Exosome protein for osteosarcoma diagnosis and instant detecting method thereof

A technology of exosomes and osteosarcoma, applied in the field of biomarkers, can solve the problem of lack of biomarkers of chronic suppurative osteomyelitis, metastatic bone tumors and osteosarcoma, and achieve low cost, simple operation, and convenient detection Effect

Active Publication Date: 2019-04-19
杭州多泰科技有限公司
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AI Technical Summary

Problems solved by technology

In clinical diagnosis, it needs to be differentiated from chronic suppurative osteomyelitis, Ewing's sarcoma, metastatic bone tumor, and bone and joint tuberculosis. tumor biomarkers

Method used

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  • Exosome protein for osteosarcoma diagnosis and instant detecting method thereof
  • Exosome protein for osteosarcoma diagnosis and instant detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening of osteosarcoma exosome protein markers

[0033] 1. Selection, isolation and identification of serum exosome samples

[0034] In order to study the difference between osteosarcoma and chronic suppurative osteomyelitis, Ewing's sarcoma, metastatic bone tumor, bone and joint tuberculosis, we selected 32 cases of osteosarcoma, 30 cases of chronic suppurative osteomyelitis, 29 cases of Ewing's sarcoma, Extracellular vesicles (EVs) were isolated from the serum samples of 30 cases of metastatic osteosarcoma, 28 cases of bone and joint tuberculosis and 32 normal people as the research object.

[0035] The isolated EVs were shown to be exosomes using nanoparticle tracking analysis (NTA), circular morphology (transmission electron microscopy), size, and markers (immunoblot for CD9, CD63, and CD81).

[0036] 2. Proteomic screening of osteosarcoma markers

[0037] Differentially expressed proteins in exosomes of pathological samples were identified by proteom...

Embodiment 2

[0050] Example 2 Expanded sample verification of the diagnostic value of C19ORF43 for osteosarcoma

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Abstract

The invention discloses use of a reagent for detecting the expression level of a C19orf43 protein from a serum exosome as a marker for diagnosing osteosarcoma. The maker can realize reliable diagnosis, particularly realizes distinguishing of osteosarcoma, chronic pyogenic osteomyelitis, Ewing's sarcoma, metastatic tumor of bone and osteoarticular tuberculosis. Furthermore the invention provides aninstant detecting platform of the exosome protein for detecting the marker. The method has advantages of high efficiency, simple operation, low cost and high clinical auxiliary diagnosis value.

Description

technical field [0001] The invention relates to the field of biomarkers, in particular to an exosome protein used for the diagnosis of osteosarcoma and a real-time detection method thereof. Background technique [0002] Osteosarcoma is a malignant tumor in which tumor cells can directly form neoplastic bone-like tissue or bone tissue, accounting for about 20-34% of all primary malignant bone tumors, and is the most common malignant primary bone tumor. Osteosarcoma is a highly malignant tumor with a slightly higher incidence in men. It can occur at all ages, but it is most common in 11-20 years old, followed by 21-30 years old. The older the age, the lower the incidence rate. Osteosarcoma mostly occurs in the vigorous period of bone growth and development, and its malignancy is relatively high. Therefore, it is one of the important tumors that seriously affect labor productivity and endanger life. Early diagnosis and early treatment are of special significance. In clinical d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558
CPCG01N33/543G01N33/558
Inventor 王国胜
Owner 杭州多泰科技有限公司
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