Acetylcholine esterase inhibitor prepared by using cyrtomium rhizome, preparation method and application

A technology of acetylcholinease and inhibitor, applied in the field of acetylcholinease inhibitor, can solve problems such as unseen application

Active Publication Date: 2019-06-28
JINGGANGSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Weinan Materia Medica" records that the main application value of Miyu Guanzhong is detoxification, hemostasis, and detoxification, and "Shaanxi Chinese Herbal Medicine" records that the main application value of Miyu Guanzhong is heat-clearing and detoxifying, hemostasis and killing insects, but it is not See its application in the preparation of acetylcholinesterase inhibitors

Method used

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  • Acetylcholine esterase inhibitor prepared by using cyrtomium rhizome, preparation method and application
  • Acetylcholine esterase inhibitor prepared by using cyrtomium rhizome, preparation method and application
  • Acetylcholine esterase inhibitor prepared by using cyrtomium rhizome, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing acetylcholinease inhibitors by utilizing Rhizoma Rhizoma Rhizome, comprising the following steps:

[0028] (1) Wash the whole plant of Rhizoma Rhizoma Rhizome and dry it at 55°C until the moisture content is ≤10%. Take 2kg of dried Rhizoma Rhizoma Rhizoma Rhizome, crush it, and use ethanol equivalent to 6 times the mass of the whole plant of Rhizoma Rhizoma Rhizome- Extract 3 times with methanol mixed solution at room temperature, 3 hours each time, filter, collect all the extracts, and concentrate the extracts under reduced pressure to obtain 182g of the extract of A. After the extract is dried, the crude extract of Rhododendron japonicus is obtained; the volume ratio of ethanol and methanol in the ethanol-methanol mixed solution is 95:5;

[0029] (2) Add water to dissolve the herb extract of Miyuguanzhong to obtain 2L of suspension, extract with petroleum ether and ethyl acetate in sequence, and collect respectively to obtain petroleum ether ext...

Embodiment 2

[0033] A method for preparing acetylcholinease inhibitors by utilizing Rhizoma Rhizoma Rhizome, comprising the following steps:

[0034] Wash and dry the whole plant of Rhizoma miltiorrhiza, and dry it at 60°C until the water content is ≤15%. Take 2kg of the dried whole plant of Rhizoma miltiorrhiza, grind it and mix it with ethanol-methanol equivalent to 8 times the mass of the whole plant of Rhizoma mercifolia The solution was extracted at room temperature for 3 times, each time for 2 hours, filtered, the extract was collected, and the extract was concentrated under reduced pressure to obtain 175g of the whole plant extract of A. Rhizome crude extract; the volume ratio of ethanol and methanol in the ethanol-methanol mixed solution is 95:5; the crude extract of Rhizoma radix contains compounds of formula 1 to formula 6.

Embodiment 3

[0036] A method for preparing acetylcholinease inhibitors by utilizing Rhizoma Rhizoma Rhizome, comprising the following steps:

[0037] Wash the whole plant of Rhizoma Rhizome and dry it at 45°C until the water content is ≤10%. Take 2kg of dried Rhizoma Rhizoma Rhizome and mix it with ethanol-methanol equivalent to 7 times the mass of the whole plant of Rhizoma Rhizome. The solution was leached twice at room temperature, each time for 2 hours, filtered, the extract was collected, and the extract was concentrated under reduced pressure to obtain 175g of the whole herb extract of A. Rhizome crude extract; the volume ratio of ethanol and methanol in the ethanol-methanol mixed solution is 95:5; the crude extract of Rhizoma radix contains compounds of formula 1 to formula 6.

[0038] In order to verify the effect of the present invention, we have detected the inhibitory effect of each compound on the activity of acetylcholinesterase (Ellman colorimetric method). The result is as ...

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Abstract

The invention belongs to the technical field of acetylcholine esterase inhibitors, and particularly relates to an extraction method and application of an acetylcholine esterase inhibitor prepared by using cyrtomium rhizome. The acetylcholine esterase inhibitor prepared by using the cyrtomium rhizome contains the following six compounds of kaempferol, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-D-rutinoside, frangula emodin, daucosterol and beta-sitosterol. The cyrtomium rhizome can be used for developing a novel acetylcholine esterase inhibitor, provides a drug candidate for the prevention and treatment of Alzheimer's disease (AD), and has important economic value and social significance for the development and utilization of medicinal plants.

Description

technical field [0001] The invention belongs to the technical field of acetylcholinesterase inhibitors, and in particular relates to an acetylcholinesterase inhibitor prepared by using Rhododendron japonicus, a preparation method and an application. Background technique [0002] Alzheimer's disease (also known as Alzheimer's, Alzheimer's Disease, AD) is one of the important diseases that seriously threaten human life and health after cardiovascular diseases and tumors. Blockage of the cholinergic system can cause decline in memory and learning , similar to amnesia in normal old age. If the central cholinergic activity is strengthened, the learning and memory ability of the elderly can be improved. Therefore, the development of drugs that can improve the cholinergic system will greatly improve dementia. At present, the four commonly used acetylcholinesterase inhibitors include tacrine and exon. Tacrine can delay the course of the disease for 6 to 12 months, and the common a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/7048A61K31/122A61K31/704A61K31/575A61P25/28
Inventor 殷帅文朱立成
Owner JINGGANGSHAN UNIVERSITY
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