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Application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry

A technology of matrix-assisted laser and time-of-flight mass spectrometry, which is applied in the field of mass spectrometry detection, can solve problems such as the complexity of the synthesis process, and achieve the effect of broadening the scope of use

Inactive Publication Date: 2019-08-27
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These inorganic nanomaterials are used as the matrix of MALDI, so that MALDI can be successfully used in the determination of the molecular weight of small molecules, which broadens the application range of MALDI. However, some of these nanomaterials need to prepare composite materials, and the synthesis process is complicated.

Method used

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  • Application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • Application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • Application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Gold clusters as substrates for the detection of amino acids

[0044] Using amino acids as small molecules, the ability of gold clusters as substrates to analyze the molecular weight of small molecules by MALDI-TOF-MS was evaluated. Figure 5 It is the mass spectrum of four mixed amino acids (threonine, histidine, alanine, phenylalanine). from Figure 5 It can be found that the matrix interference effect is suppressed, the fragmentation peak hardly exists, and shows a higher signal ratio (S / N), resolution (Resolution), and signal intensity (Intensity), as shown in Table 1. The results show that gold clusters can be used as the matrix of MALDI for the determination of the molecular weight of amino acid small molecules.

[0045] Table 1 Four kinds of amino acid detection results

[0046]

[0047]

Embodiment 2

[0049] Gold clusters as substrates for the detection of cyclodextrins

[0050] In order to better verify whether gold clusters have a wide range of application capabilities as a matrix, we continued to investigate gold clusters as a matrix for testing the molecular weight of two cyclodextrins (β and γ-CD). The results are as follows Image 6 shown. Gold clusters can be used as the matrix of MALDI to test the molecular weight of cyclodextrin and the analysis results are shown in the table. It can be seen from Table 2 that the S / N of the two cyclodextrins are 11 and 9, respectively, both greater than 3, indicating that gold clusters can be used as a MALDI matrix to test the molecular weight of cyclodextrins.

[0051] Table 2 Two kinds of cyclodextrin detection results

[0052] m / z S / N Resolution Intensity β-CD 1158.477[M+H] +

Embodiment 3

[0054] Gold clusters as substrates for the detection of polyethylene glycol

[0055] MALDI-TOF-MS is often used to analyze the molecular weight of some polymers. In order to examine the application range of gold clusters as a matrix, this example uses polyethylene glycol (PEG) (HO-(CH2CH2O)n-H ) as the target analyte, using gold clusters as the matrix, mass spectrometry test, the results are as follows Figure 7 -As shown in A, B, C, D. Among them, the molecular weight distribution ranges of PEG 2000, 4000, 6000 and 8000 are 1800-2200Da, 3600-4400Da, 5500-7500, 7500-8500 respectively. It can be seen from the figure that except for PEG8000, the molecular weight distributions of other test results are, PEG 2000: 1400-2300; PEG4000: 3000-5000; PEG 6000: 5000-7000. Although the signal resolution of PEG 8000 is low, the rest of the signals are strong and have good resolution. The distribution of the tested PEG signal is better than that of DHB (a matrix commonly used in polymer m...

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Abstract

The present invention discloses an application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The application utilizes the gold clusters as a matrix,and uses the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze small amino acid molecules, cyclodextrin, polyethylene glycol and the like. Resultsindicates that the gold clusters with bovine serum albumin (BSA) as templates are successfully synthesized, and the gold clusters are used as the matrix, molecular weight of the small molecule molecules, the cyclodextrin, the polyethylene glycol and the like are respectively determined by using the MALDI-TOF-MS, and the results broadens scope of use of the gold clusters.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and in particular relates to the application of gold clusters in matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection. Background technique [0002] Matrix-assisted laser desorption mass spectrometry was proposed by Karas and Hillenkamp. Because of its high ionization efficiency, high sensitivity, rapidity, high throughput and easy automation, it has become a powerful tool for the analysis of polymers, biomolecules. The basic working principle of mass spectrometry (MALDI) is: first mix the sample with the matrix, point it on the special sample target, after drying and crystallization, use pulsed laser to irradiate the eutectic formed by the sample and the corresponding matrix, and the matrix can effectively absorb The energy of the laser is delivered to the biomolecules, during which the protons are transferred to or from the biomolecules, th...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N33/68
CPCG01N27/64G01N33/6851
Inventor 秦小焕胡坤何允凤
Owner GUANGXI NORMAL UNIV
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