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Method for establishing hplc fingerprints of fine-stemmed herbs and their fingerprints

A fingerprint and thin-stem vanilla technology, which is applied in material separation, analysis materials, instruments, etc., can solve the problems of thin-stem vanilla lack and weakness, and achieve the effect of ensuring products, ensuring relative stability, and improving the quality evaluation system

Active Publication Date: 2021-09-21
海南曼华科贸有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches on the quality control of herb and mainly focus on the content determination of a single index component, which is very weak for the quality control of herb.

Method used

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  • Method for establishing hplc fingerprints of fine-stemmed herbs and their fingerprints
  • Method for establishing hplc fingerprints of fine-stemmed herbs and their fingerprints
  • Method for establishing hplc fingerprints of fine-stemmed herbs and their fingerprints

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Chromatographic conditions and system suitability test using octadecylsilane bonded silica gel as filler (column length 25cm, inner diameter 4.6 mm, particle size 5μm); acetonitrile as mobile phase A, 0.3% phosphoric acid solution as mobile phase B. Carry out gradient elution as specified in the table below; the detection wavelength is 254nm; the column temperature is 30°C; the flow rate is 1.0ml per minute.

[0106]

[0107] Preparation of reference solution

[0108] Precise measurement of fine-stemmed vanilla reference substances quercetin-3-O-(2”, 6”-di-O-α-rhamnopyranosyl)-β-galactoside, kaempferol-3-O- [2-Glucopyranosyl (1→3)-rhamnopyranose-6-rhamnopyranose]-β-D-galactopyranoside, kaempferol-3-O-{[β-D -Glucopyranosyl(1→3)]-4-O-(E-p-coumarin)}-α-L-rhamnopyranose-(1→6)-β-D-glucopyranoside-7 -O-(4-O-acetyl)-α-L-rhamnopyranoside, kaempferol-3-O-{[β-D-xylopyranosyl (1→3)-α-L- Rhamnopyranose (1→6)][α-L-rhamnopyranose-(1→2)]}-β-D-3-trans-p-coumaroyl glucopyranoside ...

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Abstract

The invention discloses a method for establishing a fingerprint of herbal medicinal material with thin stems and its fingerprint. The method comprises the following steps: (1) preparation of a reference substance solution; (2) preparation of a test solution: (3) fingerprint : (4) similarity evaluation: the inventive method is simple, quick, accurate, good in reproducibility; It provides powerful guarantee for the comprehensive and effective control of the fine-stemmed herb quality.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing fingerprints of fine-stemmed herbs and fingerprints thereof. Background technique [0002] Traditional Chinese medicine fingerprint refers to the spectrum that can mark the chemical characteristics of certain Chinese medicinal materials or traditional Chinese medicine preparations after proper processing and certain analysis methods. It can more comprehensively reflect the types and quantities of chemical components contained in medicinal materials, and effectively reflect the integrity and comprehensive effects of traditional Chinese medicine ingredients. It has been widely used in the analysis, identification and quality control of traditional Chinese medicine due to its fast and accurate characteristics. One of the most effective technical means at this stage has also been recognized by the international...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 杨毅生洪挺钟瑞建章光文王栋钱媛张昆艳
Owner 海南曼华科贸有限责任公司
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