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A technology for thyroid cancer and molecular diagnosis, applied in biochemical equipment and methods, microbial determination/examination, etc., can solve problems such as complex detection, achieve high repeatability, simple and convenient operation, and avoid physical and psychological discomfort Effect
Active Publication Date: 2022-04-12
BEIJING USCI MEDICAL LAB CO LTD
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[0006] The main purpose of the present invention is to provide a molecular diagnostic kit for thyroid cancer to solve the problem of complex detection in the prior art
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Embodiment 1
[0069] By capturing and collecting circulating tumor cells in the peripheral blood of patients, and detecting the expression of special genes in circulating tumor cells, it is possible to identify benign and malignant thyroid nodules in patients. A total of 109 subjects were enrolled, including 20 healthy subjects, 36 patients with benign thyroid nodules, and 53 patients with thyroid cancer. The following steps were used to enrich and capture circulating epithelial cells in peripheral blood for qPCR detection.
[0070] 1. Isolation of monocytes
[0071] 1) Collect 8 mL of peripheral blood from healthy people and patients with EDTA tubes.
[0072] 2) Dilute 8 mL of blood 1-fold with PBS.
[0073] 3) Add 15mL of Ficoll lymphocyte separation medium to a 50mL centrifuge tube, and carefully add the diluted blood to the upper layer of the separation medium.
[0074] 4) Centrifuge at 400g room temperature for 30min.
[0075] 5) If stratification occurs after centrifugation, take ...
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Abstract
The invention provides a thyroid cancer molecular diagnosis kit. The kit includes detection reagents for at least three of the following genes: CK19, survivin, TG, LGALS3, MET, TFF3, SERPINA1, TIMP1, FN1, TPO, TGFA, QPCT, CRABP1, FCGBP, EPS8, PROS1, LRP4, DPP4, GJB3 , ST14, EVA1, SPUVE, HBB, MKRN2, MRC2, IGSF1, KIAA0830, RXRG, P4HA2, CDH3, IL13RA1, MTMR4, MDK, CITED1, CHI3L1, ODZ1, N33, SFTPB, and SCEL. The benign and malignant thyroid nodules are judged by the level of expression of thyroid cancer tissue-specifically expressed genes, and the operation is simple and convenient.
Description
technical field [0001] The invention relates to the field of auxiliary diagnostic kits, in particular to a molecular diagnostic kit for thyroid cancer. Background technique [0002] Thyroid cancer is a common malignancy of the head and neck, and its pathological types include papillary thyroid carcinoma, follicular thyroid carcinoma, anaplastic thyroid carcinoma, and medullary thyroid carcinoma. Among them, papillary thyroid carcinoma and follicular thyroid carcinoma are called differentiated thyroid cancer because of the better differentiation of cancer cells, accounting for more than 90% of thyroid cancer. According to relevant literature reports, the incidence of thyroid cancer is increasing year by year at a rate of 4% in the world, becoming the fastest growing cancer. According to the latest data released by the National Cancer Center in 2017, the overall incidence rate of thyroid cancer is 10.16 / 100,000, ranking the seventh in the incidence of malignant tumors, and th...
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