A kind of islet cell microcapsule and its preparation method and application

A technology of islet cells and microcapsules is applied in the field of microcapsules encapsulating islet cell clusters and their preparation, and can solve problems such as immune rejection.

Active Publication Date: 2020-01-10
HUNAN XENO LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The primary purpose of the present invention is to provide a microcapsule prepared by using mesenchymal stem cells around the islet cell mass to address the problems of immune rejection, hypoxia, starvation and the like in the current islet cell transplantation process

Method used

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  • A kind of islet cell microcapsule and its preparation method and application
  • A kind of islet cell microcapsule and its preparation method and application
  • A kind of islet cell microcapsule and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Preparation of porcine islet cell cluster microcapsules and porcine islet cell microcapsules wrapped with porcine mesenchymal stem cells of the present invention

[0046]Porcine islet cell count: 50 μl samples were taken out of the porcine islet cell suspension each time, DTZ-positive cell clusters were counted under the microscope and the sampling was repeated 3 times, and the islets were counted according to the following formula: islet yield=(sum of 3 positive islet values / 3 )×[Total amount of sample (ml) / 50μl].

[0047] Evenly distribute a portion of 10000IEQ islet cell clusters in 1ml of 2% sodium alginate solution, drop the suspension of islet cell sodium alginate into 50ml of 100mM calcium chloride solution with a 27G syringe needle at a speed of 2-3min per ml, and form Porcine islet microcapsules with a diameter of about 300 μm, collected porcine islet cell mass microcapsules and 5×10 6 Mix a porcine mesenchymal stem cell with 1ml of 2% sodium alginate solution...

Embodiment 2

[0050] Example 2 Transplantation experiment of porcine islet cell microcapsules and porcine mesenchymal stem cells encapsulating porcine islet cell mass microcapsules in diabetic mice

[0051] Porcine islet microcapsules and porcine mesenchymal stem cell-wrapped porcine islet cell mass microcapsules were prepared according to the method described in Example 1, and were transplanted into the peritoneal cavity of diabetic nude mice respectively. There were 5 mice in each group, and each mouse was transplanted according to the amount of 2000IEQ porcine islet cells. Blood glucose of mice was monitored every 2 days for one month. Recover the microcapsules and do DTZ staining. Figure 4 The experimental results showed that porcine islet cell microcapsules wrapped with porcine mesenchymal stem cells could recover functional islet cell clusters after one month, and DZT staining could stain porcine islet cells in scarlet, while islet cells in porcine islet microcapsules Most of the c...

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Abstract

The invention belongs to the technical field of islet cell graft preparation, and particularly relates to an islet cell microcapsule, and a preparation method and an application of the islet cell microcapsule. The microcapsule is prepared by wrapping a porcine islet cell cluster in a central position, and then wrapping the porcine islet cell cluster with porcine mesenchymal stem cells. The microcapsule having porcine islet cells wrapped with the porcine mesenchymal stem cells can allow the porcine islet cell cluster to withstand the problems of oxygen deficit and lack of nutritional factors ina transplanting process, and also reduce an immunological rejection effect of a receptor on a porcine pancreatic island after the porcine islet cells are transplanted. Through a technology of the islet cell microcapsule, the porcine pancreatic island can be transplanted in an abdominal cavity or under omentum majus to secrete an appropriate amount of insulin to treat a patient with diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of islet cell transplant preparation, and relates to a microcapsule for wrapping islet cell clusters, a preparation method and application thereof. Background technique [0002] Diabetes is a common complex metabolic disease. According to the latest report, about 425 million adults worldwide suffer from diabetes in 2017, with an average of 1 in every 11 people suffering from diabetes. Among them, the number of patients in China reached 114 million, and the burden of diabetes was the heaviest. Insulin injection is currently an effective means of controlling diabetes, but exogenous insulin cannot achieve physiological regulation of blood sugar, so it is difficult to improve diabetic complications, which greatly reduces the quality of life of patients and eventually leads to death. [0003] Studies have found that islet replacement therapy, that is, pancreas (pancreatic islet) transplantation, is currently one...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/36A61K47/02A61K35/39A61P3/10A61K35/28
Inventor 王维
Owner HUNAN XENO LIFE SCI
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