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A kind of surface acoustic wave-based miniature bacterial lysis reactor and bacterial lysis method

A pyrolysis reactor and surface acoustic wave technology, applied in specific-purpose bioreactors/fermenters, bioreactor/fermenter combinations, methods of stress-stimulating microbial growth, etc. Large, long processing time and other problems, to achieve the effect of low attenuation, small sample volume, short rupture time

Active Publication Date: 2020-11-10
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the methods used for bacterial lysis mainly include lysozyme lysis, mechanical lysis, ultrasonic lysis, centrifugation, etc., but these methods have large sample volume and long processing time, and are not suitable for rapid on-site analysis.

Method used

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  • A kind of surface acoustic wave-based miniature bacterial lysis reactor and bacterial lysis method
  • A kind of surface acoustic wave-based miniature bacterial lysis reactor and bacterial lysis method
  • A kind of surface acoustic wave-based miniature bacterial lysis reactor and bacterial lysis method

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Effect test

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[0037]Connect the injection hole 7 and the product outflow hole 9 at the upper cover plate 2 of the miniature bacterial lysis reactor based on the surface acoustic wave to the syringe with the bacterial suspension to be cracked on the syringe pump and the catheter for the bacterial lysate collection tube respectively. connected. Set the flow rate of the syringe pump to 1 mL / h. Input a pulse signal with a duty cycle of 33% and a frequency of 333Hz into the pulse signal generator. Connect the positive and negative poles of the output signal of the power amplifier to the interdigitated electrodes on the surface acoustic wave generating structure 3 respectively, adjust the output signal frequency of the pulse signal generator to be 13MHz, and the power input to the surface acoustic wave generating structure 3 is 14W; Turn on the signal control system and the syringe pump to lyse the bacteria. Escherichia coli, a commonly used experimental bacterium, was selected. Driven by the ...

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Abstract

The invention discloses a micro bacteria cracking reactor based on surface acoustic waves and a bacteria cracking method. The reactor comprises a lower bottom plate and an upper cover plate which arealigned and bonded together; a group of surface acoustic wave generation structures are arranged on the lower bottom plate; a groove is formed in one side of the upper cover plate, and a micro-channelstructure allowing liquid to flow through is arranged in the middle of the upper cover plate; a signal control system is used for controlling the surface acoustic wave generation structures; the micro-channel structure comprises a sample introduction hole, a reaction area and a product outflow hole. The method is suitable for cracking different types of bacteria, does not need any chemical reagent, and avoids the pollution of the chemical reagent to a cracked product.

Description

technical field [0001] The invention belongs to the technical field of micro-nano manufacturing application, relates to a detection device of a biochemical sample, in particular to a surface acoustic wave-based miniature bacterial lysis reactor and a bacterial lysis method. Background technique [0002] The study of bacteria at the molecular level is of great significance in disease pathology and drug screening. When analyzing bacteria, the bottleneck problem is how to lyse bacteria quickly and efficiently. The current methods for bacterial lysis mainly include lysozyme lysis, mechanical lysis, ultrasonic lysis, centrifugation, etc., but these methods have large sample volume and long processing time, and are not suitable for rapid on-site analysis. Contents of the invention [0003] The purpose of the present invention is to provide a micro-bacteria cracking reactor based on surface acoustic waves and a bacterial cracking method, which utilizes the acoustic radiation for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12M1/36C12N1/06
Inventor 郑腾飞芦海伟胡家栋周文陈家欢王朝晖
Owner XI AN JIAOTONG UNIV
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