Dendrobium candidum tissue culture rapid propagation method

Abstract

The invention discloses a dendrobium candidum tissue culture and rapid propagation method. The method comprises the following steps of preparation of capsule seeds, inducting of germination and differentiation of the seeds, proliferation of seedlings, rooting of strong seedlings and transplanting. As the method mainly adopts the three-step seedling promoting culture method of seed induction culture, seedling proliferation culture and strong seedling rooting culture to replace the traditional four-step proliferation method of dendrobium candidum tissue culture, the production cycle of seedlingscan be effectively shortened by two to three months, and thus the time, labor and consumption cost in all links of production are reduced. In the method, a pure dark culture method is used for culturing in about the first two weeks of each expanding propagation step to effectively increase the growth rate, and a three-generation proliferation method is adopted so that the genes in mother strainsare better duplicated to ensure the purity of the seedling species. In the method, the formula of a culture medium is improved, and by increasing a potassium fertilizer and a silicon fertilizer, particularly at the strong seedling stage, the stoutness and uniformity of the bottled seedlings are improved and the survival rate of the seedlings is increased, and thus the market competitiveness of theseedlings is enhanced.

Description

technical field [0001] The invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to a rapid propagation method of dendrobium officinale through tissue culture. Background technique [0002] Dendrobium officinale is a traditional rare and precious Chinese medicinal material. It is known as the "life-saving celestial grass" because of its anti-tumor, anti-aging, anti-vocal cord fatigue, dilating blood vessels, and enhancing human immunity. "The first. With the continuous development of the health value of Dendrobium candidum, the demand for Dendrobium candidum in the medicinal material market is gradually increasing. However, due to long-term over-harvesting, China's wild resources have been exhausted, and the traditional asexual reproduction method has a low reproduction coefficient and cannot satisfy large-scale production. Cultivation of seedlings is required. At present, tissue culture seedlings have become the m...

AI Technical Summary

Problems solved by technology

With the continuous development of the health value of Dendrobium candidum, the demand for Dendrobium candidum in the medicinal material market is gradually increasing. However, due to long-term over-harvesting, China's wild resources have been exhausted, and the traditional asexual reproduction method has a low reproduction coefficient and cannot satisfy large-scale production. The cultivation of seedlings is needed, and tissue cultured seedlings have become the main source of seedlings for Dendrobium candidum cultivation

At present, there are many studies on tissue culture and rapid propagation of Dendrobium candidum, but most of them have problems such as complex medium components, long seedling cultivation cycle, labor-intensive, high cost of tissue culture seedlings, etc., which seriously restrict the industrialization of Dendrobium candidum.