Positive feedback amplification electrochemical sensor based on exonuclease (Exo) III

An exonuclease and electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, biochemical equipment and methods, etc., can solve the problems of increased design complexity and increased cost, and achieve high selectivity and ultra-high sensitivity , has a selective effect

Active Publication Date: 2019-05-10
SHANDONG UNIV
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  • Application Information

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Problems solved by technology

However, the nicking enzyme has a special recognition site, so it puts forward higher requirements on the DNA sequence, re...

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  • Positive feedback amplification electrochemical sensor based on exonuclease (Exo) III
  • Positive feedback amplification electrochemical sensor based on exonuclease (Exo) III
  • Positive feedback amplification electrochemical sensor based on exonuclease (Exo) III

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[0037] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0038] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0039] Such as figure 1 As shown, a novel DNA electrochemical sensor based on Exo III positive feedback amplifi...

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Abstract

The invention discloses a positive feedback amplification electrochemical sensor based on exonuclease (Exo) III. The positive feedback amplification electrochemical sensor comprises a gold electrode and Hybrid DNA fixed to the gold electrode; the Hybrid DNA sequentially comprises a sulfur-hydrogen bond, a double-stranded DNA structure and a single-stranded DNA structure from the 5' end to the 3' end; the double-stranded DNA structure is prepared from C-DNA and A-DNA as well as G4-DNA which are hybridized; the basic group sequence of the single-stranded DNA structure is complementary with the basic group sequence of T-DNA; and the Hybrid DNA is fixed to the gold electrode through an S-Au key. Through Hairpin DNA and the Hybrid DNA which are ingeniously designed, and target object circular amplification based on the Exo III, a positive feedback amplification strategy combining a homogeneous phase reaction with a heterogeneous phase reaction is achieved; and the strategy shows ultra-highsensitivity, the detection limit is as low as 0.12 aM T-DNA, and high selectivity for the T-DNA and other base group mismatch DNA is achieved.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, in particular to a positive feedback amplification electrochemical sensor based on exonuclease III and its preparation method and application. Background technique [0002] Acquired immune syndrome, also known as AIDS, is an acquired infectious disease. When the human immunodeficiency virus (HIV) was first identified in the United States in 1983, it was accompanied by one of the world's deadliest diseases, acquired immunodeficiency syndrome (AIDS). The genes of this HIV group are two identical strands of RNA that, through reverse transcription, can be transcribed into DNA for further gene expression in the host cell. It attacks the host's immune system and causes the destruction of T4 lymphocytes, causing the body's immune system to collapse, making the body unable to defend against many diseases and resulting in death. Therefore, detection of HIV biomarkers or genes is very imp...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48G01N33/569G01N33/571G01N33/543C12Q1/6825
Inventor 朱烨温凯张翠玲杨兴东
Owner SHANDONG UNIV
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