Thyroid Cancer Molecular Diagnostic Kit
A technology for thyroid cancer and molecular diagnosis, applied in biochemical equipment and methods, microbial determination/examination, etc., can solve problems such as complex detection, achieve high repeatability, simple and convenient operation, and avoid physical and psychological discomfort Effect
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[0069] By capturing and collecting circulating tumor cells in the peripheral blood of patients, and detecting the expression of special genes in circulating tumor cells, it is possible to identify benign and malignant thyroid nodules in patients. A total of 109 subjects were enrolled, including 20 healthy subjects, 36 patients with benign thyroid nodules, and 53 patients with thyroid cancer. The following steps were used to enrich and capture circulating epithelial cells in peripheral blood for qPCR detection.
[0070] 1. Isolation of monocytes
[0071] 1) Collect 8 mL of peripheral blood from healthy people and patients with EDTA tubes.
[0072] 2) Dilute 8 mL of blood 1-fold with PBS.
[0073] 3) Add 15mL of Ficoll lymphocyte separation medium to a 50mL centrifuge tube, and carefully add the diluted blood to the upper layer of the separation medium.
[0074] 4) Centrifuge at 400g room temperature for 30min.
[0075] 5) If stratification occurs after centrifugation, take ...
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