An integrated automatic pretreatment device for high-throughput chromatography detection

Abstract

The invention discloses an integrated automatic pretreatment device for high-throughput chromatographic detection. Dairy pre-treatment efficiency, to achieve "one-stop" pre-treatment. The device includes an integrated centrifuge, a plunger pump, a three-way valve, and a multi-channel incubation-sampling device. The plunger pump provides power for the entire pre-treatment device, and realizes drawing of the target solution after treatment in the integrated centrifuge, filling of the incubation solution in the incubation-sampling device, and synchronous sampling after incubation; the three-way The valve provides a path for liquid delivery; the high-throughput chromatographic detection integrated automatic pretreatment device provided by the present invention can be used for processing dairy products, blood, tissues and other materials; it can be used for clinical diagnosis, inspection and quarantine, food safety testing, Sample pretreatment in many fields such as judicial identification and drug detection has broad market prospects.

Description

technical field [0001] The invention relates to a chemical reaction device, in particular to an integrated automatic pretreatment device for high-throughput chromatography detection, belonging to the technical field of biomedicine. Background technique [0002] Chromatography is the abbreviation of "chromatographic analysis". Taking the commonly used immunochromatography as an example, the principle is to fix the specific antibody to a specific zone of the nitrocellulose membrane, and then add the sample dropwise to the membrane. Under the action of the capillary, the sample moves forward along the membrane, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. At this time, if immunological nanoparticles such as colloidal gold or immunozyme are used for staining, or combined with marker molecules with Raman, chemiluminescence, upconversion luminescence, and time-resolved fluorescence ...

AI Technical Summary

Problems solved by technology

Taking the device for detecting chemical pollution indicators in dairy products (test strips, test cards) as an example, its disadvantages are: (1) Most of the detection indicators are directly measured on dairy products without pretreatment of milk samples. Simple and fast signal response, the biggest disadvantage is that the complex matrix environment of milk samples significantly restricts the detection sensitivity; (2) the inconsistency of the pre-processing methods of the detection indicators makes it difficult to achieve high-throughput determination of the dairy products to be tested

At present, the only high-throughput devices on the market are mostly high-throughput signal readers; (3) For the detection indicators that require pre-incubation, multi-channel simultaneous incubation and measurement before detection have not been realized, which significantly restricts the detection throughput.

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