Ladybug attractant of aphid natural enemy

A natural enemy ladybug and attractant technology, applied in the direction of attracting pests, biocides, animal repellents, etc., can solve the problems of poor effect, instability, lack of applicability and specificity, etc., and achieve repeated effects Good sex, high stability, good lure effect

Active Publication Date: 2019-06-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the invention patent "An Attractant for Seven-spot Ladybug" (CN201110180376.1), the raw materials of the attractant are composed of triple superphosphate, diammonium, and plant ash, which can attract adult seven-spot ladybugs, but the substances in this attractant have no The compound that specifically attracts ladybugs is a rough formula formed based on experience, which does not have applicability and specificity; patent CN201810000523.4 reports a red-ringed ladybug attractant for preventing and controlling paramecium, and the attractant is mainly paramecia The main volatile components produced by scales at different ages. This volatile is the kaimonin produced by pests. It can guide natural enemies to accurately locate pests at close range, but it involves long-distance luring. The effect of this formula is obviously poor. The number of ladybugs cannot be deployed in a large-scale overall area; CN201610297849.9 reports a kind of attractant for predatory natural enemy insects of aphids, which converts plant volatiles (mutual elements): α-pinene, eugenol, sophora The mixture of tree flower essential oil and chamomile essential oil has an attractive effect on ladybug heterochromia, ladybug six-spotted moon, hoverfly black belt, and lacewing; in CN201510579807.X, the pest-induced volatile matter (mutene): water Methyl sylate, 2-phenylethyl alcohol, phenylacetaldehyde, α-terpineol, etc. were mixed to prepare a natural enemy attractant for cotton aphids
Therefore, the effect of only using mutualene to lure natural enemies is unstable

Method used

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  • Ladybug attractant of aphid natural enemy
  • Ladybug attractant of aphid natural enemy
  • Ladybug attractant of aphid natural enemy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, aphid natural enemy ladybug attractant:

[0028] 0.01 mg of α-buchenene, 0.01 mg of α-humulene, 0.005 mg of E-β-farnesene, 0.07 mg of trans-2-hexenoic acid, 0.07 mg of benzaldehyde, 0.03 mg of isovaleraldehyde, and Dissolve 0.03 mg of alkenes, 0.12 mg of methyl salicylate, and 0.12 mg of eugenol in 20 μL of n-hexane, and mix well. The above solution was evenly added dropwise onto a quantitative filter paper sheet (5 cm in length and 1.5 cm in width) to obtain a compound loaded on a filter paper strip.

[0029] The behavioral response of ladybugs to this attractant was determined by wind tunnel experiments using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the...

Embodiment 2

[0048] Attractant 1: Mix 0.01mg 1-vinyl-1-methyl-2,4-di(prop-1-en-2-yl)cyclohexane, 0.005mg E-β-farnesene, 0.07mg trans- 2-hexenoic acid, 0.03 mg isovaleraldehyde, and 0.12 mg phenylacetaldehyde were dissolved in 20 μL of n-hexane solution, mixed evenly, and prepared as attractant 1, and the above solution was evenly added dropwise on a quantitative filter paper sheet (length 5 cm, width 1.5 cm).

[0049] Attractant 2: Dissolve 0.01mg α-humulene, 0.005mg E-β-farnesene, 0.07mg 2,5-hexanedione, 0.03mg dipentene, 0.12mg leaf alcohol in 20μL n-hexane solution, Mix evenly to prepare attractant 2. Take 20 μL of the above solution and drop evenly onto a quantitative filter paper sheet (length 5 cm, width 1.5 cm).

[0050] Attractant 3: Dissolve 0.01 mg α-buchenene, 0.005 mg E-β-farnesene, 0.07 mg benzaldehyde, 0.03 mg isovaleraldehyde, and 0.12 mg squalene in 20 μL n-hexane solution, mix well, and prepare To form attractant 3, take 20 μL of the above solution and evenly drop it ont...

Embodiment 3

[0059] Dissolve 0.01mg of α-buchenene, 0.005mg of E-β-farnesene, 0.07mg of benzaldehyde, 0.03mg of isovaleraldehyde, and 0.12mg of squalene in 20μL of n-hexane, mix well, take the above solution and add it dropwise onto a quantitative filter paper sheet (length 5cm, width 1.5cm).

[0060] The behavioral response of ladybugs to this attractant was determined using a wind tunnel setup. The wind tunnel device is 90cm long, 35cm wide and 35cm high. 30 minutes before the start of the test, the required ladybugs were placed in a glass tube, and the two ends of the glass tube were sealed with absorbent cotton to adapt to the environment in the wind tunnel. The compound loaded on the filter paper is placed at the upper air outlet, and the wind speed in the wind tunnel is 0.3m / s. The whole bioassay process was carried out in a room at 28°C. Place the glass tube containing the ladybugs at the downwind end of the wind tunnel, and start timing for 30 minutes. Record the proportion of ...

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PUM

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Abstract

The invention discloses a ladybug attractant of an aphid natural enemy. The attractant comprises ladybug sex pheromone, synomone and kairomone; the synomone is aphid-induced plant volatile matters; and the kairomone consists of aphid warning pheromone, aphid body surface extracts and aphid honey dew volatile matters. The ladybug attractant has good attraction effects, good effect repeatability andhigh stability in both laboratory and field experiments, and has broad spectrum for beneficial ladybugs such as coccinella septempunctata, harmonia axyridis, propylaea japonica and the like.

Description

technical field [0001] The invention relates to an attractant for pests, in particular to an attractant for ladybugs, a natural enemy of aphids. Background technique [0002] As one of the important means of comprehensive management of pests, biological control has the characteristics of high benefit, no pollution, safe for humans and animals, and conducive to environmental protection. With the development of modern agriculture, it has been widely valued. As a special resource, natural enemy insects It is also more and more accepted by people. Developed countries began to develop the natural enemy insect industry since the 1960s. At present, about 150 kinds of natural enemies are commercially produced and sold in the world, the main species are Trichogramma, grass ridge, predatory mite, small flower bug and ladybug, etc., and have achieved certain social and economic benefits. [0003] Ladybug is a general term for insects belonging to the family Coccinoptera. Ladybugs hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01N37/06A01N35/04A01N35/02A01N37/40A01N31/06A01N31/02A01N25/18A01P19/00
CPCA01N27/00A01N31/16A01N35/02A01N35/04A01N37/40A01N49/00A01N31/02A01N31/04A01N37/02
Inventor 赵金浩李中珊汪彪王忠燕程敬丽杜永均
Owner ZHEJIANG UNIV
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