Ladybug attractant of aphid natural enemy
A natural enemy ladybug and attractant technology, applied in the direction of attracting pests, biocides, animal repellents, etc., can solve the problems of poor effect, instability, lack of applicability and specificity, etc., and achieve repeated effects Good sex, high stability, good lure effect
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Embodiment 1
[0027] Embodiment 1, aphid natural enemy ladybug attractant:
[0028] 0.01 mg of α-buchenene, 0.01 mg of α-humulene, 0.005 mg of E-β-farnesene, 0.07 mg of trans-2-hexenoic acid, 0.07 mg of benzaldehyde, 0.03 mg of isovaleraldehyde, and Dissolve 0.03 mg of alkenes, 0.12 mg of methyl salicylate, and 0.12 mg of eugenol in 20 μL of n-hexane, and mix well. The above solution was evenly added dropwise onto a quantitative filter paper sheet (5 cm in length and 1.5 cm in width) to obtain a compound loaded on a filter paper strip.
[0029] The behavioral response of ladybugs to this attractant was determined by wind tunnel experiments using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the...
Embodiment 2
[0048] Attractant 1: Mix 0.01mg 1-vinyl-1-methyl-2,4-di(prop-1-en-2-yl)cyclohexane, 0.005mg E-β-farnesene, 0.07mg trans- 2-hexenoic acid, 0.03 mg isovaleraldehyde, and 0.12 mg phenylacetaldehyde were dissolved in 20 μL of n-hexane solution, mixed evenly, and prepared as attractant 1, and the above solution was evenly added dropwise on a quantitative filter paper sheet (length 5 cm, width 1.5 cm).
[0049] Attractant 2: Dissolve 0.01mg α-humulene, 0.005mg E-β-farnesene, 0.07mg 2,5-hexanedione, 0.03mg dipentene, 0.12mg leaf alcohol in 20μL n-hexane solution, Mix evenly to prepare attractant 2. Take 20 μL of the above solution and drop evenly onto a quantitative filter paper sheet (length 5 cm, width 1.5 cm).
[0050] Attractant 3: Dissolve 0.01 mg α-buchenene, 0.005 mg E-β-farnesene, 0.07 mg benzaldehyde, 0.03 mg isovaleraldehyde, and 0.12 mg squalene in 20 μL n-hexane solution, mix well, and prepare To form attractant 3, take 20 μL of the above solution and evenly drop it ont...
Embodiment 3
[0059] Dissolve 0.01mg of α-buchenene, 0.005mg of E-β-farnesene, 0.07mg of benzaldehyde, 0.03mg of isovaleraldehyde, and 0.12mg of squalene in 20μL of n-hexane, mix well, take the above solution and add it dropwise onto a quantitative filter paper sheet (length 5cm, width 1.5cm).
[0060] The behavioral response of ladybugs to this attractant was determined using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the upper air outlet, and the wind speed in the wind tunnel is 0.3m / s. The whole bioassay process was carried out in a room at 28°C. Place the glass tube containing the ladybugs at the downwind end of the wind tunnel, and start timing for 30 minutes. Record the proportion of ...
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