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Breeding method of high-yield and disease-resistant new line of Meishan pig

A high-yield, disease-resistant, and breeding method technology, which is applied in animal husbandry and other fields, can solve problems such as the conception rate of sows, the decrease in the survival rate of weaned piglets born alive, the inability to fully exert the high fecundity of Meishan pigs, and the decline in disease resistance, etc. problem, to achieve the effect of improving general disease resistance, improving immune response ability, and meat quality

Active Publication Date: 2016-10-26
YANGZHOU UNIV
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Problems solved by technology

The excellent characteristics of Meishan pigs are mainly high reproductive performance and excellent meat quality, but there are also problems and deficiencies such as low lean meat rate and decreased disease resistance, especially in recent years, the incidence of diseases such as diarrhea, edema and asthma in piglets is relatively high. High, directly and indirectly lead to a decline in the conception rate of sows, the number of live piglets and the survival rate of weaned piglets, so that the recognized high fecundity of Meishan pigs cannot be fully utilized

Method used

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  • Breeding method of high-yield and disease-resistant new line of Meishan pig
  • Breeding method of high-yield and disease-resistant new line of Meishan pig
  • Breeding method of high-yield and disease-resistant new line of Meishan pig

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Embodiment

[0027] (1) Carry out F18 Escherichia coli (purchased from the China Veterinary Drug Administration) infection test on the core group of Meishan pigs by adding feed, according to the results of phenotypic identification, set up the basic group of Meishan pigs F18 Escherichia coli disease-resistant breeding;

[0028] 1) Bacterial culture Inoculate a single colony of F18 Escherichia coli into 3-5 mL of LB medium, culture it with shaking at 37°C overnight (about 18 hours), wash the bacteria with PBS repeatedly for 3 times the next day, and inoculate the Escherichia coli strain Dilute with PBS at a ratio of 1:20, and adjust the bacterial concentration to 4.6×10 8 CFU / L.

[0029] 2) Feed preparation According to the nutritional requirements of weaned piglets, feed pellets containing 21.7% crude protein. The feed does not contain any antibiotics and probiotics. The main purpose of preparing feed that does not contain antibiotics and probiotics is to avoid antibiotics in the feed. In...

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Abstract

The invention relates to a method for cultivating high-yield disease-resistant new-strain Meishan pigs. The method is characterized by including: infecting a Meishan pig core group with F18 escherichia coli, and forming a Meishan pig F18 escherichia coli resistance breeding underlying group according to results of phenotype identification; measuring the concentration of F18 escherichia coli resistance group cell factors (IL-2, IL-6, IL-10, IL-12, IFN-alpha and IFN-gamma) in the underlying group; performing comprehensive index breeding on common disease resistance according to individual comprehensive scores by building a main component comprehensive evaluation model, and detecting and selecting characters such as reproductive performance at the same time to obtain a first generation; using continuous progeny selection and molecular marker-assisted selection to gradually achieve the breeding target of cultivating the new strain core group after 4-5 generations. The new-strain pigs are resistant to post-weaning diarrhea, edema diseases and stress, high in environment adaptability, good in meat quality, high in feed conversion and large in litter size.

Description

technical field [0001] The invention relates to a method for cultivating a new high-yield and disease-resistant strain of Meishan pig, and belongs to the technical fields of animal genetics, breeding and reproduction, and molecular biology. Background technique [0002] my country's pig industry has a strong pig production system, which is often said that "my country is a big country for pig farming", but the top core breeding pig resources in the "pyramid" breeding system have long relied on imports. For a long period of time, my country has been in a vicious circle of "introduction → maintenance → degradation → re-introduction" for breeding pigs. After joining the WTO, this situation has become more serious. In short, the obvious dependence on the introduction of breeding pigs is very unfavorable to the sustainable development of my country's pig industry. Considering the long-term interests of the country, this phenomenon should not continue to develop. Faced with this s...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
Inventor 包文斌赵乔辉朱世平吴圣龙朱国强孙寿永华金第
Owner YANGZHOU UNIV
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