Method for preparing pneumonia rat model and model evaluation method

Abstract

The invention discloses a method for preparing a pneumonia rat model and a model evaluation method, and belongs to the technical field of biology. The method for establishing the pneumonia rat model is non-invasive and safe to rats, does not damage other organs of rats in the whole process, and effectively avoids the death of the rats. Besides, the method has simple operation and high success rate. According to the pneumonia rat model established by the method, pseudomonas aeruginosa in bronchoalveolar lavage fluid is obviously increased, inflammatory factors IL-4 and TNF-alpha are obviously increased, the level of adhesion factor IFN-gamma is obviously reduced, and the number of leukocytes is obviously increased. Meanwhile, the lung index is also obviously increased, which indicates thatthe model is established well.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a pneumonia rat model and a model evaluation method. Background technique [0002] The prevention and treatment of pneumonia caused by drug-resistant bacteria is a difficult problem faced by the international medical community. In recent years, due to the frequent emergence of clinical multi-drug resistant strains and pan-drug resistant strains in my country, its prevention and control work is facing unprecedented challenges. The research and development of new effective prevention and control methods is urgent, especially the basic research based on the mechanism of drug resistance is imminent, and a stable and reliable model of pneumonia caused by drug-resistant bacteria is the first and necessary link. [0003] Due to considerations of cost and operability, rodents (mice, rats) are currently used as pneumonia model animals at home and abroad. Y...

AI Technical Summary

Problems solved by technology

However, there are still big problems in these methods, which are mainly reflected in the following aspects: 1. Although the method of incision of the trachea can guarantee the infection dose, as an invasive method, it is easy to cause damage to the animal organs during the operation and even lead to experimental failure. The animal died; 2. The operation is complicated, and each mouse needs to be operated on, so it is difficult to carry out experiments in large quantities; 3. The Pseudomonas aeruginosa strain used in the reported infection model is the standard strain PA01 from the United States, which cannot represent Epidemiological situation of popular PA strains in China

[0004] On the other hand, animal models need to be properly evaluated after the model is established, however, there is currently a lack of effective evaluation methods

Images