamnosidase, and stirring at a speed of 75r / min for 25min to obtain the first enzymolysis solution;

[0051] Step 3: Add 120g Luo Han Guo protease, 60g phytase and 50g laccase to the first enzymolysis solution, stir evenly, raise the temperature to 56°C, and stir the enzymolysis at a speed of 75r / min for 3.5h to obtain the second enzyme Solution;

[0052] Step 4: Warm up the second enzymolysis solution to 92°C, and inactivate the enzyme for 12 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifuge fi...

Embodiment 3

[0057] A preparation method of small molecule astragalus chelating peptide:

[0058] Step 1. Select 100Kg of dried Astragalus membranaceus, crush it, add 800L of purified water, stir evenly, and decoct at 95°C for 4 hours to obtain a decoction mixture;

[0059] Step 2: Cool down the decoction mixture to 58°C, add 1.5Kg of pectinase and 800g of α-L-rhamnosidase, and stir the enzymolysis at 75r / min for 20min to obtain the first enzymolysis solution ;

[0060] Step 3: Add 1.5Kg Luo Han Guo protease, 800g phytase and 600g laccase to the first enzymolysis solution, stir evenly, heat up to 58°C, and stir the enzymolysis at a speed of 75r / min for 3 hours to obtain the second enzyme Solution;

[0061] Step 4: Warm up the second enzymolysis solution to 95°C, and inactivate the enzyme for 10 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the rotational speed of the decanter centrifuge is 3600r / min; Centrifuge filtra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method of a small molecule astragalus chelating peptide, comprising the following steps: pulverizing the astragalus root, decocting it with water; then adding pectinase and α-L-rhamnosidase, stirring for enzymolysis; and then adding Luo Han Guo protease , phytase and laccase, and continue enzymolysis; inactivate the enzyme in the enzymatic hydrolyzate, separate and purify, add soybean peptide, heat up to 45-50°C, and stir for 30-45min to obtain a chelating solution. Concentrate and dry to obtain small molecule astragalus chelating peptide. The astragalus chelating peptide product prepared by the invention has high purity, and the peptide content with a peptide molecular weight of 180-500 daltons can reach more than 99%. Through the enzymolysis of the compound enzyme, not only the allergens in the astragalus are degraded, but also the degraded It eliminates substances in Astragalus that are difficult for the human body to digest and utilize, and effectively avoids the problem of aggravating the gastrointestinal burden caused by long-term use of Astragalus. It can be widely used in food, health care products, medicines and other fields.

Description

technical field [0001] The invention relates to the technical field of separation of biological peptides, in particular to a preparation method of a small molecule astragalus chelating peptide. Background technique [0002] A variety of amino acids and minerals are essential nutrients for the human body, and these nutrients are mainly obtained from food. After food enters the human body and is digested by the stomach and small intestine, the protein is hydrolyzed into amino acids and some small molecule short peptides. The absorption of small peptides and free amino acids are two independent transport systems. It has the characteristics of low energy, not easy to saturate, and the transport between various peptides is non-competitive and inhibitory. The latest research shows that small peptides can improve the absorption and utilization of mineral elements, that is, small peptides can form chelates with Ca, Zn, Cu, Fe and other mineral elements to increase their solubility ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com