30 results about "Post weaning diarrhea" patented technology

The invention discloses a nonreactive health powdered suckling pig creep compound feed and a preparation method thereof, wherein the feed is prepared from the following materials: corn, broken rice, puffed corn starch, puffed soybean meal, dehulling soybean meal, fermented soybean meal, white fish meal, flour, plasma protein flour, whey powder, fruit sugar, glucose monohydrate, bean oil, calcium formate, calcium biphosphate, salt, enveloped slow release acidulant, choline chloride, suckling pig compound premix, zinc oxide, yeast cell wall polysaccharides, sodium glutamate, arazyme, lipidase, Clostridium butyricum, essential oils, L-lysine hydrochloride, DL methionine, L-threonine, tryptophan and phytase. Based on the physiological characteristics of suckling pig and by the combination of various materials, the feed is supplementary in nutrition and high in design of amino acid and daily ration of energy without adding antibiotics, and Clostridium butyricum and various essential oils are used for protecting the health; therefore, the feed intake of suckling pig can be increased, the weight growth rate is increased, the diarrhea rate after ablactation is reduced, the intestine health of suckling pig can be improved, the immunity is improved, and the survival rate of suckling pig is increased.

The invention relates to a Chinese medicinal herb additive for preventing post-weaning diarrhea and a pig feed thereof of the technical field of medicaments. The additive comprises the following components in part by weight: 30 to 60 parts of pilose asiabell root, 20 to 40 parts of largehead atractylodes rhizome, 30 to 60 parts of Indian buead, 20 to 40 parts of white hyacinth bean, 30 to 60 parts of cinnamomvine, 20 to 40 parts of semen nelumbinis, 20 to 40 parts of Job stears, 10 to 30 parts of villous amomum fruit, 10 to 30 parts of costustoot, 20 to 40 parts of root of rehmannia, 20 to 40 parts of Chinese angelica, 20 to 40 parts of peony, 15 to 30 parts of Szechuan lovage rhizome, 30 to 90 parts of scorch-fried medicated leaven, 30 to 90 parts of scorch-fried malt, 30 to 60 parts of scorch-fried hawthorn fruit, 40 to 80 parts of dragon bone, 40 to 80 parts of oyster, 10 to 30 parts of platycladi seed, 10 to 30 parts of spiny jujube kernel, 10 to 30 parts of anemarrhena asphodeloides bunge, 20 to 40 parts of platycodon root, 20 to 40 parts of semen perillae acutae, 20 to 40 parts of white mulberry root-bark, 30 to 60 parts of cyrtomium rhizome, 30 to 60 parts of tuber tuber fleeceflower root, 30 to 60 parts of epimedium Herb, 20 to 40 parts of gambir plant, and 6 to 18 parts of liquoric root; and the additive accounts for 1.5 weight percent of the pig feed. The Chinese medicinal herb additive preventing the post-weaning diarrhea improves resistance to pathogenic escherichia coli of pigs.

The invention relates to a strain suitable for producing a live, orally applicableEscherichia coli vaccine for the prevention of post-weaning diarrhoea in pigs, and the procedure suitable for producing that strain. The essence of the strain is that the enterotoxin-free and originally wild-type Escherichia coli strain simultaneously produces two adhesive fimbriae (F4 and F18), whereas the essence of the procedure is that the enterotoxin-producing ability if the wild, pathogenic, enterotoxigenic Escherichia coli strain originally capable of producing enterotoxins and F18 fimbriae is abolished by a genetic intervention while retaining the ability of the strain to produce F18 fimbria facilitating adhesion to the small intestinal wall of weaned piglets, and subsequently the strain thus modified is rendered capable of producing a further surface adhesion fimbria (F4).

A preparing method of soft-pill-type fox and racoon dog pellet feed is provided. The preparing method is mainly characterized in that: fresh raw materials comprising sea trash fish, fish byproducts and livestock liver are directly smashed into a minced state. Puffed corn, puffed wheat, table salt, a microecological preparation, a composite enzyme agent, amino acids, composite premix feed, and other powder raw materials are premixed according to a ratio and smashed. The smashed dry and fresh raw materials and grease are added into a meat ball forming machine and heated for 3-5 min, and then wet balls are fished out, put onto a draining screen, aired to the room temperature and packaged into bags. The preparing method is advantageous in that: the pellet feed meets behaviour welfare of self-help feeding for foxes and racoon dogs, largely reduces other abnormal behaviors such as self-biting, cub biting and allotriophagy. According to the preparing method of the pellet feed, post-weaning diarrhea is largely reduced, disease resistance, and anti-stress capability are enhanced, the fur quality of fur animals is enhanced, guard hair is long and glossy, and the under hair is thick and soft.

The invention relates to a composite acidifier for reducing post-weaning diarrhea rate, and the composite acidifier comprises citric acid, formic acid, lactic acid and butyric acid at the mass ratio of 1:1:(0.5-0.8):0.5; and the citric acid, the formic acid, the lactic acid and the butyric acid are carried by being absorbed by silica gel. The composite acidifier can be used for increasing the daily feed intake and the daily gain of piglets, reducing the material weight ratio, saving the feeding cost and reducing the diarrhea rate of the piglets.

Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.

The invention relates to a nutritional concentrated feed for preventing post-weaning diarrhea of a piglet and enhancing the immunity. The feed comprises a main material consisting of the following raw material components in parts by weight: 50 parts of corn, 8 parts of thin rice brans, 5 parts of high-quality fish meal, 30 pars of soybean meal, 1.5 parts of calcium hydrophosphate, 0.6 part of mountain flour, 0.16 parts of salt, 0.5 parts of a transitional Chinese medicine additive, 0.1 part of vitamins and 1 part of trace elements. The nutritional concentrated feed disclosed by the invention can be used for effectively improving the disease resistance of the piglet, effectively reducing the morbidity rate of diarrhea and meeting the needs of the piglet on various nutritional ingredients in growth, is relatively low in cost and lays a firm foundation for later growth of the piglet.

The invention discloses a piglet intestine nutrition regulation and control feed based on cladded zinc oxide. The complete feed comprises 0.06-0.2wt% of cladded zinc oxide and 0.1-0.2wt% of sodium butyrate. The cladded zinc oxide is prepared by performing cladding on ingredients such as stearic acid, long-chain rice bran oil, silicate and carboxymethyl cellulose sodium by use of an inlaid cladding technology, has the effect of effectively preventing zinc oxide from being acidified into zinc ions and consuming hydrogen ions in piglet stomaches and is capable of improving the activity of pepsin, so that the active ingredient (zinc oxide) efficiently enter into an intestinal tract so as to exert effects of resisting bacteria and protecting intestinal mucosa mucoprotein structure; sodium butyrate has an effect of rapidly supplying energy nutrients to piglet intestine mucous cells, and has a food attractant effect on piglets due to unique smell. Cladded zinc oxide and sodium butyrate can achieve a synergetic effect and exert respective effects of protecting the intestinal mucosa mucoprotein structure and promoting mucosa epithelial cell growth, and the effects of improving the feed intake of weanling piglets and obviously reducing the after-weanling diarrhoea rate. The piglet intestine nutrition regulation and control feed is capable of obviously reducing the diarrhoea rate of weanling piglets and improving the feed intake and growth property of the weanling piglets.

The invention relates to a liquid amino acid and vitamin additive capable of reducing weaning stress of piglets as well as a preparation method and an application of the additive and belongs to the feed field. The liquid amino acid and vitamin additive comprises alanyl-glutamine dipeptide, sodium butyrate, decavitamin, compound amino acid, xylooligosaccharide, an emulsifier and a preservative. Theliquid amino acid and vitamin additive can increase the nutrient substance digestion rate of piglets, regulate micro-ecological balance of intestinal tracts, improve body anabolism of key nutrient substances, enhance protein synthesis, promote growth performance and increase the nursing survival rate. The preparation method of the liquid amino acid and vitamin additive comprises the steps as follows: an aqueous phase solution and an oil phase solution are prepared, respectively, the aqueous phase and the oil phase solution are mixed, and the liquid amino acid and vitamin additive is prepared.The preparation method is simple and easy to operate. After the drink is used for preparing piglet feed, the feed intake of weaned piglets can be increased, the daily weight gain can be increased, diarrhea rate after weaning can be decreased, and weaning stress of the weaned piglets can be relieved.

The invention relates to a Chinese medicinal herb additive for preventing post-weaning diarrhea and a pig feed thereof of the technical field of medicaments. The additive comprises the following components in part by weight: 30 to 60 parts of pilose asiabell root, 20 to 40 parts of largehead atractylodes rhizome, 30 to 60 parts of Indian buead, 20 to 40 parts of white hyacinth bean, 30 to 60 parts of cinnamomvine, 20 to 40 parts of semen nelumbinis, 20 to 40 parts of Job stears, 10 to 30 parts of villous amomum fruit, 10 to 30 parts of costustoot, 20 to 40 parts of root of rehmannia, 20 to 40parts of Chinese angelica, 20 to 40 parts of peony, 15 to 30 parts of Szechuan lovage rhizome, 30 to 90 parts of scorch-fried medicated leaven, 30 to 90 parts of scorch-fried malt, 30 to 60 parts of scorch-fried hawthorn fruit, 40 to 80 parts of dragon bone, 40 to 80 parts of oyster, 10 to 30 parts of platycladi seed, 10 to 30 parts of spiny jujube kernel, 10 to 30 parts of anemarrhena asphodeloides bunge, 20 to 40 parts of platycodon root, 20 to 40 parts of semen perillae acutae, 20 to 40 parts of white mulberry root-bark, 30 to 60 parts of cyrtomium rhizome, 30 to 60 parts of tuber tuber fleeceflower root, 30 to 60 parts of epimedium Herb, 20 to 40 parts of gambir plant, and 6 to 18 parts of liquoric root; and the additive accounts for 1.5 weight percent of the pig feed. The Chinese medicinal herb additive preventing the post-weaning diarrhea improves resistance to pathogenic escherichia coli of pigs.

The invention discloses low-zinc antibiotic-free high-protein creep feed beneficial to improving diarrhea of weaned piglets. The creep feed is prepared by mixing the following components by weight: first-grade rice, puffed corns, high-protein whey powder, soybean oil, puffed peeled soybean meal, puffed soybeans, super steam fish meal, chicken plasma protein powder, whey protein concentrate, stonepowder, calcium hydrophosphate, sodium chloride, vitamin premix for suckling pigs, trace element premix for the suckling pigs, choline chloride, arginine, lysine, methionine, coated zinc oxide, antimicrobial peptide, lysozyme, acidulant, tannic acid, plant soot, butyrin, coated sodium butyrate, prebiotics, probiotics, acid protease, mannase, phytase, mildew preventive, antioxidant and flour. The creep feed disclosed by the invention can solve the problems of incomplete immune function, weak disease resistance, insufficient digestive enzyme secretion, incomplete digestive tract development, poor absorption capacity, easy diarrhea, slow growth and the like of the weaned piglets.

The invention provides a concentrate supplement for early weaning of lambs of Guanzhong dairy goats, and belongs to the technical field of feed. The concentrate supplement is prepared from the following components in parts by weight: 50-62 parts of puffed corn, 2.5-4.0 parts of wheat bran, 13.6-18.5 parts of puffed soybeans, 4.5-5.5 parts of rice, 5.0-8.0 parts of whey powder, 5.0-6.0 parts of fermented soybean meal, 0.2-0.5 part of sodium chloride, 0.1-0.3 part of sodium bicarbonate, 1.3-1.8 parts of lysine, 0.5-0.8 part of methionine, 3.0-4.5 parts of a Chinese herbal medicine mixture, 0.01-0.02 part of an antioxidant, 0.02-0.06 part of a mildew-proof agent, 0.01-0.03 part of a flavoring agent, 0.01-0.02 part of a trace element additive and 0.01-0.02 part of multivitamins. The inventionfurther provides a preparation method of the concentrate supplement. By adding the puffed raw materials, anti-nutrition factors in the corn and the soybeans are reduced, and digestion and absorption of nutrients are improved; the fermented soybean meal serves as a carrier, specific microbial floras are guided in rumina of the lambs, and thus rumen inflammation is avoided; and through the Chinese herbal medicine mixture, gastrointestinal development of the early weaning lambs is adjusted and controlled, the resistance of the lambs after weaning is enhanced, diarrhea after weaning is reduced, and thus the survival rate of the lambs is increased.

The invention provides a premix for relieving post-weaning diarrhea. The premix is prepared from the following raw materials in parts by weight: 15-25 parts of agastache rugosa, 5-15 parts of pericarpium citri reticulatae, 10-30 parts of coptis chinensis, 5-10 parts of semen plantaginis, 5-15 parts of lonicera japonica, 10-30 parts of yam extract, 10-30 parts of tikoua fig root, 3-8 parts of licorice root, 5-20 parts of neomycin and 2-5 parts of charcoal. The premix is capable of relieving post-weaning diarrhea.

The invention relates to a post-weaning piglet nutritional diarrhea model, specifically relates to the high-casein ration-induced type post-weaning piglet nutritional diarrhea model, and belongs to the field of establishment of animal models. A establishing method of the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is characterized in that a 21-day post-weaning piglet is continuously fed for a high-casein ration for 15 days, casein in the high-casein ration is a unique source of protein, the adding amount of the casein is 33.72%, and the content of crude protein in the ration is 30%; and after a test is finished, a piglet diarrhea index, a serum biochemical index, an intestinal structure form and the expression amounts of an intestinal water channel protein marker molecule and a tight junction protein marker molecule in the piglet are measured. The establishing method involved in the invention has the beneficial effects that the casein is used as the unique source of the protein, the adding amount of the casein in the ration is determined, and the high-casein ration-induced type post-weaning piglet nutritional diarrhea model is established, so thata reliable model is provided for the deep exploration and prevention of post-weaning diarrhea of newborn animals.

Antibiotics have long been used at sub-therapeutic levels to control incidences of post-weaning diarrhea and to improve growth performance in pigs. However, the current trend world-wide is to eliminate the use of in-feed antibiotics due to increased public concerns over the spread of antibiotic resistance in bacterial pathogens, which poses a threat to public health. Alternatives to in-feed antibiotics are needed. Thymol essential oil exhibits strong in vitro antibacterial activity; however; direct inclusion of essential oils to pig feeds has limited efficacy due to their high volatility, low stability during feed processing, interactions with other feed components and poor availability in lower gut. To solve these problems, we developed lipid matrix beads using thymol and a fatty acid with incorporation of 2% polysaccharides via a melt-granulation technique. Laurie acid was identified as a suitable carrier for thymol. In vitro release of thymol from lipid matrix granules was determined using simulated salivary fluid (SSF), simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), respectively. The lipid matrix granules with 2% polysaccharides exhibited a slow release rate (%) of essential oil and fatty acid in SSF (21.2±2.3; 36±1.1), SGF (73.7±6.9; 54.8±1.7) and SIF (99.1±1.2; 99.1±0.6), respectively. However, the lipid matrix granules without polysaccharides had quick release (%) of essential oil and fatty acid from the SSF (79.9±11.8; 84.9±9.4), SGF (92.5±3.5; 75.8±5.9) and SIF (93.3±9.4; 93.3±4.6), respectively.

The invention relates to an artificial feed, in particular to an artificial feed containing cinnamyl aldehyde, probiotics and complex enzymes. The artificial feed is characterized in that the cinnamyl aldehyde accounts for 0.01% of the total components of the feed; the probiotics account for 1% of the total components of the complete feed and 1 g of the probiotics comprise 20 billion viable bacillus subtilis, 20 billion viable lactic acid bacteria and 20 billion yeasts; the complex enzymes account for 0.1% of the total components of the complete feed and comprise 3000 IU / g of xylanase, 4000 IU / g of protease and 2000 IU / g of amylase; a preparation process comprises the steps of performing secondary granulation at low and high temperatures. By adding the cinnamyl aldehyde, the probiotics and the complex enzymes into the artificial feed, the post-weaning diarrhea can be relieved, the intestinal digestion function and the immunity can be improved, the feed utilization rate can be increased, and the feed conversion ratio can be reduced; in addition, the artificial feed has good feeding effect and economic benefit.

The invention provides pre-mixed feed for piglets. The pre-mixed feed is mainly characterized by being made by crushing corn and soybean meal, feeding into a raw material cabin, and mixing with various vitamins according to a ratio. The pre-mixed feed specifically comprises the following components in parts by weight: 750-800g of corn, 80-100g of soybean meal, 75-95g of fish flour, 25-30 ten thousands IU of nucleotide, amino acid, medium-chain fatty acid and vitamin A, 2-4.9 ten thousand IU of vitamin D3, 25-35mg of vitamin K3, 250-300mg of vitamin B1, 250-300mg of vitamin B2, 250-300mg of vitamin B6, 1-1.4mg of vitamin B12, 7.5-10.5mg of D-vitamin, 500-650mg of nicotinic acid, 15-19.5mg of folic acid, 4500-5850mg of vitamin C, 0.12-3g of copper, 0.8-18.7g of iron, 1.5-3.7g of zinc, 0.1-3.7g of manganese, 5-245mg of iodine and 2.5-12.4mg of selenium. The pre-mixed feed has the advantages that weaning stress can be reduced, the food intake amount can be rapidly increased after weaning,loss of flesh can be avoided, and the weight can be increased. After weaning, diarrhea can be barely caused, intestinal health can be achieved, the content of hemoglobin of piglets can be increased, and the content of hemoglobin is greater than 110-125g / L. The immunity of the piglets can be improved, and the pre-mixed feed is complete and uniform in nutrition component.

The invention relates to thefield of biotechnology, in particular to an integrated double copy functional F4 pili operon gene porcine probiotic EP1 clone strain, a construction method and application.The method comprises the following steps that pTargetT-maeB::PtetF4 and pTargetT-nth / tppB::PtetF4recombinant plasmids are constructed; a non-resistant doublecopy F4 pili geneintegrated clone strain is obtained by constructing the probiotic clone strain of an integrated doublecopy functional F4 pili operon gene. The probiotic clone strain is used as a probiotic live vaccine candidate strain and atherapeutic drug for post-weaning diarrhea and an edema disease in piglets. Compared with the prior art, the strain has the advantages that a porcine probiotic NY10 (EP1) and a non-resistant gene exist, recombinant bacteria can effectively improve the adhesion performance to intestinal passage cells of pigs, cell infection inhibition experiments in vitroshow that pre-incubationrecombinant bacteriaand IPEC-J2 cells can competitively inhibit the infection of F4 piluspositive E. coli pathogen 3030-2 against the IPEC-J2 cells, and the number of adhesion is significantly reduced.

The invention provides a premix for relieving post-weaning diarrhea. The premix is prepared from the following raw materials in parts by weight: 15-25 parts of agastache rugosa, 5-15 parts of pericarpium citri reticulatae, 10-30 parts of coptis chinensis, 5-10 parts of semen plantaginis, 5-15 parts of lonicera japonica, 10-30 parts of yam extract, 10-30 parts of tikoua fig root, 3-8 parts of licorice root, 5-20 parts of neomycin and 2-5 parts of charcoal. The premix is capable of relieving post-weaning diarrhea.

The invention relates to a preparation method of a livestock feed premix as well as a product and application thereof. Feed contains calcium glycinate with the mass fraction of above 0.5%; with adoption of calcium glycinate, the post-weaning diarrhea rate of piglets is reduced, the diarrhea period is shortened, and the daily gain of the piglets is improved after the piglets are weaned for 10-20 days; meanwhile, the digestive ability of proteins of the piglets is promoted, and the immunity of the weaned piglets is improved. Therefore, the product has an important meaning for improving the performance of the weaned piglets.

A Lactobacillus paracasei fermented feed and its preparation method, fermentation equipment and application. The preparation method includes the steps of liquid fermentation of Lactobacillus paracasei and solid fermentation of Lactobacillus paracasei. High efficiency, low energy consumption and other advantages. The fermentation equipment includes a support seat, a fermentation tank and a stirring assembly installed in the fermentation tank. The fermentation tank includes a tank body with a material inlet and outlet and a sealing cover for sealing the material inlet and outlet. The sealing cover is movably connected with the tank body, and the tank body can be The swinging device is installed on the support seat, and the support seat is equipped with a swing drive assembly for driving the tank to tilt and discharge. It has the advantages of compact structure, small footprint, simple operation, beautiful appearance, convenient cleaning, good mixing effect, and can greatly reduce the Labor intensity and other advantages. The Lactobacillus paracasei fermented feed and its use have the advantages of being able to significantly reduce the incidence of post-weaning diarrhea in piglets, increase the growth rate of pigs, and improve the efficiency of feed conversion and utilization.

The invention relates to a method for cultivating high-yield disease-resistant new-strain Meishan pigs. The method is characterized by including: infecting a Meishan pig core group with F18 escherichia coli, and forming a Meishan pig F18 escherichia coli resistance breeding underlying group according to results of phenotype identification; measuring the concentration of F18 escherichia coli resistance group cell factors (IL-2, IL-6, IL-10, IL-12, IFN-alpha and IFN-gamma) in the underlying group; performing comprehensive index breeding on common disease resistance according to individual comprehensive scores by building a main component comprehensive evaluation model, and detecting and selecting characters such as reproductive performance at the same time to obtain a first generation; using continuous progeny selection and molecular marker-assisted selection to gradually achieve the breeding target of cultivating the new strain core group after 4-5 generations. The new-strain pigs are resistant to post-weaning diarrhea, edema diseases and stress, high in environment adaptability, good in meat quality, high in feed conversion and large in litter size.

The present invention provides non-invasive methods and compositions to differentiate, with a high level of sensitivity and specificity, swine that are genetically susceptible to diseases associated with F18 E. coli infection, from resistant swine. DNA polymorphisms in the swine alpha (1,2) fucosyltransferase 1 (FUT1) gene were used to differentiate resistant from susceptible swine. The invention includes a polypeptide with amino acid substitutions, encoded by the nucleotide polymorphisms, a molecular diagnostic assay, and a kit for the differentiation, of E. coli F18-adhesion resistant, heterozygous (carrier) and homozygous susceptible pigs. The molecular test identifies susceptibility to oedema disease and postweaning diarrhea with high sensitivity and specificity, therefore, is useful to swine breeder in their effort to enhance for resistance. Information on the polymorphisms of the present invention provides insight into causation and treatment of E. coli associated intestinal disorders.

The invention discloses a baked wheat feed for piglets and a preparation method thereof, and relates to the field of pig feed. The feed for piglets comprises the following raw materials in percentageby weight: 62.0% of wheat, 9.2% of soybean meal, 4.0% of plasma protein powder, 5% of fish meal, 15% of whey powder, 0.5% of L-lysine hydrochloride, 0.1%of threonine, 1.8% of soybean oil, 0.1% of calcium hydrogen phosphate, 0.3% of stone powder, 1.0% of citric acid and 1.0% of a composite pre-mixed feed. The preparation method comprises the following steps: weighing the raw materials in percentageby weight, crushing the wheat, adding other raw materials into the crushed wheat, and conducting uniform mixing; or conducting second granulation after the uniform mixing. The feed of the invention is effective in reducing post-weaning diarrhea (PWD) of piglets during the first 2 weeks after weaning, especially during the first 10 days after the weaning, and can improve the production performanceof the piglets, reduce feed cost, provide new nutrients for intestinal tracts, and improve intestinal health.

The present invention relates to the field of biotechnology, in particular to probiotic clones, construction methods and applications that integrate a single-copy functional F4 pilus operon gene, comprising the following steps: pTargetT- nth / wxya ::P tet Construction of F4 recombinant plasmid; through the construction of a probiotic clone strain integrating a single copy of the functional F4 pili operon gene, a non-resistant single copy F4 pili gene integrated clone is obtained. The probiotic cloning strain is used as a probiotic live vaccine candidate strain and a drug for treating post-weaning diarrhea and edema disease of piglets. Compared with the prior art, the beneficial effects of the present invention are as follows: the original plasmid of the Nissle1917 probiotic is removed, and there is no resistance gene; the recombinant bacterium can effectively improve the adhesion performance to pig intestinal passage epithelial cells; orally immunize mice It can produce immune serum, that is, IgG antibody against F4 pili; the serum of mice after oral immunization can significantly reduce F4 + Adhesion of strains to a porcine intestinal passage cell line.

The invention discloses antibiotic-free diarrhea-relieving special premixed feed for suckling piglets. The feed comprises soybean meal, sodium chloride, stone powder, a vitamin complex, compound traceelements, fructooligosaccharide, phytase, calcium dihydrogen phosphate, a flavoring agent, a sweetening agent, a bilberry extract, a shiny-leaved yellowhorn shell extract, mulberry leaf flavonoids and fermented rapeseed dregs, wherein the bilberry extract and the shiny-leaved yellowhorn shell extract are beneficial to maintaining the body health of the pigs, and have higher antioxidant capacity;the mulberry leaf flavonoids increase the absorption and utilization rate of the feed; and the fermented rapeseed dregs reduce the overall cost of the feed, improve the palatability of the feed, relieve post-weaning diarrhea and improve the digestibility of nutrient substances. After 540 weaned pigs are continuously fed with the premixed feed, it is discovered that the diarrhea rate of the weanedpigs drops to 3.7%-4%, the average daily weight gain is 313-347g, the average daily feed intake is 558-574g, and the utilization rate of the feed and the production performance of the weaned pigs areimproved.

A preparing method of soft-pill-type fox and racoon dog pellet feed is provided. The preparing method is mainly characterized in that: fresh raw materials comprising sea trash fish, fish byproducts and livestock liver are directly smashed into a minced state. Puffed corn, puffed wheat, table salt, a microecological preparation, a composite enzyme agent, amino acids, composite premix feed, and other powder raw materials are premixed according to a ratio and smashed. The smashed dry and fresh raw materials and grease are added into a meat ball forming machine and heated for 3-5 min, and then wet balls are fished out, put onto a draining screen, aired to the room temperature and packaged into bags. The preparing method is advantageous in that: the pellet feed meets behaviour welfare of self-help feeding for foxes and racoon dogs, largely reduces other abnormal behaviors such as self-biting, cub biting and allotriophagy. According to the preparing method of the pellet feed, post-weaning diarrhea is largely reduced, disease resistance, and anti-stress capability are enhanced, the fur quality of fur animals is enhanced, guard hair is long and glossy, and the under hair is thick and soft.

The present invention provides non-invasive methods and compositions to differentiate, with a high level of sensitivity and specificity, swine that are genetically susceptible to diseases associated with F18 E. coli infection, from resistant swine. DNA polymorphisms in the swine alpha (1,2) fucosyltransferase 1 (FUT1) gene were used to differentiate resistant from susceptible swine. The invention includes a polypeptide with amino acid substitutions, encoded by the nucleotide polymorphisms, a molecular diagnostic assay, and a kit for the differentiation, of E. coli F18-adhesion resistant, heterozygous (carrier) and homozygous susceptible pigs. The molecular test identifies susceptibility to oedema disease and postweaning diarrhea with high sensitivity and specificity, therefore, is useful to swine breeder in their effort to enhance for resistance. Information on the polymorphisms of the present invention provides insight into causation and treatment of E. coli associated intestinal disorders.

Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.