Integrated double copy functional F4 pili operon gene porcine probiotic EP1 clone strain, construction method and application
A construction method and double-copy technology, applied in the field of biotechnology applications, can solve problems such as high mortality, weight loss, and slow growth of piglets, and achieve the effect of reducing the number of adhesions and improving adhesion performance
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[0027] The present invention will be described in further detail below through specific examples.
[0028] NY10 (EP1) is a non-pathogenic E. coli probiotic isolated and preserved in our laboratory, and it has been verified by whole genome sequencing. E. coli NY10 (EP1) is a non-pathogenic strain that can give pigs intestinal Health benefits, no known adverse effects on pigs were found.
[0029] In this experiment, probiotic NY10 (EP1) was used as a vaccine carrier to construct recombinant probiotics stably expressing F4 pili, which were used as daily feed additives to immunize piglets to prevent and control post-weaning diarrhea (PWD) in piglets. The F4 pilus-integrated probiotics can specifically bind to the F4 pilus receptor of porcine intestinal cells, preferentially occupy the receptor site, and induce mucosal immunity against F4 E. coli pathogens, thereby blocking F4 + Adhesion of ETEC to piglet intestines is aimed at highly effective and direct prevention of post-weanin...
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