Application of pyrazine in attracting citrus psylla and citrus psylla attractant
A citrus psyllid and attractant technology, which is applied in the directions of attracting pests, applications, biocides, etc., can solve the problems of less compounds of citrus psyllids and the like
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Embodiment 1
[0036] (1) Use rubber tubes with a length of 30cm to connect the taste source bottles with different concentrations of pyrazine solutions at the different mouths of the four-necked flasks; the pyrazine solutions are prepared with ultrapure water, and the volume concentrations are 0% and 0.1% respectively , 1% and 10%, of which 0% is a blank control;
[0037] (2) Shade the four-necked flask containing the psyllid, put about 40 adults at the bottom of the flask at a time, and use the single-necked bottleneck of the four-necked flask as the boundary. The number of citrus psyllids at each bottle mouth and the total amount of four bottle mouth citrus psyllids were recorded every 10 minutes, and the induction efficiency was calculated. Each experiment was replaced with unused psyllids and repeated three times.
[0038] The statistics of the results are shown in Table 1.
[0039] Table 1 The induction efficiency (%) of different concentrations of pyrazine to citrus psyllid
[0040...
Embodiment 2
[0043] (1) Connect zone 1 and zone 2 of the six-arm olfactory instrument to the flavor source bottle, leave the remaining four arms empty and close the arm mouth, and zone 2 is set as the control zone;
[0044] (2) Turn on the vacuum pump, adjust the air flow rate of each arm of the six-arm olfactometer to 80mL / min, and wait until the air flow is stable;
[0045] (3) Put 1% pyrazine solution (solvent is ultrapure water) in the odor source bottle corresponding to area 1, and put an equal amount of ultrapure water in the odor source bottle corresponding to area 2 as a control.
[0046] Each experimental test was carried out head by head, and the number of times the psyllid entered different areas and the number of times it was in the area when it stayed motionless for 3 minutes was recorded as the test result of the citrus psyllid's chemotactic response to different volatile compounds. The test results are shown in Table 2 Show.
[0047] Table 2 Comparison of the number of time...
Embodiment 3
[0051] In insect cages with a size of 75*75*90 (cm), combined with yellow boards (size of 15*20 cm) to conduct insect trap experiments, the amount of drug coating on each yellow board was about 1mL. Pyrazine with a volume concentration of 0.1% (solvent is ultrapure water) was coated on the yellow board, and hung symmetrically with the yellow board without medicine in the insect cage. In the middle of the insect cage, put a plant of Murata japonica, and put about 20 psyllids in the cage, and add about 1mL of medicine on the yellow board the next day. Count the number of citrus psyllid adults on the yellow board every day, and the experimental results are shown in Table 3:
[0052] Table 3 0.1% pyrazine trap results
[0053] project first day the next day third day control 3 3 3 pyrazine 8 13 16
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