Wheat stripe rust resistance related protein TaNAC3 and coding gene and application thereof

A technology of related proteins and coding genes, which is applied in the field of wheat stripe rust resistance related protein TaNAC3 and its coding genes and applications, and can solve problems such as economic losses

Active Publication Date: 2019-04-16
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Wheat stripe rust is an airborne fungal disease caused by Pucciniastriiformis f.sp.tritici. It is still the

Method used

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  • Wheat stripe rust resistance related protein TaNAC3 and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. TaNAC3, an anti-stripe rust-related protein in wheat, can regulate the resistance of wheat to stripe rust

[0054] This example provides a stripe rust resistance-related protein (named TaNAC3) derived from the wheat stripe rust resistance near-isogenic line Taichung29*6 / Yr5. In the gene line Taichung29*6 / Yr5, the CDS sequence of the TaNAC3 gene is sequence 1 in the sequence listing, and positions 170-1066 of sequence 1 encode TaNAC3 protein.

[0055] Construction of gene interference vector:

[0056] Using the cDNA of the wheat stripe rust-resistant near-isogenic line Taichung29*6 / Yr5 as a template, PCR amplification was performed using primers VIGS-NAC-F1 / VIGS-NAC-R1, and the target fragment NAC3-VIGS was recovered and purified; the target fragment NAC3 -VIGS was digested with ApaI restriction endonuclease, and NAC3-VIGS digestion product was recovered. The pCaBS-γ vector was linearized with ApaI restriction endonuclease, recovered and purified to obtain t...

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Abstract

The invention discloses a wheat stripe rust resistance related protein TaNAC3 and coding gene and application thereof. The wheat stripe rust resistance related protein is A1) or A2): A1 is the proteinwith an amino acid sequence of sequence 2; A2) substitutes and/or removes and/or adds one or more amino acid residues to the amino acid shown in the sequence 2 in a sequence table and has the proteinwith the same functions of the protein shown the sequence 2. Experiments prove that the disease resistance on stripe rust of plants can be reduced after the coding genes of the stripe rust resistancerelated protein are interfered so that a transgenic plant with reduced stripe rust resistance can be obtained to be taken as a model plant for screening plant stripe rust resistance medicines. The stripe rust related protein and the genes thereof have significant meaning on culturing stripe rust resistance plants and are suitable for popularization and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to the wheat stripe rust resistance-related protein TaNAC3 and its coding gene and application. Background technique [0002] Wheat is an important food crop in China and the world. With the increase of population and the reduction of arable land, people put forward higher requirements for the yield of wheat. Wheat stripe rust is an airborne fungal disease caused by Puccinia striiformis f.sp. tritici. It is still the primary disease that threatens the safety of wheat production in my country, causing serious economic losses. Because of the high variability in virulence in stripe rust populations, cultivar resistance is often "lost" by the emergence of new endemic races. It is of practical and theoretical significance to study the long-lasting resistance of varieties and its regulation mechanism. [0003] Transcription factors can regulate the expression...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8282
Inventor 王凤涛蔺瑞明王培冯晶徐世昌
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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