Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular identifier related to laying duck egg laying and egg quality traits and application thereof

A technology for molecular markers and egg quality, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems that there is no molecular marker of LRRTM1 gene in laying ducks.

Active Publication Date: 2019-04-16
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the study of LRRTM1 gene in laying ducks. So far, there is no research on the use of LRRTM1 gene in laying ducks as a molecular marker for duck laying and egg quality traits.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular identifier related to laying duck egg laying and egg quality traits and application thereof
  • Molecular identifier related to laying duck egg laying and egg quality traits and application thereof
  • Molecular identifier related to laying duck egg laying and egg quality traits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Obtaining the Partial Coding Region Fragment of Laying Duck LRRTM1 Gene and Establishment of SNP Detection Method

[0023] 1. Extraction of genomic DNA from laying ducks

[0024] The test laying duck variety of the present invention is a new strain of Jingjiang laying duck, and the samples are from the poultry testing field of the Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences. The genomic DNA of laying ducks was extracted using a genomic DNA kit produced by Beijing Biotech Biotechnology Co., Ltd. (operated according to the kit instructions). The specific steps are as follows:

[0025] (1) Use a disposable syringe to extract about 1ml of blood from the vein below the duck wing, inject it into a 1.5ml centrifuge tube that has been sterilized by high pressure and filled with 200 μL of sterile ACD anticoagulant, shake it gently, and record the wing number, - Store at 20°C for later use. Aspirate 10 μL of anti...

Embodiment 2

[0047] Example 2 Detection of polymorphism distribution of molecular markers prepared by the present invention in Jingjiang laying ducks

[0048] The polymorphism of the T1259C site in the partial coding region of the laying duck LRRTM1 gene was detected, and three genotypes were detected in the tested population. The genotype frequency and distribution are shown in Table 3.

[0049] Table 3 Genotype frequency and allele frequency of LRRTM1 gene in Jingjiang laying duck

[0050]

[0051] Remarks: In the column of genotype frequency, the genotype frequency is in the brackets, and the number of individuals of the genotype is outside the brackets.

[0052] It can be seen from Table 3 that the base polymorphism site of T or C at the 1259th bp of the sequence shows three genotypes of TT, TC or CC, wherein the TC genotype is the dominant genotype.

Embodiment 3

[0053] Example 3 Correlation analysis and application of molecular markers of the present invention and egg production and egg quality traits of laying ducks

[0054] In order to determine whether the SNP in the partial coding region of the LRRTM1 gene of laying ducks is related to the differences in laying and egg quality traits of laying ducks, a new line of Jingjiang laying ducks (656 samples) was selected as the test material, and the sample collection and data collection were carried out in conjunction with The Poultry Experimental Field of the Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences, used direct sequencing to detect polymorphisms, and analyzed the relationship between different genotypes in the partial coding region of the LRRTM1 gene of laying ducks and the egg production and egg quality traits of laying ducks. The association analysis between genotype and phenotype was carried out by SPSS 18.0 software, and the model...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a molecular identifier related to laying duck egg laying and egg quality traits and application thereof. The molecular identifier is located in part of coding area sequence of alaying duck LRRTM1 gene, the nucleotide sequence of the molecular identifier is as shown in SEQ ID NO:1, the length of the sequence is 1427 bp, and there exists T / C biallelic mutation at the 1259 bp of the sequence; the single nueleotide polymorphism (SNP) of the sequence can be taken as the related molecular identifier of the egg laying duck egg laying and egg quality related trait correlation analysis. The invention further discloses a screening method of the molecular identifier and the application of the molecular identifier in the laying duck egg laying and egg quality related traitcorrelation analysis. The application of the molecular identifier in laying duck egg laying and egg quality trait auxiliary selection has the advantages that a detection method is rapid and accurateand not influenced by the condition factors of the culturing environment.

Description

technical field [0001] The invention relates to the technical field of screening molecular markers of laying ducks, in particular to molecular markers related to egg production and egg quality traits of laying ducks and applications thereof. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphsim, SNP) refers to the polymorphism caused by the mutation of a single nucleotide (A, T, C or G) in the genomic DNA sequence, including the insertion of a single base, Deletions, transformations, and transversions, etc. Due to its wide distribution and stability, it plays an important role in animal molecular marker-assisted selection (MAS) breeding, and has become a feasible technology to accelerate the genetic improvement of important economic traits of laying ducks, especially those limiting traits. Based on this, further identification of molecular markers related to the economic traits of laying ducks, and then the use of MAS will greatly impr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴艳肖红卫张昊梁振华皮劲松潘爱銮蒲跃进申杰孙静陈颖杜金平
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com