Use of atxn2l for preparing medicines for reversing gastric cancer oxaliplatin drug resistance

A technology of oxaliplatin and drugs, which is applied in the field of gene and protein function and application, and can solve problems affecting the efficacy of chemotherapy, etc.

Active Publication Date: 2021-11-30
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, oxaliplatin resistance often occurs, affecting the efficacy of chemotherapy

Method used

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  • Use of atxn2l for preparing medicines for reversing gastric cancer oxaliplatin drug resistance
  • Use of atxn2l for preparing medicines for reversing gastric cancer oxaliplatin drug resistance
  • Use of atxn2l for preparing medicines for reversing gastric cancer oxaliplatin drug resistance

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] 1. Experimental materials

[0014] 1. Tissue Specimens

[0015] Tissue section: Gastric cancer resection specimens were selected from Nanfang Hospital of Guangzhou Southern Medical University, including radical resection and palliative resection.

[0016] The inclusion criteria for pathological slides were: 1. Gastric cancer was confirmed by pathology; 2. Pathological slides were from the primary tumor of the stomach.

[0017] Exclusion criteria for pathological slides were: 1. Controversial pathological diagnosis; 2. Incomplete clinical treatment.

[0018] According to the patient's intraoperative condition and imaging examination, the patient was classified into TNM staging. According to the above criteria, a total of 48 cases of stage IV and 119 cases of stage I-III gastric cancer tissue samples were included for analysis. The samples were fixed with 10% formalin, embedded in paraffin, and sectioned.

[0019] Fresh gastric cancer specimens: Gastric cancer surgical...

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Abstract

The invention belongs to the field of functions and applications of genes and proteins, relates to a new application of ataxin-2-like protein (ATXN2L), and discloses the application of a drug for reversing gastric cancer oxaliplatin drug resistance. The present invention proves that the prognosis of gastric cancer patients with high ATXN2L expression is poor through clinical specimens. After silencing ATXN2L, the sensitivity of gastric cancer cells to oxaliplatin was enhanced. The oxaliplatin-resistant strain was obtained by in vitro induction, and the MTT experiment confirmed that the oxaliplatin-resistant strain was resistant to oxaliplatin and had a higher degree of malignancy. Western blot detection found that the expression of ATXN2L in oxaliplatin-resistant strains was higher than that in sensitive strains. In flow cytometry apoptosis assay, after silencing ATXN2L, the sensitivity of oxaliplatin-resistant cell lines to oxaliplatin increased. Therefore, ATXN2L can be used to prepare drugs for reversing oxaliplatin drug resistance in gastric cancer, especially inhibitors that silence ATXN2L can be used as drugs for reversing oxaliplatin drug resistance in gastric cancer.

Description

technical field [0001] The invention belongs to the field of functions and applications of genes and proteins, and relates to the new use of ataxia-2-like protein (ATXN2L), in particular to the use of ATXN2L for preparing drugs for reversing gastric cancer oxaliplatin drug resistance. Background technique [0002] Gastric cancer is a common malignant tumor in my country, and chemotherapy is an important treatment in the comprehensive treatment of gastric cancer. The resistance of tumor cells to chemotherapy drugs limits the efficacy of chemotherapy and often leads to treatment failure in patients. Chemotherapy resistance includes primary drug resistance and secondary drug resistance. Primary drug resistance refers to the tumor’s own resistance to drugs before receiving drug treatment; secondary drug resistance refers to the tumor’s original non-resistant drug resistance. Emerging drug resistance. Solving the problem of primary drug resistance and secondary drug resistance ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P35/00A61K31/555
CPCA61K31/555A61K38/17A61P35/00A61K2300/00
Inventor 廖旺军林立石敏潘常惬刘彦潭张俊浩
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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