Method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content

A technology of cytokines and freeze-dried powder, applied in the field of bioengineering, can solve the problems of no reference method, time-consuming and labor-consuming, unfavorable efficacy comparison, etc.

Pending Publication Date: 2019-04-02
陕西佰瑞衡健康科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, for the quality evaluation of these products, there is still no method for reference
If the effect is observed after directly ac...

Method used

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  • Method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content
  • Method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content
  • Method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content

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Embodiment

[0031] Example: such as figure 1 , figure 2 with image 3 As shown, a method for evaluating the hair growth effect of freeze-dried powder by cytokine content assay, its steps are as follows:

[0032] Step 1: Preparation of freeze-dried powder

[0033] 1) Primary separation of ADSCs: Obtain adipose tissue by liposuction at 1500r / min, centrifuge at room temperature for 10min, remove the liquid part, and obtain solid fat particles. Add an equal volume of saline to wash and centrifuge. Add an equal volume of 200U / ml collagenase to the solid fat particles, digest at 37°C for 60-90min with constant temperature shaking, until no fat particles are visible to the naked eye, collect the digestive juice, centrifuge at 1000r / min for 10min at room temperature, remove the supernatant, and take Add 10ml of normal saline to the cell pellet, centrifuge at 1000r / min for 5min at room temperature, wash the cells, take the cell pellet, resuspend with 3ml of culture medium, count and inoculate...

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Abstract

The invention discloses a method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content, which relates to the field of biological engineering. The method comprisesthe steps of preparation of lyophilized powder, hair follicle-related cell culture, co-culture of the lyophilized powder and hair follicle-related cells, and the collection and detection of the cellsand a supernatant after culture. The evaluation cells selected by the invention are all related to the occurrence and development of hair follicles, the method is indirectly feasible and can be usedfor evaluating the hair regeneration effect of the product in vitro, has the advantages of simple operation and strong repeatability, and can be used as the effective evaluation method for quality inspection of subsequent products.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for evaluating the hair growth effect of freeze-dried powder by measuring cytokine content. Background technique [0002] Hair loss is a worldwide skin problem, and about 50% of adult men suffer from hair loss. There are more than 100 million hair loss patients in China, and more than 90% of the hair loss population belongs to androgenetic alopecia. As people pay more and more attention to their hair problems, various hair growth techniques have also developed rapidly. [0003] Hair transplantation, as a new technology that is currently effective internationally for the treatment of hair loss, is being accepted by more and more people. However, hair transplantation must obtain healthy hair follicles from the back of the occipital, and then transplant them to the hair loss area on the forehead or top of the head. For patients with sparse hair or excessively large recipien...

Claims

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Application Information

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IPC IPC(8): C12Q1/6876G01N33/68
CPCG01N33/6863C12Q1/6876C12Q2600/158
Inventor 李晓宁明磊国董玲娟
Owner 陕西佰瑞衡健康科技有限公司
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