Storage liquid of falling cells in human excrement sample
A technology for feces samples and exfoliated cells, which is applied in the preservation of human or animal bodies, the measurement/inspection of microorganisms, biochemical equipment and methods, etc. It can solve the problems of cell lysis and DNA extraction failure, and achieve simple proportioning and low cost. Low cost, effect of improving the quality of cell preservation
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[0030] Example 1
[0031] The preservation solution for storing cells in human feces is characterized in that it contains 29.2g EDTA dry powder, 60.55g Tris dry powder, 0.58g NaCl dry powder in 1 L of preservation solution, and the balance is sterilized water.
[0032] The method for preparing a preservation solution for exfoliated cells in human feces includes the following steps:
[0033] 1) Pour 29.2g of EDTA dry powder into a beaker, dissolve it with a small amount of sterile water, add sterile water to 200ml after the dissolution is complete to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.
[0034] 2) Pour 60.55g of Tris dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterile water to 500ml to obtain a 1mol / L Tris solution.
[0035] 3) Pour the 2.92Nacl dry powder into a beaker, dissolve it with a small amount of sterile water, and add sterile water to 10ml a...
Example Embodiment
[0038] Example 2
[0039] The preservation solution for preserving cells in human feces is characterized in that it contains 25g EDTA dry powder, 65g Tris dry powder, 0.2g NaCl dry powder in 1L of preservation solution, and the balance is sterilized water.
[0040] The method for preparing a preservation solution for exfoliated cells in human feces includes the following steps:
[0041] 1) Pour 25g of EDTA dry powder into a beaker, dissolve it with a small amount of sterile water, add sterile water to 200ml after the dissolution is complete to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.
[0042] 2) Pour 65g of Tris dry powder into a beaker, dissolve it with a small amount of sterile water, add sterile water to 500ml after the dissolution is complete, to obtain a 1mol / L Tris solution.
[0043] 3) Pour 1.01 Nacl dry powder into a beaker, dissolve it with a small amount of sterile water, and add sterile water to 10ml after dissolution t...
Example Embodiment
[0046] Example 3
[0047] The preservation solution for preserving cells in human feces is characterized in that it contains 35g EDTA dry powder, 55g Tris dry powder, 0.8g NaCl dry powder in 1L of preservation solution, and the balance is sterilized water.
[0048] The method for preparing a preservation solution for exfoliated cells in human feces includes the following steps:
[0049] 1) Pour 35g of EDTA dry powder into a beaker, dissolve it with a small amount of sterile water, add sterile water to 200ml after dissolution to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.
[0050] 2) Pour 55g of Tris dry powder into a beaker, dissolve it with a small amount of sterile water, and add sterile water to 500ml after dissolution to obtain a 1mol / L Tris solution.
[0051] 3) Pour the 4.02Nacl dry powder into a beaker, dissolve it with a small amount of sterile water, add sterile water to 10ml after the dissolution is complete, and obtain a 5...
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